The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney
Introduction: The diameter and area of the proximal convoluted tubule (PCT) and the distal convoluted tubule (DCT) are of the main parameters analyzed in stereological studies of the kidney. However, there is no consensus about if the PCT and DCT should be considered circular or elliptical in shape....
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Karger Publishers
2021-03-01
|
| Series: | Kidney & Blood Pressure Research |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/515051 |
| _version_ | 1818412088490459136 |
|---|---|
| author | Marta Ortega-Martinez Vanessa Gutierrez-Davila Esthefania Gutierrez-Arenas Alberto Niderhauser-Garcia Ricardo M. Cerda-Flores Gilberto Jaramillo-Rangel |
| author_facet | Marta Ortega-Martinez Vanessa Gutierrez-Davila Esthefania Gutierrez-Arenas Alberto Niderhauser-Garcia Ricardo M. Cerda-Flores Gilberto Jaramillo-Rangel |
| author_sort | Marta Ortega-Martinez |
| collection | DOAJ |
| description | Introduction: The diameter and area of the proximal convoluted tubule (PCT) and the distal convoluted tubule (DCT) are of the main parameters analyzed in stereological studies of the kidney. However, there is no consensus about if the PCT and DCT should be considered circular or elliptical in shape. Objective: To analyze if there are significant differences in the diameter and area of the PCT and DCT, depending on whether they are considered circular or elliptical. Methods: Paraffin-embedded sections of kidneys from CD1 mice were stained with hematoxylin and eosin and examined using a light microscope. Images were captured using a camera linked to image analysis software. A short diameter (d) and a long diameter (D) were measured in both PCT and DCT. A small circular area (SCA), a large circular area (LCA), and an elliptical area (EA) were calculated with mathematical formulas that incorporate d and D values, while a program area (PA) was provided by the software. Results: There was a significant difference between d and D in both PCT (F = 1.354, Sig = 0.000) and DCT (F = 4.989, Sig = 0.000). Also, there were significant differences in the tubular areas in both PCT (F = 34.843, Sig = 0.000) and DCT (F = 22.390, Sig = 0.000); circular areas were different from elliptical areas (SCA and LCA vs. EA and PA). Conclusion: The convoluted tubules of the nephron must not be considered circular, but rather elliptical; care should be taken every time the tubules are analyzed in stereological studies of the kidney, especially when evaluating their diameters and areas. |
| first_indexed | 2024-12-14T10:41:45Z |
| format | Article |
| id | doaj.art-6222b9ce0e054a158bab441dc0fb65f1 |
| institution | Directory Open Access Journal |
| issn | 1420-4096 1423-0143 |
| language | English |
| last_indexed | 2024-12-14T10:41:45Z |
| publishDate | 2021-03-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Kidney & Blood Pressure Research |
| spelling | doaj.art-6222b9ce0e054a158bab441dc0fb65f12022-12-21T23:05:40ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432021-03-0146222923510.1159/000515051515051The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the KidneyMarta Ortega-Martinez0Vanessa Gutierrez-Davila1Esthefania Gutierrez-Arenas2Alberto Niderhauser-Garcia3Ricardo M. Cerda-Flores4Gilberto Jaramillo-Rangel5Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, MexicoDepartment of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, MexicoSchool of Biology, Autonomous University of Sinaloa, Culiacan, MexicoDepartment of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, MexicoSchool of Nursing, Autonomous University of Nuevo Leon, Monterrey, MexicoDepartment of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, MexicoIntroduction: The diameter and area of the proximal convoluted tubule (PCT) and the distal convoluted tubule (DCT) are of the main parameters analyzed in stereological studies of the kidney. However, there is no consensus about if the PCT and DCT should be considered circular or elliptical in shape. Objective: To analyze if there are significant differences in the diameter and area of the PCT and DCT, depending on whether they are considered circular or elliptical. Methods: Paraffin-embedded sections of kidneys from CD1 mice were stained with hematoxylin and eosin and examined using a light microscope. Images were captured using a camera linked to image analysis software. A short diameter (d) and a long diameter (D) were measured in both PCT and DCT. A small circular area (SCA), a large circular area (LCA), and an elliptical area (EA) were calculated with mathematical formulas that incorporate d and D values, while a program area (PA) was provided by the software. Results: There was a significant difference between d and D in both PCT (F = 1.354, Sig = 0.000) and DCT (F = 4.989, Sig = 0.000). Also, there were significant differences in the tubular areas in both PCT (F = 34.843, Sig = 0.000) and DCT (F = 22.390, Sig = 0.000); circular areas were different from elliptical areas (SCA and LCA vs. EA and PA). Conclusion: The convoluted tubules of the nephron must not be considered circular, but rather elliptical; care should be taken every time the tubules are analyzed in stereological studies of the kidney, especially when evaluating their diameters and areas.https://www.karger.com/Article/FullText/515051distal convoluted tubulekidneymouseproximal convoluted tubule |
| spellingShingle | Marta Ortega-Martinez Vanessa Gutierrez-Davila Esthefania Gutierrez-Arenas Alberto Niderhauser-Garcia Ricardo M. Cerda-Flores Gilberto Jaramillo-Rangel The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney Kidney & Blood Pressure Research distal convoluted tubule kidney mouse proximal convoluted tubule |
| title | The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney |
| title_full | The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney |
| title_fullStr | The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney |
| title_full_unstemmed | The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney |
| title_short | The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney |
| title_sort | convoluted tubules of the nephron must be considered elliptical and not circular in stereological studies of the kidney |
| topic | distal convoluted tubule kidney mouse proximal convoluted tubule |
| url | https://www.karger.com/Article/FullText/515051 |
| work_keys_str_mv | AT martaortegamartinez theconvolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT vanessagutierrezdavila theconvolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT esthefaniagutierrezarenas theconvolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT albertoniderhausergarcia theconvolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT ricardomcerdaflores theconvolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT gilbertojaramillorangel theconvolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT martaortegamartinez convolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT vanessagutierrezdavila convolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT esthefaniagutierrezarenas convolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT albertoniderhausergarcia convolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT ricardomcerdaflores convolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney AT gilbertojaramillorangel convolutedtubulesofthenephronmustbeconsideredellipticalandnotcircularinstereologicalstudiesofthekidney |