Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus
Diabetic peristalsis disorders are common complications in diabetes mellitus type 2. Disturbance of interstitial cells of Cajal (ICC) caused by metabolic changes in diabetes could explain the symptoms of diabetic gastroenteropathy. Although heterogenous interstitial cell types represent only 5% of t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2023-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300012V.pdf |
| _version_ | 1797393732674256896 |
|---|---|
| author | Veličkov Aleksandra I. Petrović Vladimir Đorđević Branka Veličkov Asen V. Petrović Aleksandar Lazarević Milica Cvetković Julija |
| author_facet | Veličkov Aleksandra I. Petrović Vladimir Đorđević Branka Veličkov Asen V. Petrović Aleksandar Lazarević Milica Cvetković Julija |
| author_sort | Veličkov Aleksandra I. |
| collection | DOAJ |
| description | Diabetic peristalsis disorders are common complications in diabetes mellitus type 2. Disturbance of interstitial cells of Cajal (ICC) caused by metabolic changes in diabetes could explain the symptoms of diabetic gastroenteropathy. Although heterogenous interstitial cell types represent only 5% of the cell population of the muscle layer in the gastrointestinal tract (GIT), they are important for conducting electrical signals and regulating muscle excitability. The aim of this study was to investigate the alterations of the myenteric and intramuscular ICCs in the gaster of rats with diabetes mellitus type 2 (DMT2), as well as determine their distribution in relation to smooth muscle cells and enteric nerve structures. Male Wistar rats were used and DT2 was induced by streptozotocin-nicotinamide (STZ-NA) application. The stomach specimens were exposed to type III transmembrane tyrosine kinase (c-KIT), neurofilament (NF-M) protein and desmin antibodies to investigate the ICC, enteric neurons and smooth muscle cells. Morphological changes of the cells were quantified by the numerical areal density of intramuscular ICC, the ICC score of myenteric ICC and the volume density of nerve fibers. In conclusion, a statistically significant decrease in the number of intramuscular ICC and myenteric ICC without nerve fiber loss were observed in all stomach regions in rats with STZ-NA-induced DMT2. |
| first_indexed | 2024-03-09T00:07:10Z |
| format | Article |
| id | doaj.art-622c3f4309804ae5953871e40db4d692 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-09T00:07:10Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-622c3f4309804ae5953871e40db4d6922023-12-12T13:06:41ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175214115410.2298/ABS230220012V0354-46642300012VDistribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitusVeličkov Aleksandra I.0https://orcid.org/0000-0001-9736-860XPetrović Vladimir1https://orcid.org/0000-0001-8618-694XĐorđević Branka2Veličkov Asen V.3https://orcid.org/0000-0003-1400-5841Petrović Aleksandar4Lazarević Milica5https://orcid.org/0000-0002-0031-6211Cvetković Julija6University of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Biochemistry, Niš, SerbiaUniversity Clinical Center Niš, Clinic for Orthopedic Surgery and Traumatology, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity Clinical Center Niš, Center for Pathology, Niš, SerbiaDiabetic peristalsis disorders are common complications in diabetes mellitus type 2. Disturbance of interstitial cells of Cajal (ICC) caused by metabolic changes in diabetes could explain the symptoms of diabetic gastroenteropathy. Although heterogenous interstitial cell types represent only 5% of the cell population of the muscle layer in the gastrointestinal tract (GIT), they are important for conducting electrical signals and regulating muscle excitability. The aim of this study was to investigate the alterations of the myenteric and intramuscular ICCs in the gaster of rats with diabetes mellitus type 2 (DMT2), as well as determine their distribution in relation to smooth muscle cells and enteric nerve structures. Male Wistar rats were used and DT2 was induced by streptozotocin-nicotinamide (STZ-NA) application. The stomach specimens were exposed to type III transmembrane tyrosine kinase (c-KIT), neurofilament (NF-M) protein and desmin antibodies to investigate the ICC, enteric neurons and smooth muscle cells. Morphological changes of the cells were quantified by the numerical areal density of intramuscular ICC, the ICC score of myenteric ICC and the volume density of nerve fibers. In conclusion, a statistically significant decrease in the number of intramuscular ICC and myenteric ICC without nerve fiber loss were observed in all stomach regions in rats with STZ-NA-induced DMT2.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300012V.pdfdiabetes mellitus type 2interstitial cells of cajalstomach (gaster)streptozotocinnicotinamide |
| spellingShingle | Veličkov Aleksandra I. Petrović Vladimir Đorđević Branka Veličkov Asen V. Petrović Aleksandar Lazarević Milica Cvetković Julija Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus Archives of Biological Sciences diabetes mellitus type 2 interstitial cells of cajal stomach (gaster) streptozotocin nicotinamide |
| title | Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus |
| title_full | Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus |
| title_fullStr | Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus |
| title_full_unstemmed | Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus |
| title_short | Distribution of interstitial cells of Cajal and nerve fibers in rat stomach in streptozotocin-nicotinamide-induced diabetes mellitus |
| title_sort | distribution of interstitial cells of cajal and nerve fibers in rat stomach in streptozotocin nicotinamide induced diabetes mellitus |
| topic | diabetes mellitus type 2 interstitial cells of cajal stomach (gaster) streptozotocin nicotinamide |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300012V.pdf |
| work_keys_str_mv | AT velickovaleksandrai distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus AT petrovicvladimir distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus AT đorđevicbranka distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus AT velickovasenv distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus AT petrovicaleksandar distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus AT lazarevicmilica distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus AT cvetkovicjulija distributionofinterstitialcellsofcajalandnervefibersinratstomachinstreptozotocinnicotinamideinduceddiabetesmellitus |