Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass
Abstract Background Hydrogen is a promising source of alternative energy. Fermentative production is more feasible because of its high hydrogen generation rate, simple operating conditions, and utilization of various organic wastes as substrates. The most significant constraint for biohydrogen produ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02193-0 |
| _version_ | 1797450977590116352 |
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| author | Eman S. E. Aldaby Aya H. A. Mahmoud Haitham M. El-Bery Maysa M. Ali Ahmed A. Shoreit Asmaa M. M. Mawad |
| author_facet | Eman S. E. Aldaby Aya H. A. Mahmoud Haitham M. El-Bery Maysa M. Ali Ahmed A. Shoreit Asmaa M. M. Mawad |
| author_sort | Eman S. E. Aldaby |
| collection | DOAJ |
| description | Abstract Background Hydrogen is a promising source of alternative energy. Fermentative production is more feasible because of its high hydrogen generation rate, simple operating conditions, and utilization of various organic wastes as substrates. The most significant constraint for biohydrogen production is supplying it at a low cost with fewer impurities. Results Leaf biomass of Calotropis procera was used as a feedstock for a dark fermentative production of hydrogen by Bacillus coagulans AH1 (MN923076). The optimum operation conditions for biohydrogen production were 5.0% substrate concentrationand pH 9.0, at 35 °C. In which the biohydrogen yield was 3.231 mmol H2/g dry biomass without any pretreatments of the biomass. A freshwater microalga Oscillatroia sp was used for upgrading of the produced biohydrogen. It sequestrated 97 and 99% % of CO2 from the gas mixture when it was cultivated in BG11 and BG11-N media, respectively After upgrading process, the residual microalgal cells exhibited 0.21mg/mL of biomass yield,high content of chlorophyll-a (4.8 µg/mL) and carotenoid (11.1 µg/mL). In addition to Oscillatroia sp residual biomass showed a lipid yield (7.5–8.7%) on the tested media. Conclusion Bacillus coagulans AH1 is a promising tool for biohydrogen production avoiding the drawbacks of biomass pretreatment. Oscillatroia sp is encouraged as a potent tool for upgrading and purification of biohydrogen. These findings led to the development of a multiproduct biorefinery with zero waste that is more economically sustainable. Graphical Abstract |
| first_indexed | 2024-03-09T14:48:18Z |
| format | Article |
| id | doaj.art-62311a78992742a8892b551daf2e6a8b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-09T14:48:18Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-62311a78992742a8892b551daf2e6a8b2023-11-26T14:38:01ZengBMCMicrobial Cell Factories1475-28592023-09-0122111410.1186/s12934-023-02193-0Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomassEman S. E. Aldaby0Aya H. A. Mahmoud1Haitham M. El-Bery2Maysa M. Ali3Ahmed A. Shoreit4Asmaa M. M. Mawad5Botany and Microbiology Department, Faculty of Science, Assiut UniversityBotany and Microbiology Department, Faculty of Science, Assiut UniversityGreen Hydrogen Production Laboratory, Chemistry Department, Faculty of Science, Assiut UniversityBotany and Microbiology Department, Faculty of Science, Assiut UniversityBotany and Microbiology Department, Faculty of Science, Assiut UniversityBotany and Microbiology Department, Faculty of Science, Assiut UniversityAbstract Background Hydrogen is a promising source of alternative energy. Fermentative production is more feasible because of its high hydrogen generation rate, simple operating conditions, and utilization of various organic wastes as substrates. The most significant constraint for biohydrogen production is supplying it at a low cost with fewer impurities. Results Leaf biomass of Calotropis procera was used as a feedstock for a dark fermentative production of hydrogen by Bacillus coagulans AH1 (MN923076). The optimum operation conditions for biohydrogen production were 5.0% substrate concentrationand pH 9.0, at 35 °C. In which the biohydrogen yield was 3.231 mmol H2/g dry biomass without any pretreatments of the biomass. A freshwater microalga Oscillatroia sp was used for upgrading of the produced biohydrogen. It sequestrated 97 and 99% % of CO2 from the gas mixture when it was cultivated in BG11 and BG11-N media, respectively After upgrading process, the residual microalgal cells exhibited 0.21mg/mL of biomass yield,high content of chlorophyll-a (4.8 µg/mL) and carotenoid (11.1 µg/mL). In addition to Oscillatroia sp residual biomass showed a lipid yield (7.5–8.7%) on the tested media. Conclusion Bacillus coagulans AH1 is a promising tool for biohydrogen production avoiding the drawbacks of biomass pretreatment. Oscillatroia sp is encouraged as a potent tool for upgrading and purification of biohydrogen. These findings led to the development of a multiproduct biorefinery with zero waste that is more economically sustainable. Graphical Abstracthttps://doi.org/10.1186/s12934-023-02193-0BacillusBiohydrogenCO2 sequestrationFermentationMicroalgaeUpgrading |
| spellingShingle | Eman S. E. Aldaby Aya H. A. Mahmoud Haitham M. El-Bery Maysa M. Ali Ahmed A. Shoreit Asmaa M. M. Mawad Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass Microbial Cell Factories Bacillus Biohydrogen CO2 sequestration Fermentation Microalgae Upgrading |
| title | Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass |
| title_full | Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass |
| title_fullStr | Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass |
| title_full_unstemmed | Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass |
| title_short | Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass |
| title_sort | microalgal upgrading of the fermentative biohydrogen produced from bacillus coagulans via non pretreated plant biomass |
| topic | Bacillus Biohydrogen CO2 sequestration Fermentation Microalgae Upgrading |
| url | https://doi.org/10.1186/s12934-023-02193-0 |
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