Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies
Nanomaterials have unique physicochemical properties compared to their bulk counterparts. Besides, biologically synthesized nanoparticles (NPs) have proven superior to other methods. This work aimed to biosynthesize zinc oxide (ZnO) NPs using an aqueous extract of Lepidium sativum seed. The obtained...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2023-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2023/4166128 |
| _version_ | 1797670367259525120 |
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| author | Adnan Alnehia Annas Al-Sharabi Abdel-Basit Al-Odayni A. H. Al-Hammadi Fares H. AL-Ostoot Waseem Sharaf Saeed Naaser A. Y. Abduh Ali Alrahlah |
| author_facet | Adnan Alnehia Annas Al-Sharabi Abdel-Basit Al-Odayni A. H. Al-Hammadi Fares H. AL-Ostoot Waseem Sharaf Saeed Naaser A. Y. Abduh Ali Alrahlah |
| author_sort | Adnan Alnehia |
| collection | DOAJ |
| description | Nanomaterials have unique physicochemical properties compared to their bulk counterparts. Besides, biologically synthesized nanoparticles (NPs) have proven superior to other methods. This work aimed to biosynthesize zinc oxide (ZnO) NPs using an aqueous extract of Lepidium sativum seed. The obtained ZnO NPs were characterized by X-ray diffraction, scanning electron microscopy, Fourier transform infrared, and ultraviolet-visible spectroscopy. The in vitro antibacterial activity of ZnO NPs against Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was assessed using the disk diffusion technique. The hemolytic impact was quantified spectrophotometrically. The results indicated a 24.2 nm crystallite size, a hexagonal structure phase, and a 3.48 eV optical bandgap. Antibacterial studies revealed a dose-dependent response with comparable activity to the standard drug (gentamicin) and higher activity against S. aureus than E. coli, e.g., the zone of inhibition at 120 mg/mL was 23 ± 1.25 and 16 ± 1.00 mm, respectively. The hemolysis assay showed no potential harm due to ZnO NPs toward red blood cells if utilized in low doses. As a result, it could be concluded that the reported biogenic method for synthesizing ZnO NPs is promising, resulting in hemocompatible NPs and comparable bactericidal agents. |
| first_indexed | 2024-03-11T20:58:41Z |
| format | Article |
| id | doaj.art-62319451761c4b43b4f305d550528245 |
| institution | Directory Open Access Journal |
| issn | 1687-479X |
| language | English |
| last_indexed | 2024-03-11T20:58:41Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj.art-62319451761c4b43b4f305d5505282452023-09-30T00:00:01ZengHindawi LimitedBioinorganic Chemistry and Applications1687-479X2023-01-01202310.1155/2023/4166128Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial StudiesAdnan Alnehia0Annas Al-Sharabi1Abdel-Basit Al-Odayni2A. H. Al-Hammadi3Fares H. AL-Ostoot4Waseem Sharaf Saeed5Naaser A. Y. Abduh6Ali Alrahlah7Department of PhysicsDepartment of PhysicsEngineer Abdullah Bugshan Research Chair for Dental and Oral RehabilitationDepartment of PhysicsDepartment of BiochemistryEngineer Abdullah Bugshan Research Chair for Dental and Oral RehabilitationDepartment of ChemistryEngineer Abdullah Bugshan Research Chair for Dental and Oral RehabilitationNanomaterials have unique physicochemical properties compared to their bulk counterparts. Besides, biologically synthesized nanoparticles (NPs) have proven superior to other methods. This work aimed to biosynthesize zinc oxide (ZnO) NPs using an aqueous extract of Lepidium sativum seed. The obtained ZnO NPs were characterized by X-ray diffraction, scanning electron microscopy, Fourier transform infrared, and ultraviolet-visible spectroscopy. The in vitro antibacterial activity of ZnO NPs against Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was assessed using the disk diffusion technique. The hemolytic impact was quantified spectrophotometrically. The results indicated a 24.2 nm crystallite size, a hexagonal structure phase, and a 3.48 eV optical bandgap. Antibacterial studies revealed a dose-dependent response with comparable activity to the standard drug (gentamicin) and higher activity against S. aureus than E. coli, e.g., the zone of inhibition at 120 mg/mL was 23 ± 1.25 and 16 ± 1.00 mm, respectively. The hemolysis assay showed no potential harm due to ZnO NPs toward red blood cells if utilized in low doses. As a result, it could be concluded that the reported biogenic method for synthesizing ZnO NPs is promising, resulting in hemocompatible NPs and comparable bactericidal agents.http://dx.doi.org/10.1155/2023/4166128 |
| spellingShingle | Adnan Alnehia Annas Al-Sharabi Abdel-Basit Al-Odayni A. H. Al-Hammadi Fares H. AL-Ostoot Waseem Sharaf Saeed Naaser A. Y. Abduh Ali Alrahlah Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies Bioinorganic Chemistry and Applications |
| title | Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies |
| title_full | Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies |
| title_fullStr | Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies |
| title_full_unstemmed | Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies |
| title_short | Lepidium sativum Seed Extract-Mediated Synthesis of Zinc Oxide Nanoparticles: Structural, Morphological, Optical, Hemolysis, and Antibacterial Studies |
| title_sort | lepidium sativum seed extract mediated synthesis of zinc oxide nanoparticles structural morphological optical hemolysis and antibacterial studies |
| url | http://dx.doi.org/10.1155/2023/4166128 |
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