Monitoring Fungal Communities With the Global Spore Sampling Project
The kingdom Fungi is a megadiverse group represented in all ecosystem types. The global diversity and distribution of fungal taxa are poorly known, in part due to the limitations related to traditional fruit-body survey methods. These previous hurdles are now being overcome by rapidly developing DNA...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fevo.2019.00511/full |
| _version_ | 1818037102017773568 |
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| author | Otso Ovaskainen Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Bess Hardwick Juha-Matti Pitkänen Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Niels Martin Schmidt Sebastian Seibold Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Tomas Roslin Natalia V. Ivanova |
| author_facet | Otso Ovaskainen Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Bess Hardwick Juha-Matti Pitkänen Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Niels Martin Schmidt Sebastian Seibold Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Tomas Roslin Natalia V. Ivanova |
| author_sort | Otso Ovaskainen |
| collection | DOAJ |
| description | The kingdom Fungi is a megadiverse group represented in all ecosystem types. The global diversity and distribution of fungal taxa are poorly known, in part due to the limitations related to traditional fruit-body survey methods. These previous hurdles are now being overcome by rapidly developing DNA-based surveys. Past fungal DNA surveys have predominantly examined soil samples, which capture high species diversity but represent only the local soil community. Recent work has shown that DNA samples collected from the air with cyclone samplers provide information on fungal diversity at the scale of some tens of kilometers around the sampling location. To test the feasibility of air sampling for investigating global patterns of fungal diversity, we established a new initiative called the Global Spore Sampling Project (GSSP). The GSSP currently involves 50 sampling locations distributed on all continents, with each location collecting two 24-h samples per week. Here we describe the GSSP methodology, including the sampling, DNA extraction and sequencing protocols, and the bioinformatics pipeline. We further report results based on 75 pilot samples from five locations, of which three in Europe, one in Australia, and one in Greenland. The results show highly consistent patterns, suggesting that GSSP holds much promise for systematic global fungal monitoring. The GSSP provides highly standardized sampling across space and time, enabling much-improved estimation of total fungal diversity, the global distribution of different fungal groups, fungal fruiting phenology, and the extent of long-distance dispersal in fungi. |
| first_indexed | 2024-12-10T07:21:30Z |
| format | Article |
| id | doaj.art-6232c510cbcd4b489e439560e7fc7b1a |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-12-10T07:21:30Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-6232c510cbcd4b489e439560e7fc7b1a2022-12-22T01:57:48ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2020-01-01710.3389/fevo.2019.00511484146Monitoring Fungal Communities With the Global Spore Sampling ProjectOtso Ovaskainen0Otso Ovaskainen1Nerea Abrego2Panu Somervuo3Isabella Palorinne4Bess Hardwick5Bess Hardwick6Juha-Matti Pitkänen7Juha-Matti Pitkänen8Nigel R. Andrew9Pascal A. Niklaus10Niels Martin Schmidt11Niels Martin Schmidt12Sebastian Seibold13Sebastian Seibold14Juliane Vogt15Evgeny V. Zakharov16Paul D. N. Hebert17Tomas Roslin18Tomas Roslin19Natalia V. Ivanova20Organismal and Evolutionary Biology Research Programme, University of Helsinki, Helsinki, FinlandDepartment of Biology, Centre for Biodiversity Dynamics, Norwegian University of Science and Technology, Trondheim, NorwaySpatial Foodweb Ecology Group, Department of Agricultural Sciences, University of Helsinki, Helsinki, FinlandOrganismal and Evolutionary Biology Research Programme, University of Helsinki, Helsinki, FinlandSpatial Foodweb Ecology Group, Department of Agricultural Sciences, University of Helsinki, Helsinki, FinlandOrganismal and Evolutionary Biology Research Programme, University of Helsinki, Helsinki, FinlandSpatial Foodweb Ecology Group, Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, SwedenSpatial Foodweb Ecology Group, Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, SwedenForest Health and Biodiversity, Natural Resources Institute Finland (LUKE), Helsinki, FinlandZoology, University of New England, Armidale, NSW, AustraliaDepartment of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, SwitzerlandDepartment of Bioscience, Aarhus University, Roskilde, DenmarkArctic Research Centre, Aarhus University, Aarhus, Denmark0Terrestrial Ecology Research Group, Department of Ecology and Ecosystem Management, Technical University of Munich, Freising, Germany1Field Station Fabrikschleichach, Department of Animal Ecology and Tropical Biology, Julius-Maximilians-University Würzburg, Rauhenebrach, Germany0Terrestrial Ecology Research Group, Department of Ecology and Ecosystem Management, Technical University of Munich, Freising, Germany2Centre for Biodiversity Genomics & Department of Integrative Biology, University of Guelph, Guelph, ON, Canada2Centre for Biodiversity Genomics & Department of Integrative Biology, University of Guelph, Guelph, ON, CanadaSpatial Foodweb Ecology Group, Department of Agricultural Sciences, University of Helsinki, Helsinki, FinlandSpatial Foodweb Ecology Group, Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden2Centre for Biodiversity Genomics & Department of Integrative Biology, University of Guelph, Guelph, ON, CanadaThe kingdom Fungi is a megadiverse group represented in all ecosystem types. The global diversity and distribution of fungal taxa are poorly known, in part due to the limitations related to traditional fruit-body survey methods. These previous hurdles are now being overcome by rapidly developing DNA-based surveys. Past fungal DNA surveys have predominantly examined soil samples, which capture high species diversity but represent only the local soil community. Recent work has shown that DNA samples collected from the air with cyclone samplers provide information on fungal diversity at the scale of some tens of kilometers around the sampling location. To test the feasibility of air sampling for investigating global patterns of fungal diversity, we established a new initiative called the Global Spore Sampling Project (GSSP). The GSSP currently involves 50 sampling locations distributed on all continents, with each location collecting two 24-h samples per week. Here we describe the GSSP methodology, including the sampling, DNA extraction and sequencing protocols, and the bioinformatics pipeline. We further report results based on 75 pilot samples from five locations, of which three in Europe, one in Australia, and one in Greenland. The results show highly consistent patterns, suggesting that GSSP holds much promise for systematic global fungal monitoring. The GSSP provides highly standardized sampling across space and time, enabling much-improved estimation of total fungal diversity, the global distribution of different fungal groups, fungal fruiting phenology, and the extent of long-distance dispersal in fungi.https://www.frontiersin.org/article/10.3389/fevo.2019.00511/fullbiomonitoringcyclone samplerenvironmental DNAfungiglobal diversity |
| spellingShingle | Otso Ovaskainen Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Bess Hardwick Juha-Matti Pitkänen Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Niels Martin Schmidt Sebastian Seibold Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Tomas Roslin Natalia V. Ivanova Monitoring Fungal Communities With the Global Spore Sampling Project Frontiers in Ecology and Evolution biomonitoring cyclone sampler environmental DNA fungi global diversity |
| title | Monitoring Fungal Communities With the Global Spore Sampling Project |
| title_full | Monitoring Fungal Communities With the Global Spore Sampling Project |
| title_fullStr | Monitoring Fungal Communities With the Global Spore Sampling Project |
| title_full_unstemmed | Monitoring Fungal Communities With the Global Spore Sampling Project |
| title_short | Monitoring Fungal Communities With the Global Spore Sampling Project |
| title_sort | monitoring fungal communities with the global spore sampling project |
| topic | biomonitoring cyclone sampler environmental DNA fungi global diversity |
| url | https://www.frontiersin.org/article/10.3389/fevo.2019.00511/full |
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