Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase
Abstract The extracellular 373‐kDa PehA heme peroxidase of Pseudomonas putida KT2440 has two enzymatic domains which depend on heme cofactor for their peroxidase activity. A null pehA mutant was generated to examine the impact of PehA in rhizosphere colonization competence and the induction of plant...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-10-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14123 |
| _version_ | 1798000186048380928 |
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| author | Saray Santamaría‐Hernando Lieselotte De Bruyne Monica Höfte María‐Isabel Ramos‐González |
| author_facet | Saray Santamaría‐Hernando Lieselotte De Bruyne Monica Höfte María‐Isabel Ramos‐González |
| author_sort | Saray Santamaría‐Hernando |
| collection | DOAJ |
| description | Abstract The extracellular 373‐kDa PehA heme peroxidase of Pseudomonas putida KT2440 has two enzymatic domains which depend on heme cofactor for their peroxidase activity. A null pehA mutant was generated to examine the impact of PehA in rhizosphere colonization competence and the induction of plant systemic resistance (ISR). This mutant was not markedly hampered in colonization efficiency. However, increase in pehA dosage enhanced colonization fitness about 30 fold in the root and 900 fold in the root apex. In vitro assays with purified His‐tagged enzymatic domains of PehA indicated that heme‐dependent peroxidase activity was required for the enhancement of root tip colonization. Evaluation of live/dead cells confirmed that overexpression of pehA had a positive effect on bacterial cell viability. Following root colonization of rice plants by KT2440 strain, the incidence of rice blast caused by Magnaporthe oryzae was reduced by 65% and the severity of this disease was also diminished in comparison to non‐treated plants. An increase in the pehA dosage was also beneficial for the control of rice blast as compared with gene inactivation. The results suggest that PehA helps P. putida to cope with the plant‐imposed oxidative stress leading to enhanced colonization ability and concomitant ISR‐elicitation. |
| first_indexed | 2024-04-11T11:16:09Z |
| format | Article |
| id | doaj.art-6234ca3ff862408d974eb461525a13d2 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-11T11:16:09Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-6234ca3ff862408d974eb461525a13d22022-12-22T04:27:14ZengWileyMicrobial Biotechnology1751-79152022-10-0115102652266610.1111/1751-7915.14123Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidaseSaray Santamaría‐Hernando0Lieselotte De Bruyne1Monica Höfte2María‐Isabel Ramos‐González3Department of Environmental Protection Estación Experimental de Zaidín‐Consejo Superior de Investigaciones Científicas (CSIC) Granada SpainLaboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering Ghent University Ghent BelgiumLaboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering Ghent University Ghent BelgiumDepartment of Environmental Protection Estación Experimental de Zaidín‐Consejo Superior de Investigaciones Científicas (CSIC) Granada SpainAbstract The extracellular 373‐kDa PehA heme peroxidase of Pseudomonas putida KT2440 has two enzymatic domains which depend on heme cofactor for their peroxidase activity. A null pehA mutant was generated to examine the impact of PehA in rhizosphere colonization competence and the induction of plant systemic resistance (ISR). This mutant was not markedly hampered in colonization efficiency. However, increase in pehA dosage enhanced colonization fitness about 30 fold in the root and 900 fold in the root apex. In vitro assays with purified His‐tagged enzymatic domains of PehA indicated that heme‐dependent peroxidase activity was required for the enhancement of root tip colonization. Evaluation of live/dead cells confirmed that overexpression of pehA had a positive effect on bacterial cell viability. Following root colonization of rice plants by KT2440 strain, the incidence of rice blast caused by Magnaporthe oryzae was reduced by 65% and the severity of this disease was also diminished in comparison to non‐treated plants. An increase in the pehA dosage was also beneficial for the control of rice blast as compared with gene inactivation. The results suggest that PehA helps P. putida to cope with the plant‐imposed oxidative stress leading to enhanced colonization ability and concomitant ISR‐elicitation.https://doi.org/10.1111/1751-7915.14123 |
| spellingShingle | Saray Santamaría‐Hernando Lieselotte De Bruyne Monica Höfte María‐Isabel Ramos‐González Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase Microbial Biotechnology |
| title | Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase |
| title_full | Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase |
| title_fullStr | Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase |
| title_full_unstemmed | Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase |
| title_short | Improvement of fitness and biocontrol properties of Pseudomonas putida via an extracellular heme peroxidase |
| title_sort | improvement of fitness and biocontrol properties of pseudomonas putida via an extracellular heme peroxidase |
| url | https://doi.org/10.1111/1751-7915.14123 |
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