Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala
Chewing the habit of blended pan masala containing areca nut with or without tobacco is a common practice in the Indian subcontinent. Arecoline, a pyridine alkaloid presence in areca nut alarmed for oral carcinogenesis and strictly prohibited in the western world. However, in India using blended pan...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2015;volume=6;issue=4;spage=195;epage=199;aulast=Adhikari |
| _version_ | 1818694118732201984 |
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| author | Anjan Adhikari Alok Kumar Hazra Tapas Kumar Sur |
| author_facet | Anjan Adhikari Alok Kumar Hazra Tapas Kumar Sur |
| author_sort | Anjan Adhikari |
| collection | DOAJ |
| description | Chewing the habit of blended pan masala containing areca nut with or without tobacco is a common practice in the Indian subcontinent. Arecoline, a pyridine alkaloid presence in areca nut alarmed for oral carcinogenesis and strictly prohibited in the western world. However, in India using blended pan masala is very popular among young and old individuals. In this context, we aimed to detect arecoline in Indian blended nontobacco pan masala sold in Kolkata using a simple densitometric high-performance thin-layer chromatographic (HPTLC) method and for alarming their use in common people. Eleven popularly Indian blended nontobacco pan masala were collected from the territory of Kolkata and isolated arecoline, following solvent extraction method derived for pyridine alkaloid. The quantitative analysis of arecoline was measured using automated software-based HPTLC instruments and validated the method according to International Conference on Harmonization guidelines. Arecoline was detected in all 11 blended nontobacco pan masala samples in a range of minimum 130 to maximum 415 μg/g dry samples. Arecoline is hazardous carcinogenic compound, so the use of Indian blended nontobacco pan masala should be restricted. Further, the method was found suitable for routine quantitative analysis of arecoline in areca nut containing substances. |
| first_indexed | 2024-12-17T13:24:30Z |
| format | Article |
| id | doaj.art-62365ace03ca4af18616db642d652d59 |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-17T13:24:30Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-62365ace03ca4af18616db642d652d592022-12-21T21:46:46ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942015-01-016419519910.4103/2231-4040.165022Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masalaAnjan AdhikariAlok Kumar HazraTapas Kumar SurChewing the habit of blended pan masala containing areca nut with or without tobacco is a common practice in the Indian subcontinent. Arecoline, a pyridine alkaloid presence in areca nut alarmed for oral carcinogenesis and strictly prohibited in the western world. However, in India using blended pan masala is very popular among young and old individuals. In this context, we aimed to detect arecoline in Indian blended nontobacco pan masala sold in Kolkata using a simple densitometric high-performance thin-layer chromatographic (HPTLC) method and for alarming their use in common people. Eleven popularly Indian blended nontobacco pan masala were collected from the territory of Kolkata and isolated arecoline, following solvent extraction method derived for pyridine alkaloid. The quantitative analysis of arecoline was measured using automated software-based HPTLC instruments and validated the method according to International Conference on Harmonization guidelines. Arecoline was detected in all 11 blended nontobacco pan masala samples in a range of minimum 130 to maximum 415 μg/g dry samples. Arecoline is hazardous carcinogenic compound, so the use of Indian blended nontobacco pan masala should be restricted. Further, the method was found suitable for routine quantitative analysis of arecoline in areca nut containing substances.http://www.japtr.org/article.asp?issn=2231-4040;year=2015;volume=6;issue=4;spage=195;epage=199;aulast=AdhikariAlkaloidareca nutarecolinehigh-performance thin-layer chromatographicpan masala |
| spellingShingle | Anjan Adhikari Alok Kumar Hazra Tapas Kumar Sur Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala Journal of Advanced Pharmaceutical Technology & Research Alkaloid areca nut arecoline high-performance thin-layer chromatographic pan masala |
| title | Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala |
| title_full | Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala |
| title_fullStr | Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala |
| title_full_unstemmed | Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala |
| title_short | Detection of arecoline by simple high-performance thin-layer chromatographic method in Indian nontobacco pan masala |
| title_sort | detection of arecoline by simple high performance thin layer chromatographic method in indian nontobacco pan masala |
| topic | Alkaloid areca nut arecoline high-performance thin-layer chromatographic pan masala |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2015;volume=6;issue=4;spage=195;epage=199;aulast=Adhikari |
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