Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015
objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extraction and characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocin activity. Results showed that the bacteriocin activity increased during growth a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2006-12-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/7566 |
| _version_ | 1819093085121937408 |
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| author | N. Nofisulastri Zaenal Bachruddin Eni Harmayani |
| author_facet | N. Nofisulastri Zaenal Bachruddin Eni Harmayani |
| author_sort | N. Nofisulastri |
| collection | DOAJ |
| description | objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extraction
and characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocin
activity. Results showed that the bacteriocin activity increased during growth and reached the highest activity during
stationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGE
broth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specific
activity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is still
active by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effective
againts Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonella
thypimurium. The molecular weight of bacteriocin is 4.95 kDa.
Keywords : Bacteriocins, Pediococcus sp NWD 015. |
| first_indexed | 2024-12-21T23:05:54Z |
| format | Article |
| id | doaj.art-6238862648bc47efb02dcd6115180534 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-21T23:05:54Z |
| publishDate | 2006-12-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-6238862648bc47efb02dcd61151805342022-12-21T18:47:10ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412006-12-0111210.22146/ijbiotech.75666367Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015N. NofisulastriZaenal BachruddinEni Harmayaniobjectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extraction and characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocin activity. Results showed that the bacteriocin activity increased during growth and reached the highest activity during stationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGE broth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specific activity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is still active by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effective againts Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonella thypimurium. The molecular weight of bacteriocin is 4.95 kDa. Keywords : Bacteriocins, Pediococcus sp NWD 015.https://jurnal.ugm.ac.id/ijbiotech/article/view/7566 |
| spellingShingle | N. Nofisulastri Zaenal Bachruddin Eni Harmayani Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 Indonesian Journal of Biotechnology |
| title | Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 |
| title_full | Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 |
| title_fullStr | Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 |
| title_full_unstemmed | Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 |
| title_short | Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 |
| title_sort | production and extraction of antibacterial bacteriocin from pediococcus sp nwd 015 |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/7566 |
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