Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm
Bioactive materials were developed with the ability to release fluoride and provide some antimicrobial potential, to be widely used in dentistry today. However, few scientific studies have evaluated the antimicrobial activity of bioactive surface pre-reacted glass (S-PRG) coatings (PRG Barrier Coat,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/6/1727 |
| _version_ | 1827735955936641024 |
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| author | Caio Junji Tanaka José Augusto Rodrigues João Marcos Spessoto Pingueiro Tatiane Tiemi Macedo Magda Feres Jamil Awad Shibli Bruno Bueno-Silva |
| author_facet | Caio Junji Tanaka José Augusto Rodrigues João Marcos Spessoto Pingueiro Tatiane Tiemi Macedo Magda Feres Jamil Awad Shibli Bruno Bueno-Silva |
| author_sort | Caio Junji Tanaka |
| collection | DOAJ |
| description | Bioactive materials were developed with the ability to release fluoride and provide some antimicrobial potential, to be widely used in dentistry today. However, few scientific studies have evaluated the antimicrobial activity of bioactive surface pre-reacted glass (S-PRG) coatings (PRG Barrier Coat, Shofu, Kyoto, Japan) on periodontopathogenic biofilms. This study evaluated the antibacterial activity of S-PRG fillers on the microbial profile of multispecies subgingival biofilms. A Calgary Biofilm Device (CBD) was used to grow a 33-species biofilm related to periodontitis for 7 days. The S-PRG coating was applied on CBD pins from the test group and photo-activated (PRG Barrier Coat, Shofu), while the control group received no coating. Seven days after treatment, the total bacterial counts, metabolic activity, and microbial profile of the biofilms were observed using a colorimetric assay and DNA–DNA hybridization. Statistical analyses were applied; namely, the Mann–Whitney, Kruskal–Wallis, and Dunn’s post hoc tests. The bacterial activity of the test group was reduced by 25.7% compared with that of the control group. A statistically significant reduction was observed for the counts of 15 species: <i>A. naeslundii</i>, <i>A. odontolyticus</i>, <i>V. parvula</i>, <i>C. ochracea</i>, <i>C. sputigena</i>, <i>E. corrodens</i>, <i>C. gracilis</i>, <i>F. nucleatum polymorphum</i>, <i>F. nucleatum vincentii</i>, <i>F. periodonticum</i>, <i>P. intermedia</i>, <i>P. gingivalis</i>, <i>G. morbillorum</i>, <i>S. anginosus</i>, and <i>S. noxia</i> (<i>p</i> ≤ 0.05). The bioactive coating containing S-PRG modified the composition of the subgingival biofilm in vitro, thereby decreasing colonization by pathogens. |
| first_indexed | 2024-03-11T02:01:58Z |
| format | Article |
| id | doaj.art-6238a3e20b794ffd837f9e74b3d69e6b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T02:01:58Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-6238a3e20b794ffd837f9e74b3d69e6b2023-11-18T12:05:39ZengMDPI AGPharmaceutics1999-49232023-06-01156172710.3390/pharmaceutics15061727Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival BiofilmCaio Junji Tanaka0José Augusto Rodrigues1João Marcos Spessoto Pingueiro2Tatiane Tiemi Macedo3Magda Feres4Jamil Awad Shibli5Bruno Bueno-Silva6School of Dentistry, Mogi das Cruzes University, Mogi das Cruzes 08780-911, Sao Paulo, BrazilDental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, BrazilDental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, BrazilDental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, BrazilDental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, BrazilDental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, BrazilDental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, BrazilBioactive materials were developed with the ability to release fluoride and provide some antimicrobial potential, to be widely used in dentistry today. However, few scientific studies have evaluated the antimicrobial activity of bioactive surface pre-reacted glass (S-PRG) coatings (PRG Barrier Coat, Shofu, Kyoto, Japan) on periodontopathogenic biofilms. This study evaluated the antibacterial activity of S-PRG fillers on the microbial profile of multispecies subgingival biofilms. A Calgary Biofilm Device (CBD) was used to grow a 33-species biofilm related to periodontitis for 7 days. The S-PRG coating was applied on CBD pins from the test group and photo-activated (PRG Barrier Coat, Shofu), while the control group received no coating. Seven days after treatment, the total bacterial counts, metabolic activity, and microbial profile of the biofilms were observed using a colorimetric assay and DNA–DNA hybridization. Statistical analyses were applied; namely, the Mann–Whitney, Kruskal–Wallis, and Dunn’s post hoc tests. The bacterial activity of the test group was reduced by 25.7% compared with that of the control group. A statistically significant reduction was observed for the counts of 15 species: <i>A. naeslundii</i>, <i>A. odontolyticus</i>, <i>V. parvula</i>, <i>C. ochracea</i>, <i>C. sputigena</i>, <i>E. corrodens</i>, <i>C. gracilis</i>, <i>F. nucleatum polymorphum</i>, <i>F. nucleatum vincentii</i>, <i>F. periodonticum</i>, <i>P. intermedia</i>, <i>P. gingivalis</i>, <i>G. morbillorum</i>, <i>S. anginosus</i>, and <i>S. noxia</i> (<i>p</i> ≤ 0.05). The bioactive coating containing S-PRG modified the composition of the subgingival biofilm in vitro, thereby decreasing colonization by pathogens.https://www.mdpi.com/1999-4923/15/6/1727PRG fillerresin coatingvarnishbioactivitybiofilmsantimicrobial activity |
| spellingShingle | Caio Junji Tanaka José Augusto Rodrigues João Marcos Spessoto Pingueiro Tatiane Tiemi Macedo Magda Feres Jamil Awad Shibli Bruno Bueno-Silva Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm Pharmaceutics PRG filler resin coating varnish bioactivity biofilms antimicrobial activity |
| title | Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm |
| title_full | Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm |
| title_fullStr | Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm |
| title_full_unstemmed | Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm |
| title_short | Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm |
| title_sort | antibacterial activity of a bioactive tooth coating material containing surface pre reacted glass in a complex multispecies subgingival biofilm |
| topic | PRG filler resin coating varnish bioactivity biofilms antimicrobial activity |
| url | https://www.mdpi.com/1999-4923/15/6/1727 |
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