Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump
Sickle cell disease is a red blood cells disorder, which affects millions of people in the world. One of its clinical manifestations is cation fluxes. Although the antisickling potentials of hydroxyurea (HU), Cajanus cajan seed (CCS) and Zanthoxylum zanthoxyloides (ZZL) have been reported, their int...
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| Format: | Article |
| Language: | English |
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Plovdiv University Press
2017-08-01
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| Series: | Journal of BioScience and Biotechnology |
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| Online Access: | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/110 |
| _version_ | 1811284296767373312 |
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| author | Ismaila O. Nurain Clement O. Bewaji |
| author_facet | Ismaila O. Nurain Clement O. Bewaji |
| author_sort | Ismaila O. Nurain |
| collection | DOAJ |
| description | Sickle cell disease is a red blood cells disorder, which affects millions of people in the world. One of its clinical manifestations is cation fluxes. Although the antisickling potentials of hydroxyurea (HU), Cajanus cajan seed (CCS) and Zanthoxylum zanthoxyloides (ZZL) have been reported, their interactions with human erythrocytes calcium pump have not been elucidated. The calcium pumps catalyses the hydrolysis of adenosine triphosphate and transport calcium out of the cell thereby maintaining low calcium concentration required by the cells.
Investigation of the interaction of HU, CCS and ZZL with calcium adenosine triphosphatase (Ca2+-ATPase) in human sickle (HbSS) and normal (HbAA) erythrocyte membranes was carried out using in vitro approach.
The results indicated that the catalytic efficiency of Ca2+-ATPase in the absence of antisickling agents in HbAA (36.34 mM-1) was higher than in HbSS (33.23 mM-1). Thus, in the presence of HU, the catalytic efficiency in HbAA and HbSS was 51.59 (mM-1) and 41.14 (mM-1) respectively. In the presence of C. cajan seed in HbAA and HbSS, the efficiency of the enzyme was 5098.33 (mM-1) and 537.083 (mM-1) respectively. However, there were no changes in the catalytic efficiency of the enzyme in the presence of Z. zanthoxyloides leaf in both genotypes.
The interaction of the antisickling agents with Ca2+-ATPase indicates a mechanism of antisickling of the agents. This suggests that the agents prevent the red blood cells dehydration by increasing the efficiency of the pump. The plants could be explored in the development of antisickling drugs. |
| first_indexed | 2024-04-13T02:26:32Z |
| format | Article |
| id | doaj.art-62395f7e343c465980413c1fcaf9a682 |
| institution | Directory Open Access Journal |
| issn | 1314-6238 1314-6246 |
| language | English |
| last_indexed | 2024-04-13T02:26:32Z |
| publishDate | 2017-08-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj.art-62395f7e343c465980413c1fcaf9a6822022-12-22T03:06:45ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462017-08-0162111117Interaction of Some Antisickling Agents with Human Erythrocytes Calcium PumpIsmaila O. Nurain0Clement O. Bewaji1Department of Biochemistry, College of Pure and Applied Sciences, Kwara State University Malete, P.M.B. 1530 Ilorin, Kwara State, Nigeria.Biochemistry Department, Faculty of Science, University of Ilorin, Ilorin, Kwara State, Nigeria.Sickle cell disease is a red blood cells disorder, which affects millions of people in the world. One of its clinical manifestations is cation fluxes. Although the antisickling potentials of hydroxyurea (HU), Cajanus cajan seed (CCS) and Zanthoxylum zanthoxyloides (ZZL) have been reported, their interactions with human erythrocytes calcium pump have not been elucidated. The calcium pumps catalyses the hydrolysis of adenosine triphosphate and transport calcium out of the cell thereby maintaining low calcium concentration required by the cells. Investigation of the interaction of HU, CCS and ZZL with calcium adenosine triphosphatase (Ca2+-ATPase) in human sickle (HbSS) and normal (HbAA) erythrocyte membranes was carried out using in vitro approach. The results indicated that the catalytic efficiency of Ca2+-ATPase in the absence of antisickling agents in HbAA (36.34 mM-1) was higher than in HbSS (33.23 mM-1). Thus, in the presence of HU, the catalytic efficiency in HbAA and HbSS was 51.59 (mM-1) and 41.14 (mM-1) respectively. In the presence of C. cajan seed in HbAA and HbSS, the efficiency of the enzyme was 5098.33 (mM-1) and 537.083 (mM-1) respectively. However, there were no changes in the catalytic efficiency of the enzyme in the presence of Z. zanthoxyloides leaf in both genotypes. The interaction of the antisickling agents with Ca2+-ATPase indicates a mechanism of antisickling of the agents. This suggests that the agents prevent the red blood cells dehydration by increasing the efficiency of the pump. The plants could be explored in the development of antisickling drugs.https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/110Sickle Cell DiseaseAntisickling agentsCalcium Pump |
| spellingShingle | Ismaila O. Nurain Clement O. Bewaji Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump Journal of BioScience and Biotechnology Sickle Cell Disease Antisickling agents Calcium Pump |
| title | Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump |
| title_full | Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump |
| title_fullStr | Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump |
| title_full_unstemmed | Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump |
| title_short | Interaction of Some Antisickling Agents with Human Erythrocytes Calcium Pump |
| title_sort | interaction of some antisickling agents with human erythrocytes calcium pump |
| topic | Sickle Cell Disease Antisickling agents Calcium Pump |
| url | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/110 |
| work_keys_str_mv | AT ismailaonurain interactionofsomeantisicklingagentswithhumanerythrocytescalciumpump AT clementobewaji interactionofsomeantisicklingagentswithhumanerythrocytescalciumpump |