Antioxidant Activities and Total Phenolics of Acacia Honey
The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity usin...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2009-06-01
|
| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0053_antioxidant-activities-and-total-phenolics-of-acacia-honey.php |
| _version_ | 1797899814674890752 |
|---|---|
| author | M. Krpan K. Marković G. Šarić B. Skoko M. Hruškar N. Vahčić |
| author_facet | M. Krpan K. Marković G. Šarić B. Skoko M. Hruškar N. Vahčić |
| author_sort | M. Krpan |
| collection | DOAJ |
| description | The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey. |
| first_indexed | 2024-04-10T08:34:51Z |
| format | Article |
| id | doaj.art-6239e595a5f74d9699946859c706cf8f |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:34:51Z |
| publishDate | 2009-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-6239e595a5f74d9699946859c706cf8f2023-02-23T03:27:31ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172009-06-0127Special Issue 1S245S24710.17221/1112-CJFScjf-200910-0053Antioxidant Activities and Total Phenolics of Acacia HoneyM. KrpanK. Marković0G. Šarić1B. Skoko2M. Hruškar3N. Vahčić4Faculty of Food Technology and Biotechnology, University of Zagreb, 10 000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, 10 000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, 10 000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, 10 000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, 10 000 Zagreb, CroatiaThe antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0053_antioxidant-activities-and-total-phenolics-of-acacia-honey.phpantioxidant activityhoneyphenolicsphysicochemical parameters |
| spellingShingle | M. Krpan K. Marković G. Šarić B. Skoko M. Hruškar N. Vahčić Antioxidant Activities and Total Phenolics of Acacia Honey Czech Journal of Food Sciences antioxidant activity honey phenolics physicochemical parameters |
| title | Antioxidant Activities and Total Phenolics of Acacia Honey |
| title_full | Antioxidant Activities and Total Phenolics of Acacia Honey |
| title_fullStr | Antioxidant Activities and Total Phenolics of Acacia Honey |
| title_full_unstemmed | Antioxidant Activities and Total Phenolics of Acacia Honey |
| title_short | Antioxidant Activities and Total Phenolics of Acacia Honey |
| title_sort | antioxidant activities and total phenolics of acacia honey |
| topic | antioxidant activity honey phenolics physicochemical parameters |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0053_antioxidant-activities-and-total-phenolics-of-acacia-honey.php |
| work_keys_str_mv | AT mkrpan antioxidantactivitiesandtotalphenolicsofacaciahoney AT kmarkovic antioxidantactivitiesandtotalphenolicsofacaciahoney AT gsaric antioxidantactivitiesandtotalphenolicsofacaciahoney AT bskoko antioxidantactivitiesandtotalphenolicsofacaciahoney AT mhruskar antioxidantactivitiesandtotalphenolicsofacaciahoney AT nvahcic antioxidantactivitiesandtotalphenolicsofacaciahoney |