MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4
Previous studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Bioengineered |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21655979.2021.1871817 |
| _version_ | 1798034113695842304 |
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| author | Li Cai Lu Ye Xiaoqing Hu Wenfeng He Debao Zhuang Qi Guo Kuanyong Shu Youkun Jie |
| author_facet | Li Cai Lu Ye Xiaoqing Hu Wenfeng He Debao Zhuang Qi Guo Kuanyong Shu Youkun Jie |
| author_sort | Li Cai |
| collection | DOAJ |
| description | Previous studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330-3p in OC tissues and cell lines was detected using RT-qPCR. The proliferation, migration and invasion of OC cells were detected using CCK-8 assay and transwell assay, respectively. Bioinformatics analysis and luciferase reporter assay were used to analyze the targeted binding site of miR-330-3p and RIPK4. The results showed that miR-330-3p was significantly downregulated in OC tissues and cell lines. Overexpression of miR-330-3p inhibited the proliferation, migration and invasion of OC cells. Mechanistically, a dual-luciferase reported assay showed that RIPK4 is a target gene of miR-330-3p. Furthermore, rescue experiments revealed that miR-330-3p suppressed the proliferation, migration and invasion of OC cells by targeting RIPK4. In summary, our findings indicated that miR-330-3p suppressed the progression of OC by targeting RIPK4. Our results indicated that miR-330-3p/RIPK4 axis might act as a novel therapeutic target for OC treatment. |
| first_indexed | 2024-04-11T20:39:50Z |
| format | Article |
| id | doaj.art-623b0522f5264bd8ba12ea29d3c96c5f |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-04-11T20:39:50Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-623b0522f5264bd8ba12ea29d3c96c5f2022-12-22T04:04:14ZengTaylor & Francis GroupBioengineered2165-59792165-59872021-01-0112144044910.1080/21655979.2021.18718171871817MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4Li Cai0Lu Ye1Xiaoqing Hu2Wenfeng He3Debao Zhuang4Qi Guo5Kuanyong Shu6Youkun Jie7Jiangxi Maternal and Child Health HospitalJiangxi Maternal and Child Health HospitalJiangxi Maternal and Child Health HospitalThe Second Affiliated Hospital of Nanchang UniversityJiangxi Maternal and Child Health HospitalJiangxi Maternal and Child Health HospitalJiangxi Maternal and Child Health HospitalJiangxi Maternal and Child Health HospitalPrevious studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330-3p in OC tissues and cell lines was detected using RT-qPCR. The proliferation, migration and invasion of OC cells were detected using CCK-8 assay and transwell assay, respectively. Bioinformatics analysis and luciferase reporter assay were used to analyze the targeted binding site of miR-330-3p and RIPK4. The results showed that miR-330-3p was significantly downregulated in OC tissues and cell lines. Overexpression of miR-330-3p inhibited the proliferation, migration and invasion of OC cells. Mechanistically, a dual-luciferase reported assay showed that RIPK4 is a target gene of miR-330-3p. Furthermore, rescue experiments revealed that miR-330-3p suppressed the proliferation, migration and invasion of OC cells by targeting RIPK4. In summary, our findings indicated that miR-330-3p suppressed the progression of OC by targeting RIPK4. Our results indicated that miR-330-3p/RIPK4 axis might act as a novel therapeutic target for OC treatment.http://dx.doi.org/10.1080/21655979.2021.1871817mir-330-3pripk4ovarian cancer |
| spellingShingle | Li Cai Lu Ye Xiaoqing Hu Wenfeng He Debao Zhuang Qi Guo Kuanyong Shu Youkun Jie MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4 Bioengineered mir-330-3p ripk4 ovarian cancer |
| title | MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4 |
| title_full | MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4 |
| title_fullStr | MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4 |
| title_full_unstemmed | MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4 |
| title_short | MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4 |
| title_sort | microrna mir 330 3p suppresses the progression of ovarian cancer by targeting ripk4 |
| topic | mir-330-3p ripk4 ovarian cancer |
| url | http://dx.doi.org/10.1080/21655979.2021.1871817 |
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