Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum
L-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithin...
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| Format: | Article |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00440/full |
| _version_ | 1818154769864196096 |
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| author | Xiao-Yu Wu Xiao-Yan Guo Bin Zhang Yan Jiang Bang-Ce Ye |
| author_facet | Xiao-Yu Wu Xiao-Yan Guo Bin Zhang Yan Jiang Bang-Ce Ye |
| author_sort | Xiao-Yu Wu |
| collection | DOAJ |
| description | L-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithine by microbial fermentation is low and rarely meets the market demands. Various strategies have been employed to improve the L-ornithine production titers in the model strain, Corynebacterium glutamicum, which serves as a major indicator for improving the cost-effectiveness of L-ornithine production by microbial fermentation. This review focuses on the development of high L-ornithine-producing strains by metabolic engineering and reviews the recent advances in breeding strategies, such as reducing by-product formation, improving the supplementation of precursor glutamate, releasing negative regulation and negative feedback inhibition, increasing the supply of intracellular cofactors, modulating the central metabolic pathway, enhancing the transport system, and adaptive evolution for improving L-ornithine production. |
| first_indexed | 2024-12-11T14:31:47Z |
| format | Article |
| id | doaj.art-623c77c1f4114608af55f8030ddc7255 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T14:31:47Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-623c77c1f4114608af55f8030ddc72552022-12-22T01:02:23ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-01-01710.3389/fbioe.2019.00440505381Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicumXiao-Yu Wu0Xiao-Yan Guo1Bin Zhang2Yan Jiang3Bang-Ce Ye4Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, ChinaLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, ChinaL-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithine by microbial fermentation is low and rarely meets the market demands. Various strategies have been employed to improve the L-ornithine production titers in the model strain, Corynebacterium glutamicum, which serves as a major indicator for improving the cost-effectiveness of L-ornithine production by microbial fermentation. This review focuses on the development of high L-ornithine-producing strains by metabolic engineering and reviews the recent advances in breeding strategies, such as reducing by-product formation, improving the supplementation of precursor glutamate, releasing negative regulation and negative feedback inhibition, increasing the supply of intracellular cofactors, modulating the central metabolic pathway, enhancing the transport system, and adaptive evolution for improving L-ornithine production.https://www.frontiersin.org/article/10.3389/fbioe.2019.00440/fullL-ornithineCorynebacterium glutamicummetabolic engineeringgenetic engineeringfermentation |
| spellingShingle | Xiao-Yu Wu Xiao-Yan Guo Bin Zhang Yan Jiang Bang-Ce Ye Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum Frontiers in Bioengineering and Biotechnology L-ornithine Corynebacterium glutamicum metabolic engineering genetic engineering fermentation |
| title | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
| title_full | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
| title_fullStr | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
| title_full_unstemmed | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
| title_short | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
| title_sort | recent advances of l ornithine biosynthesis in metabolically engineered corynebacterium glutamicum |
| topic | L-ornithine Corynebacterium glutamicum metabolic engineering genetic engineering fermentation |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00440/full |
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