Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein
Abstract The simultaneous interrogation of both lossy mode (LMR) and surface plasmon (SPR) resonances was herein exploited for the first time to devise a sensor in combination with soft molecularly imprinting of nanoparticles (nanoMIPs), specifically entailed of the selectivity towards the protein b...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38262-y |
| _version_ | 1797778903976116224 |
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| author | Francesco Arcadio Laurent Noël Domenico Del Prete Devid Maniglio Mimimorena Seggio Olivier Soppera Nunzio Cennamo Alessandra Maria Bossi Luigi Zeni |
| author_facet | Francesco Arcadio Laurent Noël Domenico Del Prete Devid Maniglio Mimimorena Seggio Olivier Soppera Nunzio Cennamo Alessandra Maria Bossi Luigi Zeni |
| author_sort | Francesco Arcadio |
| collection | DOAJ |
| description | Abstract The simultaneous interrogation of both lossy mode (LMR) and surface plasmon (SPR) resonances was herein exploited for the first time to devise a sensor in combination with soft molecularly imprinting of nanoparticles (nanoMIPs), specifically entailed of the selectivity towards the protein biomarker human serum transferrin (HTR). Two distinct metal-oxide bilayers, i.e. TiO2–ZrO2 and ZrO2–TiO2, were used in the SPR–LMR sensing platforms. The responses to binding of the target protein HTR of both sensing configurations (TiO2–ZrO2–Au-nanoMIPs, ZrO2–TiO2–Au-nanoMIPs) showed femtomolar HTR detection, LODs of tens of fM and KDapp ~ 30 fM. Selectivity for HTR was demonstrated. The SPR interrogation was more efficient for the ZrO2–TiO2–Au-nanoMIPs configuration (sensitivity at low concentrations, S = 0.108 nm/fM) than for the TiO2–ZrO2–Au-nanoMIPs one (S = 0.061 nm/fM); while LMR was more efficient for TiO2–ZrO2–Au-nanoMIPs (S = 0.396 nm/fM) than for ZrO2–TiO2–Au-nanoMIPs (S = 0.177 nm/fM). The simultaneous resonance monitoring is advantageous for point of care determinations, both in terms of measurement’s redundancy, that enables the cross-control of the measure and the optimization of the detection, by exploiting the individual characteristics of each resonance. |
| first_indexed | 2024-03-12T23:24:14Z |
| format | Article |
| id | doaj.art-623ce8f57a804dcabc3a4978f63d9f97 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T23:24:14Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-623ce8f57a804dcabc3a4978f63d9f972023-07-16T11:15:34ZengNature PortfolioScientific Reports2045-23222023-07-0113111010.1038/s41598-023-38262-ySoft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin proteinFrancesco Arcadio0Laurent Noël1Domenico Del Prete2Devid Maniglio3Mimimorena Seggio4Olivier Soppera5Nunzio Cennamo6Alessandra Maria Bossi7Luigi Zeni8Department of Engineering, University of Campania “L. Vanvitelli”CNRS, IS2M UMR 7361, University of Upper-AlsaceDepartment of Engineering, University of Campania “L. Vanvitelli”Department of Industrial Engineering, University of TrentoDepartment of Biotechnology, University of VeronaCNRS, IS2M UMR 7361, University of Upper-AlsaceDepartment of Engineering, University of Campania “L. Vanvitelli”Department of Biotechnology, University of VeronaDepartment of Engineering, University of Campania “L. Vanvitelli”Abstract The simultaneous interrogation of both lossy mode (LMR) and surface plasmon (SPR) resonances was herein exploited for the first time to devise a sensor in combination with soft molecularly imprinting of nanoparticles (nanoMIPs), specifically entailed of the selectivity towards the protein biomarker human serum transferrin (HTR). Two distinct metal-oxide bilayers, i.e. TiO2–ZrO2 and ZrO2–TiO2, were used in the SPR–LMR sensing platforms. The responses to binding of the target protein HTR of both sensing configurations (TiO2–ZrO2–Au-nanoMIPs, ZrO2–TiO2–Au-nanoMIPs) showed femtomolar HTR detection, LODs of tens of fM and KDapp ~ 30 fM. Selectivity for HTR was demonstrated. The SPR interrogation was more efficient for the ZrO2–TiO2–Au-nanoMIPs configuration (sensitivity at low concentrations, S = 0.108 nm/fM) than for the TiO2–ZrO2–Au-nanoMIPs one (S = 0.061 nm/fM); while LMR was more efficient for TiO2–ZrO2–Au-nanoMIPs (S = 0.396 nm/fM) than for ZrO2–TiO2–Au-nanoMIPs (S = 0.177 nm/fM). The simultaneous resonance monitoring is advantageous for point of care determinations, both in terms of measurement’s redundancy, that enables the cross-control of the measure and the optimization of the detection, by exploiting the individual characteristics of each resonance.https://doi.org/10.1038/s41598-023-38262-y |
| spellingShingle | Francesco Arcadio Laurent Noël Domenico Del Prete Devid Maniglio Mimimorena Seggio Olivier Soppera Nunzio Cennamo Alessandra Maria Bossi Luigi Zeni Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein Scientific Reports |
| title | Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein |
| title_full | Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein |
| title_fullStr | Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein |
| title_full_unstemmed | Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein |
| title_short | Soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi-resonances for femtomolar sensing of serum transferrin protein |
| title_sort | soft molecularly imprinted nanoparticles with simultaneous lossy mode and surface plasmon multi resonances for femtomolar sensing of serum transferrin protein |
| url | https://doi.org/10.1038/s41598-023-38262-y |
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