Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ
This experiment aimed to investigate the role of ERα and ERβ in boron's effect on splenic lymphocyte of rat. After inhibition of ERα and ERβ using MPP and PHTPP, the effects of 0.4 mmol/L boron on the splenic CD4+/CD8+ T-cell ratio, lymphocyte proliferation rate, IFN-γ concentration and PCNA pr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1626809 |
| _version_ | 1818290494774444032 |
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| author | Erhui Jin Yaqiong Pei Ting Liu Man Ren Qianqian Hu Youfang Gu Shenghe Li |
| author_facet | Erhui Jin Yaqiong Pei Ting Liu Man Ren Qianqian Hu Youfang Gu Shenghe Li |
| author_sort | Erhui Jin |
| collection | DOAJ |
| description | This experiment aimed to investigate the role of ERα and ERβ in boron's effect on splenic lymphocyte of rat. After inhibition of ERα and ERβ using MPP and PHTPP, the effects of 0.4 mmol/L boron on the splenic CD4+/CD8+ T-cell ratio, lymphocyte proliferation rate, IFN-γ concentration and PCNA protein expression disappeared. Furthermore, after inhibiting ERβ with PHTPP alone, the decreasing effects of 40 mmol/L boron on the splenic CD8+ T-cell proportion, lymphocyte proliferation rate, the concentrations of IgG and cytokines, as well as the expression of Bcl-2 also disappeared. However, when ERα was inhibited by MPP alone, the effect of 40 mmol/L boron on splenic lymphocyte was unchanged. The results suggest that ERα mainly mediate the effects of low-dose boron (0.4 mmol/L) on splenic T-cell subsets, lymphocyte proliferation and apoptosis, and immune function, while ERβ may mediate the effects of both low-dose and high-dose boron (40 mmol/L) on splenic lymphocytes. |
| first_indexed | 2024-12-13T02:29:04Z |
| format | Article |
| id | doaj.art-623d95f0de5d4bdbbb0be417144d32f8 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-13T02:29:04Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-623d95f0de5d4bdbbb0be417144d32f82022-12-22T00:02:33ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-0130174376110.1080/09540105.2019.16268091626809Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβErhui Jin0Yaqiong Pei1Ting Liu2Man Ren3Qianqian Hu4Youfang Gu5Shenghe Li6Anhui Science and Technology UniversityAnhui Science and Technology UniversityAnhui Science and Technology UniversityAnhui Science and Technology UniversityAnhui Science and Technology UniversityAnhui Science and Technology UniversityAnhui Science and Technology UniversityThis experiment aimed to investigate the role of ERα and ERβ in boron's effect on splenic lymphocyte of rat. After inhibition of ERα and ERβ using MPP and PHTPP, the effects of 0.4 mmol/L boron on the splenic CD4+/CD8+ T-cell ratio, lymphocyte proliferation rate, IFN-γ concentration and PCNA protein expression disappeared. Furthermore, after inhibiting ERβ with PHTPP alone, the decreasing effects of 40 mmol/L boron on the splenic CD8+ T-cell proportion, lymphocyte proliferation rate, the concentrations of IgG and cytokines, as well as the expression of Bcl-2 also disappeared. However, when ERα was inhibited by MPP alone, the effect of 40 mmol/L boron on splenic lymphocyte was unchanged. The results suggest that ERα mainly mediate the effects of low-dose boron (0.4 mmol/L) on splenic T-cell subsets, lymphocyte proliferation and apoptosis, and immune function, while ERβ may mediate the effects of both low-dose and high-dose boron (40 mmol/L) on splenic lymphocytes.http://dx.doi.org/10.1080/09540105.2019.1626809estrogen receptorboronsplenic lymphocytest cell subsetcytokinecell proliferation and apoptosis |
| spellingShingle | Erhui Jin Yaqiong Pei Ting Liu Man Ren Qianqian Hu Youfang Gu Shenghe Li Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ Food and Agricultural Immunology estrogen receptor boron splenic lymphocytes t cell subset cytokine cell proliferation and apoptosis |
| title | Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ |
| title_full | Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ |
| title_fullStr | Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ |
| title_full_unstemmed | Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ |
| title_short | Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ |
| title_sort | effects of boron on the proliferation apoptosis and immune function of splenic lymphocytes through erα and erβ |
| topic | estrogen receptor boron splenic lymphocytes t cell subset cytokine cell proliferation and apoptosis |
| url | http://dx.doi.org/10.1080/09540105.2019.1626809 |
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