Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening
Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer and accounts for about 95% of all head and neck cancers with high mortality, usually at a late stage. Dysbiosis in the oral microbiome can lead to chronic inflammatory responses and may predispose to the development and progr...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023089818 |
| _version_ | 1827615943359987712 |
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| author | Bolaji Fatai Oyeyemi Urvinder S. Kaur Amit Paramraj Chintamani Ravi Tandon Abhinav Kumar Neel Sarovar Bhavesh |
| author_facet | Bolaji Fatai Oyeyemi Urvinder S. Kaur Amit Paramraj Chintamani Ravi Tandon Abhinav Kumar Neel Sarovar Bhavesh |
| author_sort | Bolaji Fatai Oyeyemi |
| collection | DOAJ |
| description | Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer and accounts for about 95% of all head and neck cancers with high mortality, usually at a late stage. Dysbiosis in the oral microbiome can lead to chronic inflammatory responses and may predispose to the development and progression of OSCC. Tobacco abuse plays an essential role in oral microbiome dysregulation and OSCC pathogenesis. We used 16S rRNA gene amplicon next-generation sequencing to examine microbial signatures unique to saliva from OSCC patients, tobacco abusers (TA) and controls (n = 10 for each group) to elucidate oral microbiome changes associated with tobacco abuse and OSCC. Overall, the oral microbiome compositions of class Betaproteobacteria and Epsilonproteobacteria, order Neisseriales, Burkholderiales and Campylobacterales, family Burkholderiaceae and Campylobacteraceae and genera Campylobacter and Leptotrichia revealed significant differences among OSCC patients, TA and control. Our preliminary pilot study not only serves as a basis for future studies with large sample size but also gives an indication of microbiome-based potential non-invasive biomarkers for early screening and monitoring of oral carcinogenesis transition due to tobacco abuse. |
| first_indexed | 2024-03-09T09:17:47Z |
| format | Article |
| id | doaj.art-62404b3c5cf5490fbf4a76195ee05550 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:17:47Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-62404b3c5cf5490fbf4a76195ee055502023-12-02T07:04:11ZengElsevierHeliyon2405-84402023-11-01911e21773Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screeningBolaji Fatai Oyeyemi0Urvinder S. Kaur1Amit Paramraj2 Chintamani3Ravi Tandon4Abhinav Kumar5Neel Sarovar Bhavesh6Transcription Regulation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Science Technology, The Federal Polytechnic, P.M.B. 5351, Ado-Ekiti, NigeriaLaboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, IndiaDepartment of Surgery, Vardhman Mahavir Medical College (VMMC)-Safdarjung Hospital, New Delhi, 110058, IndiaDepartment of Surgery, Vardhman Mahavir Medical College (VMMC)-Safdarjung Hospital, New Delhi, 110058, IndiaLaboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India; Corresponding author. Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, IndiaDepartment of Surgery, Vardhman Mahavir Medical College (VMMC)-Safdarjung Hospital, New Delhi, 110058, India; Division of Trauma Surgery and Critical Care, Department of Surgical Disciplines, Jai Prakash Narayan Apex Trauma Center, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India; Corresponding author. Division of Trauma Surgery and Critical Care, Department of Surgical Disciplines, Jai Prakash Narayan Apex Trauma Center, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, IndiaTranscription Regulation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India; Corresponding author. Transcription Regulation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, IndiaOral squamous cell carcinoma (OSCC) is the most common type of oral cancer and accounts for about 95% of all head and neck cancers with high mortality, usually at a late stage. Dysbiosis in the oral microbiome can lead to chronic inflammatory responses and may predispose to the development and progression of OSCC. Tobacco abuse plays an essential role in oral microbiome dysregulation and OSCC pathogenesis. We used 16S rRNA gene amplicon next-generation sequencing to examine microbial signatures unique to saliva from OSCC patients, tobacco abusers (TA) and controls (n = 10 for each group) to elucidate oral microbiome changes associated with tobacco abuse and OSCC. Overall, the oral microbiome compositions of class Betaproteobacteria and Epsilonproteobacteria, order Neisseriales, Burkholderiales and Campylobacterales, family Burkholderiaceae and Campylobacteraceae and genera Campylobacter and Leptotrichia revealed significant differences among OSCC patients, TA and control. Our preliminary pilot study not only serves as a basis for future studies with large sample size but also gives an indication of microbiome-based potential non-invasive biomarkers for early screening and monitoring of oral carcinogenesis transition due to tobacco abuse.http://www.sciencedirect.com/science/article/pii/S2405844023089818MicrobiomeNon-invasive biomarkerOral squamous cell carcinomaSalivaTobacco |
| spellingShingle | Bolaji Fatai Oyeyemi Urvinder S. Kaur Amit Paramraj Chintamani Ravi Tandon Abhinav Kumar Neel Sarovar Bhavesh Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening Heliyon Microbiome Non-invasive biomarker Oral squamous cell carcinoma Saliva Tobacco |
| title | Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening |
| title_full | Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening |
| title_fullStr | Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening |
| title_full_unstemmed | Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening |
| title_short | Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening |
| title_sort | microbiome analysis of saliva from oral squamous cell carcinoma oscc patients and tobacco abusers with potential biomarkers for oral cancer screening |
| topic | Microbiome Non-invasive biomarker Oral squamous cell carcinoma Saliva Tobacco |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023089818 |
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