Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients
Abstract Aim This study aimed to explore a novel subtype classification method based on the stemness characteristics of patients with non-small cell lung cancer (NSCLC). Methods Based on the Cancer Genome Atlas database to calculate the stemness index (mRNAsi) of NSCLC patients, an unsupervised cons...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-023-03406-4 |
| _version_ | 1797577742372306944 |
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| author | Mingshan Liu Ruihao Zhou Wei Zou Zhuofan Yang Quanjin Li Zhiguo Chen Lei jiang Jingtao Zhang |
| author_facet | Mingshan Liu Ruihao Zhou Wei Zou Zhuofan Yang Quanjin Li Zhiguo Chen Lei jiang Jingtao Zhang |
| author_sort | Mingshan Liu |
| collection | DOAJ |
| description | Abstract Aim This study aimed to explore a novel subtype classification method based on the stemness characteristics of patients with non-small cell lung cancer (NSCLC). Methods Based on the Cancer Genome Atlas database to calculate the stemness index (mRNAsi) of NSCLC patients, an unsupervised consensus clustering method was used to classify patients into two subtypes and analyze the survival differences, somatic mutational load, copy number variation, and immune characteristics differences between them. Subsequently, four machine learning methods were used to construct and validate a stemness subtype classification model, and cell function experiments were performed to verify the effect of the signature gene ARTN on NSCLC. Results Patients with Stemness Subtype I had better PFS and a higher somatic mutational burden and copy number alteration than patients with Stemness Subtype II. In addition, the two stemness subtypes have different patterns of tumor immune microenvironment. The immune score and stromal score and overall score of Stemness Subtype II were higher than those of Stemness Subtype I, suggesting a relatively small benefit to immune checkpoints. Four machine learning methods constructed and validated classification model for stemness subtypes and obtained multiple logistic regression equations for 22 characteristic genes. The results of cell function experiments showed that ARTN can promote the proliferation, invasion, and migration of NSCLC and is closely related to cancer stem cell properties. Conclusion This new classification method based on stemness characteristics can effectively distinguish patients' characteristics and thus provide possible directions for the selection and optimization of clinical treatment plans. |
| first_indexed | 2024-03-10T22:12:19Z |
| format | Article |
| id | doaj.art-62460e2b94524c93a8afb0d67dc43438 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-03-10T22:12:19Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-62460e2b94524c93a8afb0d67dc434382023-11-19T12:34:04ZengBMCStem Cell Research & Therapy1757-65122023-09-0114111510.1186/s13287-023-03406-4Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patientsMingshan Liu0Ruihao Zhou1Wei Zou2Zhuofan Yang3Quanjin Li4Zhiguo Chen5Lei jiang6Jingtao Zhang7Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityDepartment of Anesthesiology, West China Hospital, Sichuan UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityAbstract Aim This study aimed to explore a novel subtype classification method based on the stemness characteristics of patients with non-small cell lung cancer (NSCLC). Methods Based on the Cancer Genome Atlas database to calculate the stemness index (mRNAsi) of NSCLC patients, an unsupervised consensus clustering method was used to classify patients into two subtypes and analyze the survival differences, somatic mutational load, copy number variation, and immune characteristics differences between them. Subsequently, four machine learning methods were used to construct and validate a stemness subtype classification model, and cell function experiments were performed to verify the effect of the signature gene ARTN on NSCLC. Results Patients with Stemness Subtype I had better PFS and a higher somatic mutational burden and copy number alteration than patients with Stemness Subtype II. In addition, the two stemness subtypes have different patterns of tumor immune microenvironment. The immune score and stromal score and overall score of Stemness Subtype II were higher than those of Stemness Subtype I, suggesting a relatively small benefit to immune checkpoints. Four machine learning methods constructed and validated classification model for stemness subtypes and obtained multiple logistic regression equations for 22 characteristic genes. The results of cell function experiments showed that ARTN can promote the proliferation, invasion, and migration of NSCLC and is closely related to cancer stem cell properties. Conclusion This new classification method based on stemness characteristics can effectively distinguish patients' characteristics and thus provide possible directions for the selection and optimization of clinical treatment plans.https://doi.org/10.1186/s13287-023-03406-4Non-small cell lung cancerCancer stem cellStemness indexConsensus clusteringImmunotherapyMachine learning |
| spellingShingle | Mingshan Liu Ruihao Zhou Wei Zou Zhuofan Yang Quanjin Li Zhiguo Chen Lei jiang Jingtao Zhang Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients Stem Cell Research & Therapy Non-small cell lung cancer Cancer stem cell Stemness index Consensus clustering Immunotherapy Machine learning |
| title | Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients |
| title_full | Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients |
| title_fullStr | Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients |
| title_full_unstemmed | Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients |
| title_short | Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients |
| title_sort | machine learning identified stemness features and constructed stemness related subtype with prognosis chemotherapy and immunotherapy responses for non small cell lung cancer patients |
| topic | Non-small cell lung cancer Cancer stem cell Stemness index Consensus clustering Immunotherapy Machine learning |
| url | https://doi.org/10.1186/s13287-023-03406-4 |
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