Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
Bacterial community plays a key role in environmental and ecological processes of river ecosystems. Given the special climatic and geographical conditions, studying the compositional characteristics of microorganisms in highland rivers and the relationship between such microorganisms and water physi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2260 |
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| author | Qianqian Zhang Zhenbing Wu Juan Zhao Guojie Wang Jingwen Hao Shuyi Wang Yaoyao Lin Hongtao Guan Jinyong Zhang Shenglong Jian Aihua Li |
| author_facet | Qianqian Zhang Zhenbing Wu Juan Zhao Guojie Wang Jingwen Hao Shuyi Wang Yaoyao Lin Hongtao Guan Jinyong Zhang Shenglong Jian Aihua Li |
| author_sort | Qianqian Zhang |
| collection | DOAJ |
| description | Bacterial community plays a key role in environmental and ecological processes of river ecosystems. Given the special climatic and geographical conditions, studying the compositional characteristics of microorganisms in highland rivers and the relationship between such microorganisms and water physicochemical factors is important for an in-depth understanding of microbial ecological mechanisms. In the present study, high-throughput sequencing was used to investigate and study the bacterioplankton community of the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau. The results showed that the Huangshui River had significantly lower alpha diversity than the plain rivers. Despite the similarity in their environmental conditions, the main taxonomic compositions of the bacterial communities were distinct between the Huangshui River and polar regions (the Arctic and Antarctica). Proteobacteria accounted for the largest proportion (30.79–99.98%) of all the sequences, followed by Firmicutes (0–49.38%). <i>Acidiphilium</i> was the most numerous genera, which accounted for 0.03–86.16% of the assigned 16S reads, followed by <i>Acidocella</i> (0–95.9%), both belonging to Alphaproteobacteria. The diverse taxa of potential pathogens, such as Acinetobacter, Pseudomonas, and Aeromonas, were also identified. A principal coordinates analysis, coupled with a canonical correspondence analysis, showed spatial variations in the bacterial community composition. The water physical properties (e.g., Cr<sup>6+</sup>, total phosphorus, and COD<sub>Mn</sub>); altitude; and land use (e.g., urban land cover and aquaculture) determined the distribution of the bacterioplankton composition. PICRUSt2 revealed that the overall functional profiles of the bacterial communities in different samples were similar, and our results suggested the potential health risks of water sources in this area. This work provided valuable insight into the composition of the plankton bacterial community and its relationship with the environmental factors in the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau and a theoretical foundation for ecological health management. |
| first_indexed | 2024-03-10T05:15:46Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:46Z |
| publishDate | 2021-10-01 |
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| series | Microorganisms |
| spelling | doaj.art-624c83acd4c34308b2c2b6ef247fc5e02023-11-23T00:28:45ZengMDPI AGMicroorganisms2076-26072021-10-01911226010.3390/microorganisms9112260Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, ChinaQianqian Zhang0Zhenbing Wu1Juan Zhao2Guojie Wang3Jingwen Hao4Shuyi Wang5Yaoyao Lin6Hongtao Guan7Jinyong Zhang8Shenglong Jian9Aihua Li10State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaQinghai Provincial Fishery Environmental Monitoring Center, Xining 810012, ChinaQinghai Provincial Fishery Environmental Monitoring Center, Xining 810012, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaQinghai Provincial Fishery Environmental Monitoring Center, Xining 810012, ChinaThe Laboratory of Aquatic Parasitology, School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266237, ChinaQinghai Provincial Fishery Environmental Monitoring Center, Xining 810012, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaBacterial community plays a key role in environmental and ecological processes of river ecosystems. Given the special climatic and geographical conditions, studying the compositional characteristics of microorganisms in highland rivers and the relationship between such microorganisms and water physicochemical factors is important for an in-depth understanding of microbial ecological mechanisms. In the present study, high-throughput sequencing was used to investigate and study the bacterioplankton community of the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau. The results showed that the Huangshui River had significantly lower alpha diversity than the plain rivers. Despite the similarity in their environmental conditions, the main taxonomic compositions of the bacterial communities were distinct between the Huangshui River and polar regions (the Arctic and Antarctica). Proteobacteria accounted for the largest proportion (30.79–99.98%) of all the sequences, followed by Firmicutes (0–49.38%). <i>Acidiphilium</i> was the most numerous genera, which accounted for 0.03–86.16% of the assigned 16S reads, followed by <i>Acidocella</i> (0–95.9%), both belonging to Alphaproteobacteria. The diverse taxa of potential pathogens, such as Acinetobacter, Pseudomonas, and Aeromonas, were also identified. A principal coordinates analysis, coupled with a canonical correspondence analysis, showed spatial variations in the bacterial community composition. The water physical properties (e.g., Cr<sup>6+</sup>, total phosphorus, and COD<sub>Mn</sub>); altitude; and land use (e.g., urban land cover and aquaculture) determined the distribution of the bacterioplankton composition. PICRUSt2 revealed that the overall functional profiles of the bacterial communities in different samples were similar, and our results suggested the potential health risks of water sources in this area. This work provided valuable insight into the composition of the plankton bacterial community and its relationship with the environmental factors in the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau and a theoretical foundation for ecological health management.https://www.mdpi.com/2076-2607/9/11/2260Huangshui Riverhigh-throughput sequencingbacterioplankton community structureenvironment factorsPICRUSt2 analysis |
| spellingShingle | Qianqian Zhang Zhenbing Wu Juan Zhao Guojie Wang Jingwen Hao Shuyi Wang Yaoyao Lin Hongtao Guan Jinyong Zhang Shenglong Jian Aihua Li Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China Microorganisms Huangshui River high-throughput sequencing bacterioplankton community structure environment factors PICRUSt2 analysis |
| title | Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China |
| title_full | Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China |
| title_fullStr | Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China |
| title_full_unstemmed | Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China |
| title_short | Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China |
| title_sort | composition and functional characteristics and influencing factors of bacterioplankton community in the huangshui river china |
| topic | Huangshui River high-throughput sequencing bacterioplankton community structure environment factors PICRUSt2 analysis |
| url | https://www.mdpi.com/2076-2607/9/11/2260 |
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