Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes
Bloodstream infection with <i>Candida</i> fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to <i>Candida albicans</i>, the rising incidence of infections caused by other...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/3/255 |
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| author | Monsur Islam Devin Keck Jordon Gilmore Rodrigo Martinez-Duarte |
| author_facet | Monsur Islam Devin Keck Jordon Gilmore Rodrigo Martinez-Duarte |
| author_sort | Monsur Islam |
| collection | DOAJ |
| description | Bloodstream infection with <i>Candida</i> fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to <i>Candida albicans</i>, the rising incidence of infections caused by other <i>Candida </i>strains that may not respond to traditional anti-fungal treatments merits the development of a method for species-specific isolation of <i>Candida</i>. To this end, here we present the characterization of the dielectrophoresis (DEP) response of <i>Candida albicans</i>, <i>Candida tropicalis </i>and <i>Candida parapsilosis.</i> We complement such characterization with a study of the <i>Candida</i> cells morphology. The <i>Candida </i>strains exhibited subtle differences in their morphology and dimensions. All the <i>Candida </i>strains exhibited positive DEP in the range 10−500 kHz, although the strength of the DEP response was different for each <i>Candida </i>strain at different frequencies. Only <i>Candida tropicalis</i> showed positive DEP at 750 kHz. The current results show potential for manipulation and enrichment of a specific <i>Candida </i>strain at specific DEP conditions towards aiding in the rapid identification of <i>Candida </i>strains to enable the effective and timely treatment of <i>Candida </i>infections. |
| first_indexed | 2024-12-13T03:17:58Z |
| format | Article |
| id | doaj.art-624c991c6b954bc08a013b321d4d6836 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-13T03:17:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-624c991c6b954bc08a013b321d4d68362022-12-22T00:01:25ZengMDPI AGMicromachines2072-666X2020-02-0111325510.3390/mi11030255mi11030255Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon MicroelectrodesMonsur Islam0Devin Keck1Jordon Gilmore2Rodrigo Martinez-Duarte3Multiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USABloodstream infection with <i>Candida</i> fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to <i>Candida albicans</i>, the rising incidence of infections caused by other <i>Candida </i>strains that may not respond to traditional anti-fungal treatments merits the development of a method for species-specific isolation of <i>Candida</i>. To this end, here we present the characterization of the dielectrophoresis (DEP) response of <i>Candida albicans</i>, <i>Candida tropicalis </i>and <i>Candida parapsilosis.</i> We complement such characterization with a study of the <i>Candida</i> cells morphology. The <i>Candida </i>strains exhibited subtle differences in their morphology and dimensions. All the <i>Candida </i>strains exhibited positive DEP in the range 10−500 kHz, although the strength of the DEP response was different for each <i>Candida </i>strain at different frequencies. Only <i>Candida tropicalis</i> showed positive DEP at 750 kHz. The current results show potential for manipulation and enrichment of a specific <i>Candida </i>strain at specific DEP conditions towards aiding in the rapid identification of <i>Candida </i>strains to enable the effective and timely treatment of <i>Candida </i>infections.https://www.mdpi.com/2072-666X/11/3/255characterizationdielectrophoresiscarbon electrodesthree-dimensional (3d)diagnostics<i>candidiasis</i> |
| spellingShingle | Monsur Islam Devin Keck Jordon Gilmore Rodrigo Martinez-Duarte Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes Micromachines characterization dielectrophoresis carbon electrodes three-dimensional (3d) diagnostics <i>candidiasis</i> |
| title | Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes |
| title_full | Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes |
| title_fullStr | Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes |
| title_full_unstemmed | Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes |
| title_short | Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes |
| title_sort | characterization of the dielectrophoretic response of different candida strains using 3d carbon microelectrodes |
| topic | characterization dielectrophoresis carbon electrodes three-dimensional (3d) diagnostics <i>candidiasis</i> |
| url | https://www.mdpi.com/2072-666X/11/3/255 |
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