Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae)
Abstract Microplitis manilae Ashmead (Hymenoptera: Braconidae) is an important parasitoid of agricultural pests in lepidopteran species. So far, two extant genome assembles from the genus Microplitis are fragmented. Here, we offered a high-quality genome assembly of M. manilae at the chromosome leve...
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Nature Portfolio
2023-05-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-023-02190-3 |
| _version_ | 1827948267246190592 |
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| author | Xiaohan Shu Ruizhong Yuan Boying Zheng Zhizhi Wang Xiqian Ye Pu Tang Xuexin Chen |
| author_facet | Xiaohan Shu Ruizhong Yuan Boying Zheng Zhizhi Wang Xiqian Ye Pu Tang Xuexin Chen |
| author_sort | Xiaohan Shu |
| collection | DOAJ |
| description | Abstract Microplitis manilae Ashmead (Hymenoptera: Braconidae) is an important parasitoid of agricultural pests in lepidopteran species. So far, two extant genome assembles from the genus Microplitis are fragmented. Here, we offered a high-quality genome assembly of M. manilae at the chromosome level with high accuracy and contiguity, assembled by ONT long-read, MGI-SEQ short-read, and Hi-C sequencing methods. The final assembled genome size was 282.85 Mb, with 268.17 Mb assigned to 11 pseudochromosomes. The scaffold N50 length was 25.23 Mb, and the complete BUSCO score was 98.61%. The genome contained 152.37 Mb of repetitive elements, representing 53.87% of the total genome size. We predicted 15,689 protein-coding genes, of which 13,580 genes were annotated functionally. Gene family evolution investigations of M. manilae revealed 615 expanded and 635 contracted gene families. The high-quality genome of M. manilae reported in this paper will be a useful genomic resource for research on parasitoid wasps in the future. |
| first_indexed | 2024-04-09T12:52:40Z |
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| id | doaj.art-624ee4dc76ae4aea878c29b51a3c0ad9 |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-04-09T12:52:40Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-624ee4dc76ae4aea878c29b51a3c0ad92023-05-14T11:07:54ZengNature PortfolioScientific Data2052-44632023-05-011011910.1038/s41597-023-02190-3Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae)Xiaohan Shu0Ruizhong Yuan1Boying Zheng2Zhizhi Wang3Xiqian Ye4Pu Tang5Xuexin Chen6Hainan Institute, Zhejiang UniversityGuangdong Laboratory for Lingnan Modern AgricultureState Key Lab of Rice Biology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, and Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects, Zhejiang UniversityGuangdong Laboratory for Lingnan Modern AgricultureGuangdong Laboratory for Lingnan Modern AgricultureHainan Institute, Zhejiang UniversityHainan Institute, Zhejiang UniversityAbstract Microplitis manilae Ashmead (Hymenoptera: Braconidae) is an important parasitoid of agricultural pests in lepidopteran species. So far, two extant genome assembles from the genus Microplitis are fragmented. Here, we offered a high-quality genome assembly of M. manilae at the chromosome level with high accuracy and contiguity, assembled by ONT long-read, MGI-SEQ short-read, and Hi-C sequencing methods. The final assembled genome size was 282.85 Mb, with 268.17 Mb assigned to 11 pseudochromosomes. The scaffold N50 length was 25.23 Mb, and the complete BUSCO score was 98.61%. The genome contained 152.37 Mb of repetitive elements, representing 53.87% of the total genome size. We predicted 15,689 protein-coding genes, of which 13,580 genes were annotated functionally. Gene family evolution investigations of M. manilae revealed 615 expanded and 635 contracted gene families. The high-quality genome of M. manilae reported in this paper will be a useful genomic resource for research on parasitoid wasps in the future.https://doi.org/10.1038/s41597-023-02190-3 |
| spellingShingle | Xiaohan Shu Ruizhong Yuan Boying Zheng Zhizhi Wang Xiqian Ye Pu Tang Xuexin Chen Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae) Scientific Data |
| title | Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae) |
| title_full | Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae) |
| title_fullStr | Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae) |
| title_full_unstemmed | Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae) |
| title_short | Chromosome-level genome assembly of Microplitis manilae Ashmead, 1904 (Hymenoptera: Braconidae) |
| title_sort | chromosome level genome assembly of microplitis manilae ashmead 1904 hymenoptera braconidae |
| url | https://doi.org/10.1038/s41597-023-02190-3 |
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