Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis
The fabrication of 3D microstructures is under continuous development for engineering bone substitutes. Collagen/chitosan (Col/CT) blends emerge as biomaterials that meet the mechanical and biological requirements associated with bone tissue. In this work, we optimize the osteogenic effect of 3D mic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/17/6426 |
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| author | Irina Alexandra Păun Cosmin Cătălin Mustăciosu Roxana Cristina Popescu Bogdan Ştefăniţă Călin Mona Mihăilescu |
| author_facet | Irina Alexandra Păun Cosmin Cătălin Mustăciosu Roxana Cristina Popescu Bogdan Ştefăniţă Călin Mona Mihăilescu |
| author_sort | Irina Alexandra Păun |
| collection | DOAJ |
| description | The fabrication of 3D microstructures is under continuous development for engineering bone substitutes. Collagen/chitosan (Col/CT) blends emerge as biomaterials that meet the mechanical and biological requirements associated with bone tissue. In this work, we optimize the osteogenic effect of 3D microstructures by their functionalization with Col/CT blends with different blending ratios. The structures were fabricated by laser direct writing via two-photons polymerization of IP-L780 photopolymer. They comprised of hexagonal and ellipsoidal units 80 µm in length, 40 µm in width and 14 µm height, separated by 20 µm pillars. Structures’ functionalization was achieved via dip coating in Col/CT blends with specific blending ratios. The osteogenic role of Col/CT functionalization of the 3D structures was confirmed by biological assays concerning the expression of alkaline phosphatase (ALP) and osteocalcin secretion as osteogenic markers and Alizarin Red (AR) as dye for mineral deposits in osteoblast-like cells seeded on the structures. The structures having ellipsoidal units showed the best results, but the trends were similar for both ellipsoidal and hexagonal units. The strongest osteogenic effect was obtained for Col/CT blending ratio of 20/80, as demonstrated by the highest ALP activity, osteocalcin secretion and AR staining intensity in the seeded cells compared to all the other samples. |
| first_indexed | 2024-03-10T16:35:49Z |
| format | Article |
| id | doaj.art-6250c617b30545d4a0a41a47bd14c0f6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:35:49Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6250c617b30545d4a0a41a47bd14c0f62023-11-20T12:28:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012117642610.3390/ijms21176426Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced OsteogenesisIrina Alexandra Păun0Cosmin Cătălin Mustăciosu1Roxana Cristina Popescu2Bogdan Ştefăniţă Călin3Mona Mihăilescu4Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele, RomaniaDepartment of Life and Environmental Physics, Horia Hulubei National Institute for Physics and Nuclear Engineering, Reactorului 30, RO-077125 Magurele, RomaniaDepartment of Life and Environmental Physics, Horia Hulubei National Institute for Physics and Nuclear Engineering, Reactorului 30, RO-077125 Magurele, RomaniaCenter for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele, RomaniaFaculty of Applied Sciences, University Politehnica of Bucharest, Splaiul Independentei 313, RO-060042 Bucharest, RomaniaThe fabrication of 3D microstructures is under continuous development for engineering bone substitutes. Collagen/chitosan (Col/CT) blends emerge as biomaterials that meet the mechanical and biological requirements associated with bone tissue. In this work, we optimize the osteogenic effect of 3D microstructures by their functionalization with Col/CT blends with different blending ratios. The structures were fabricated by laser direct writing via two-photons polymerization of IP-L780 photopolymer. They comprised of hexagonal and ellipsoidal units 80 µm in length, 40 µm in width and 14 µm height, separated by 20 µm pillars. Structures’ functionalization was achieved via dip coating in Col/CT blends with specific blending ratios. The osteogenic role of Col/CT functionalization of the 3D structures was confirmed by biological assays concerning the expression of alkaline phosphatase (ALP) and osteocalcin secretion as osteogenic markers and Alizarin Red (AR) as dye for mineral deposits in osteoblast-like cells seeded on the structures. The structures having ellipsoidal units showed the best results, but the trends were similar for both ellipsoidal and hexagonal units. The strongest osteogenic effect was obtained for Col/CT blending ratio of 20/80, as demonstrated by the highest ALP activity, osteocalcin secretion and AR staining intensity in the seeded cells compared to all the other samples.https://www.mdpi.com/1422-0067/21/17/6426laser direct writing3D structurecollagen/chitosan functionalizationosteogenesis |
| spellingShingle | Irina Alexandra Păun Cosmin Cătălin Mustăciosu Roxana Cristina Popescu Bogdan Ştefăniţă Călin Mona Mihăilescu Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis International Journal of Molecular Sciences laser direct writing 3D structure collagen/chitosan functionalization osteogenesis |
| title | Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis |
| title_full | Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis |
| title_fullStr | Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis |
| title_full_unstemmed | Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis |
| title_short | Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis |
| title_sort | collagen chitosan functionalization of complex 3d structures fabricated by laser direct writing via two photon polymerization for enhanced osteogenesis |
| topic | laser direct writing 3D structure collagen/chitosan functionalization osteogenesis |
| url | https://www.mdpi.com/1422-0067/21/17/6426 |
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