From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet
A widely used approach to preserving genetic diversity in birds involves the cryopreservation of semen. In this process, cells are subjected to physical and chemical stresses, but not all cell species respond equally. Many studies have been published on the freezing–thawing of sperm cells from a wid...
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MDPI AG
2021-09-01
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| author | Annelisse Castillo Carla Lenzi Andrea Pirone Alessandro Baglini Claudia Russo Dominga Soglia Achille Schiavone Margherita Marzoni Fecia di Cossato |
| author_facet | Annelisse Castillo Carla Lenzi Andrea Pirone Alessandro Baglini Claudia Russo Dominga Soglia Achille Schiavone Margherita Marzoni Fecia di Cossato |
| author_sort | Annelisse Castillo |
| collection | DOAJ |
| description | A widely used approach to preserving genetic diversity in birds involves the cryopreservation of semen. In this process, cells are subjected to physical and chemical stresses, but not all cell species respond equally. Many studies have been published on the freezing–thawing of sperm cells from a wide variety of domestic and wild species, on issues ranging from the sperm quality to different protocols, fertilisation success rates, etc. Nevertheless, very little information is available on the common pheasant. To fill this gap, the aim of this study was to describe the pheasant semen collection method, evaluate some qualitative parameters of sperm from males fed an antioxidant-enriched diet, and to test the in vivo fertilising capacity of the cryo-preserved semen. The freezing protocol employed involved pellets thawed by the hotplate method. Dimethylacetamide was used as a cryoprotectant at a final concentration of 6%. A total of six AIs were performed at 3-4-day intervals on a total of 40 females with doses of 35 × 10<sup>6</sup> of normal live thawed sperm. Males receiving the enriched diet produce more abundant and concentrated ejaculates. Freeze–thawed sperm lost 85% of their initial mobility, and diet influenced neither sperm mobility nor viability. The enriched diet did improve the number of normal freeze–thawed cells and was associated with a lower sperm fracture incidence. Regardless of the dietary group, frozen–thawed sperm resulted in a fertility rate of 30%, with 8-9 chicks hatching for every 100 eggs incubated. |
| first_indexed | 2024-03-10T07:58:02Z |
| format | Article |
| id | doaj.art-62522eb247b74993927479a07537f91d |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T07:58:02Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-62522eb247b74993927479a07537f91d2023-11-22T11:42:58ZengMDPI AGAnimals2076-26152021-09-01119262410.3390/ani11092624From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched DietAnnelisse Castillo0Carla Lenzi1Andrea Pirone2Alessandro Baglini3Claudia Russo4Dominga Soglia5Achille Schiavone6Margherita Marzoni Fecia di Cossato7Dipartimento di Scienze Veterinarie, Università Degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, TO, ItalyDipartimento di Scienze Veterinarie, Università di Pisa, Viale Delle Piagge 2, 56124 Pisa, PI, ItalyDipartimento di Scienze Veterinarie, Università di Pisa, Viale Delle Piagge 2, 56124 Pisa, PI, ItalyDipartimento di Scienze Veterinarie, Università di Pisa, Viale Delle Piagge 2, 56124 Pisa, PI, ItalyDipartimento di Scienze Veterinarie, Università di Pisa, Viale Delle Piagge 2, 56124 Pisa, PI, ItalyDipartimento di Scienze Veterinarie, Università Degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, TO, ItalyDipartimento di Scienze Veterinarie, Università Degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, TO, ItalyDipartimento di Scienze Veterinarie, Università di Pisa, Viale Delle Piagge 2, 56124 Pisa, PI, ItalyA widely used approach to preserving genetic diversity in birds involves the cryopreservation of semen. In this process, cells are subjected to physical and chemical stresses, but not all cell species respond equally. Many studies have been published on the freezing–thawing of sperm cells from a wide variety of domestic and wild species, on issues ranging from the sperm quality to different protocols, fertilisation success rates, etc. Nevertheless, very little information is available on the common pheasant. To fill this gap, the aim of this study was to describe the pheasant semen collection method, evaluate some qualitative parameters of sperm from males fed an antioxidant-enriched diet, and to test the in vivo fertilising capacity of the cryo-preserved semen. The freezing protocol employed involved pellets thawed by the hotplate method. Dimethylacetamide was used as a cryoprotectant at a final concentration of 6%. A total of six AIs were performed at 3-4-day intervals on a total of 40 females with doses of 35 × 10<sup>6</sup> of normal live thawed sperm. Males receiving the enriched diet produce more abundant and concentrated ejaculates. Freeze–thawed sperm lost 85% of their initial mobility, and diet influenced neither sperm mobility nor viability. The enriched diet did improve the number of normal freeze–thawed cells and was associated with a lower sperm fracture incidence. Regardless of the dietary group, frozen–thawed sperm resulted in a fertility rate of 30%, with 8-9 chicks hatching for every 100 eggs incubated.https://www.mdpi.com/2076-2615/11/9/2624pheasant semenfreezing processvitamin EseleniumAIhatchability |
| spellingShingle | Annelisse Castillo Carla Lenzi Andrea Pirone Alessandro Baglini Claudia Russo Dominga Soglia Achille Schiavone Margherita Marzoni Fecia di Cossato From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet Animals pheasant semen freezing process vitamin E selenium AI hatchability |
| title | From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet |
| title_full | From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet |
| title_fullStr | From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet |
| title_full_unstemmed | From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet |
| title_short | From the Semen Collection Method to the Hatchlings: The Use of Cryopreserved Sperm from Pheasants Fed an Antioxidant-Enriched Diet |
| title_sort | from the semen collection method to the hatchlings the use of cryopreserved sperm from pheasants fed an antioxidant enriched diet |
| topic | pheasant semen freezing process vitamin E selenium AI hatchability |
| url | https://www.mdpi.com/2076-2615/11/9/2624 |
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