Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry
Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the ai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/9/11452 |
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| author | Tung-Hu Tsai Lie-Chwen Lin Li-Wen Chang Yin-Meng Tsai Chia-Jung Lee Mei-Ling Hou Wan-Ling Chang-Liao |
| author_facet | Tung-Hu Tsai Lie-Chwen Lin Li-Wen Chang Yin-Meng Tsai Chia-Jung Lee Mei-Ling Hou Wan-Ling Chang-Liao |
| author_sort | Tung-Hu Tsai |
| collection | DOAJ |
| description | Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetate-methanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLC-MS/MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats. |
| first_indexed | 2024-04-13T09:35:38Z |
| format | Article |
| id | doaj.art-625a440d9122449798dbc648b6856524 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T09:35:38Z |
| publishDate | 2013-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-625a440d9122449798dbc648b68565242022-12-22T02:52:06ZengMDPI AGMolecules1420-30492013-09-01189114521146610.3390/molecules180911452Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass SpectrometryTung-Hu TsaiLie-Chwen LinLi-Wen ChangYin-Meng TsaiChia-Jung LeeMei-Ling HouWan-Ling Chang-LiaoDi-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetate-methanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLC-MS/MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.http://www.mdpi.com/1420-3049/18/9/11452di-(2-ethylhexyl) phthalate (DEHP)fecal excretionpharmacokineticsphthalatesplasticizer |
| spellingShingle | Tung-Hu Tsai Lie-Chwen Lin Li-Wen Chang Yin-Meng Tsai Chia-Jung Lee Mei-Ling Hou Wan-Ling Chang-Liao Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry Molecules di-(2-ethylhexyl) phthalate (DEHP) fecal excretion pharmacokinetics phthalates plasticizer |
| title | Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_full | Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_fullStr | Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_full_unstemmed | Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_short | Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_sort | determination and pharmacokinetics of di 2 ethylhexyl phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry |
| topic | di-(2-ethylhexyl) phthalate (DEHP) fecal excretion pharmacokinetics phthalates plasticizer |
| url | http://www.mdpi.com/1420-3049/18/9/11452 |
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