The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos
The cloning between different animals known as interspecific somatic cell nuclear transfer (iSCNT) was carried out for endangered species. The iSCNT has been characterized by a poor success rate due to several factors that influence the formation of the SCNT in various cytoplasms. The cell cycle of...
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-04-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2022-0153 |
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| author | Ammari Aiman A. ALghadi Muath G. ALhimaidi Ahmad R. Amran Ramzi A. |
| author_facet | Ammari Aiman A. ALghadi Muath G. ALhimaidi Ahmad R. Amran Ramzi A. |
| author_sort | Ammari Aiman A. |
| collection | DOAJ |
| description | The cloning between different animals known as interspecific somatic cell nuclear transfer (iSCNT) was carried out for endangered species. The iSCNT has been characterized by a poor success rate due to several factors that influence the formation of the SCNT in various cytoplasms. The cell cycle of the transferred somatic cell, the passage number of the cultured somatic cell, the mitochondria oocytes, and their capabilities are among these factors. This study investigates the role of the passage number of the Arabian Oryx somatic cell culture when transplanted to an enucleated domestic cow oocyte and embryo development in vitro. The fibroblast somatic cell of the Arabian Oryx was cultured for several passage lanes (3–13). The optimal passage cell number was found to be 10–13 Oryx cell lines that progressed to various cell stages up to the blastula stage. There was some variation between the different passage numbers of the oryx cell line. The 3–9 cell line did not show a good developmental stage. These could be attributed to several factors that control the iSCNT as stated by several investigators. More investigation is needed to clarify the role of factors that affect the success rate for the iSCNT. |
| first_indexed | 2024-04-12T12:09:02Z |
| format | Article |
| id | doaj.art-625acebf1d2e4d8982ae377e45d85ff5 |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-04-12T12:09:02Z |
| publishDate | 2022-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-625acebf1d2e4d8982ae377e45d85ff52022-12-22T03:33:37ZengDe GruyterOpen Chemistry2391-54202022-04-0120134234910.1515/chem-2022-0153The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryosAmmari Aiman A.0ALghadi Muath G.1ALhimaidi Ahmad R.2Amran Ramzi A.3Department of Zoology, King Saud University, College of Science, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi ArabiaDepartment of Zoology, King Saud University, College of Science, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi ArabiaDepartment of Zoology, King Saud University, College of Science, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi ArabiaDepartment of Zoology, King Saud University, College of Science, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi ArabiaThe cloning between different animals known as interspecific somatic cell nuclear transfer (iSCNT) was carried out for endangered species. The iSCNT has been characterized by a poor success rate due to several factors that influence the formation of the SCNT in various cytoplasms. The cell cycle of the transferred somatic cell, the passage number of the cultured somatic cell, the mitochondria oocytes, and their capabilities are among these factors. This study investigates the role of the passage number of the Arabian Oryx somatic cell culture when transplanted to an enucleated domestic cow oocyte and embryo development in vitro. The fibroblast somatic cell of the Arabian Oryx was cultured for several passage lanes (3–13). The optimal passage cell number was found to be 10–13 Oryx cell lines that progressed to various cell stages up to the blastula stage. There was some variation between the different passage numbers of the oryx cell line. The 3–9 cell line did not show a good developmental stage. These could be attributed to several factors that control the iSCNT as stated by several investigators. More investigation is needed to clarify the role of factors that affect the success rate for the iSCNT.https://doi.org/10.1515/chem-2022-0153iscntenucleationpassageembryooryx |
| spellingShingle | Ammari Aiman A. ALghadi Muath G. ALhimaidi Ahmad R. Amran Ramzi A. The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos Open Chemistry iscnt enucleation passage embryo oryx |
| title | The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos |
| title_full | The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos |
| title_fullStr | The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos |
| title_full_unstemmed | The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos |
| title_short | The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos |
| title_sort | role of passage numbers of donor cells in the development of arabian oryx cow interspecific somatic cell nuclear transfer embryos |
| topic | iscnt enucleation passage embryo oryx |
| url | https://doi.org/10.1515/chem-2022-0153 |
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