Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects
Mass spectrometry is a modern physical-chemical analytical method that provides for qualitative and quantitative assessment of the substance composition. It is based on pre-ionization of the atoms and molecules included into it. One of the advanced methods of ionization, due to which mass-spectromet...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2014-09-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/169 |
| _version_ | 1797220869515247616 |
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| author | A. N. Spitsyn D. V. Utkin V. E. Kuklev S. A. Portenko V. G. Germanchuk N. A. Osina |
| author_facet | A. N. Spitsyn D. V. Utkin V. E. Kuklev S. A. Portenko V. G. Germanchuk N. A. Osina |
| author_sort | A. N. Spitsyn |
| collection | DOAJ |
| description | Mass spectrometry is a modern physical-chemical analytical method that provides for qualitative and quantitative assessment of the substance composition. It is based on pre-ionization of the atoms and molecules included into it. One of the advanced methods of ionization, due to which mass-spectrometry investigation of macromolecules has become a frequent practice, is matrix-assisted laser desorption/ionization (MALDI). The essence of it is the pulsed laser irradiation of the matter under study, mixed with the matrix. The review discusses current data on MALDI mass-spectrometry application for the performance of species-specific and genus-specific identification of microorganisms at the premises of diagnostic laboratories. Considered are the basic advantages of MALDI-TOF identification as compared to bacteriologic, immunologic, and molecular-genetic methods of assessment. Allocated is the mass-spectrometry position in the system of laboratory diagnostics of infectious diseases, including particularly dangerous ones, in the territory of the Russian Federation. |
| first_indexed | 2024-03-08T13:43:25Z |
| format | Article |
| id | doaj.art-625b0f21911c4034a67232ed99ef944e |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:24Z |
| publishDate | 2014-09-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-625b0f21911c4034a67232ed99ef944e2024-04-05T16:47:10ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2014-09-0103778210.21055/0370-1069-2014-3-77-82169Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and ProspectsA. N. Spitsyn0D. V. Utkin1V. E. Kuklev2S. A. Portenko3V. G. Germanchuk4N. A. Osina5Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Mass spectrometry is a modern physical-chemical analytical method that provides for qualitative and quantitative assessment of the substance composition. It is based on pre-ionization of the atoms and molecules included into it. One of the advanced methods of ionization, due to which mass-spectrometry investigation of macromolecules has become a frequent practice, is matrix-assisted laser desorption/ionization (MALDI). The essence of it is the pulsed laser irradiation of the matter under study, mixed with the matrix. The review discusses current data on MALDI mass-spectrometry application for the performance of species-specific and genus-specific identification of microorganisms at the premises of diagnostic laboratories. Considered are the basic advantages of MALDI-TOF identification as compared to bacteriologic, immunologic, and molecular-genetic methods of assessment. Allocated is the mass-spectrometry position in the system of laboratory diagnostics of infectious diseases, including particularly dangerous ones, in the territory of the Russian Federation.https://journal.microbe.ru/jour/article/view/169maldi масс-спектрометрияmaldi-tofидентификацияпатогенные биологические агентыmaldi mass-spectrometrymaldi-tofidentificationpathogenic biological agents |
| spellingShingle | A. N. Spitsyn D. V. Utkin V. E. Kuklev S. A. Portenko V. G. Germanchuk N. A. Osina Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects Проблемы особо опасных инфекций maldi масс-спектрометрия maldi-tof идентификация патогенные биологические агенты maldi mass-spectrometry maldi-tof identification pathogenic biological agents |
| title | Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects |
| title_full | Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects |
| title_fullStr | Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects |
| title_full_unstemmed | Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects |
| title_short | Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects |
| title_sort | application of maldi mass spectrometry for diagnostics of particularly dangerous infectious diseases current state of affairs and prospects |
| topic | maldi масс-спектрометрия maldi-tof идентификация патогенные биологические агенты maldi mass-spectrometry maldi-tof identification pathogenic biological agents |
| url | https://journal.microbe.ru/jour/article/view/169 |
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