Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species
<i>Euphorbia</i> is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen <i>Euphorbia</i> species revealed highest activity of <i>E. officinarum</i> L. against human colon adenocarcinoma (CACO2)...
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MDPI AG
2020-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/1/15 |
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| author | Seham S. El-Hawary Rabab Mohammed Ahmed F. Tawfike Nadia M. Lithy Sameh Fekry AbouZid Mohamed N. Amin Usama Ramadan Abdelmohsen Elham Amin |
| author_facet | Seham S. El-Hawary Rabab Mohammed Ahmed F. Tawfike Nadia M. Lithy Sameh Fekry AbouZid Mohamed N. Amin Usama Ramadan Abdelmohsen Elham Amin |
| author_sort | Seham S. El-Hawary |
| collection | DOAJ |
| description | <i>Euphorbia</i> is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen <i>Euphorbia</i> species revealed highest activity of <i>E. officinarum</i> L. against human colon adenocarcinoma (CACO2) cell line (IC<sub>50</sub> 7.2 µM) and of <i>E. lactea</i> Haw. against human hepatoma (HepG2) and human breast adenocarcinoma (MCF-7) cell lines (IC<sub>50</sub> 5.2 and 5.1 µM, respectively). Additionally, metabolic profiling of the fifteen tested species, using LC-HRMS, for dereplication purposes, led to the annotation of 44 natural compounds. Among the annotated compounds, diterpenoids represent the major class. Dereplication approach and multivariate data analysis are adopted in order to annotate the compounds responsible for the detected cytotoxic activity. Results of Principle component analysis (PCA) come in a great accordance with results of biological testing, which emphasized the cytotoxic properties of <i>E. lactea</i> Haw. A similarity correlation network showed that the two compounds with the molecular formula C<sub>16</sub>H<sub>18</sub>O<sub>8</sub> and C<sub>20</sub>H<sub>30</sub>O<sub>10</sub>, are responsible for cytotoxic activity against MCF-7 and HepG2 cell lines. Similarly, the compound with molecular formula C<sub>18</sub>H<sub>35</sub>NO correlates with cytotoxic activity against CACO2. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T13:41:37Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-625cd975589e4f3594705174602da2822023-11-21T02:56:53ZengMDPI AGMetabolites2218-19892020-12-011111510.3390/metabo11010015Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> SpeciesSeham S. El-Hawary0Rabab Mohammed1Ahmed F. Tawfike2Nadia M. Lithy3Sameh Fekry AbouZid4Mohamed N. Amin5Usama Ramadan Abdelmohsen6Elham Amin7Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Giza 12613, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo 11795, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62521, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt<i>Euphorbia</i> is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen <i>Euphorbia</i> species revealed highest activity of <i>E. officinarum</i> L. against human colon adenocarcinoma (CACO2) cell line (IC<sub>50</sub> 7.2 µM) and of <i>E. lactea</i> Haw. against human hepatoma (HepG2) and human breast adenocarcinoma (MCF-7) cell lines (IC<sub>50</sub> 5.2 and 5.1 µM, respectively). Additionally, metabolic profiling of the fifteen tested species, using LC-HRMS, for dereplication purposes, led to the annotation of 44 natural compounds. Among the annotated compounds, diterpenoids represent the major class. Dereplication approach and multivariate data analysis are adopted in order to annotate the compounds responsible for the detected cytotoxic activity. Results of Principle component analysis (PCA) come in a great accordance with results of biological testing, which emphasized the cytotoxic properties of <i>E. lactea</i> Haw. A similarity correlation network showed that the two compounds with the molecular formula C<sub>16</sub>H<sub>18</sub>O<sub>8</sub> and C<sub>20</sub>H<sub>30</sub>O<sub>10</sub>, are responsible for cytotoxic activity against MCF-7 and HepG2 cell lines. Similarly, the compound with molecular formula C<sub>18</sub>H<sub>35</sub>NO correlates with cytotoxic activity against CACO2.https://www.mdpi.com/2218-1989/11/1/15<i>Euphorbia</i>cytotoxic activitymetabolic profilingLC-HRMSPCA |
| spellingShingle | Seham S. El-Hawary Rabab Mohammed Ahmed F. Tawfike Nadia M. Lithy Sameh Fekry AbouZid Mohamed N. Amin Usama Ramadan Abdelmohsen Elham Amin Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species Metabolites <i>Euphorbia</i> cytotoxic activity metabolic profiling LC-HRMS PCA |
| title | Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species |
| title_full | Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species |
| title_fullStr | Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species |
| title_full_unstemmed | Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species |
| title_short | Cytotoxic Activity and Metabolic Profiling of Fifteen <i>Euphorbia</i> Species |
| title_sort | cytotoxic activity and metabolic profiling of fifteen i euphorbia i species |
| topic | <i>Euphorbia</i> cytotoxic activity metabolic profiling LC-HRMS PCA |
| url | https://www.mdpi.com/2218-1989/11/1/15 |
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