Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L.
Stress-inducible promoters are vital for the desirable expression of genes, especially transcription factors, which could otherwise compromise growth and development when constitutively overexpressed in plants. Here, we report on the characterization of the promoter region of a stress-responsive gen...
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MDPI AG
2022-12-01
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| author | Sonali Sengupta Necla Pehlivan Venkata Mangu Kanniah Rajasekaran Niranjan Baisakh |
| author_facet | Sonali Sengupta Necla Pehlivan Venkata Mangu Kanniah Rajasekaran Niranjan Baisakh |
| author_sort | Sonali Sengupta |
| collection | DOAJ |
| description | Stress-inducible promoters are vital for the desirable expression of genes, especially transcription factors, which could otherwise compromise growth and development when constitutively overexpressed in plants. Here, we report on the characterization of the promoter region of a stress-responsive gene <i>SaAsr1</i> from monocot halophyte cordgrass (<i>Spartina alterniflora</i>). Several <i>cis</i>-acting elements, such as ABRE (ABA-responsive element), DRE-CRT (dehydration responsive-element/C-Repeat), LTRE (low temperature-responsive element), ERE (ethylene-responsive element), LRE (light-responsive element), etc. contributed at varying degrees to salt-, drought- and ABA-enhanced expression of <i>gusA</i> reporter gene in <i>Arabidopsis thaliana</i> under the full-length promoter, pAsr1<sub>1875</sub> and its deletion derivatives with an assortment of <i>cis</i>-regulatory motifs. The smallest promoter, pAsr1<sub>491</sub>, with three <i>cis</i>-acting elements (a CCAAT box-heat responsive, an LRE, and a copper responsive element) conferred drought-enhanced expression of <i>gusA</i>; pAsr1<sub>755</sub> (with an ABRE and a DRE) presented the highest expression in ABA and drought; and pAsr1<sub>994</sub> with seven ABREs and two DREs conferred optimal induction of <i>gusA</i>, especially under drought and ABA. <i>Arabidopsis</i> transgenics expressing a known abiotic stress-responsive gene, <i>SaADF2</i> (actin depolymerization factor 2), under both pAsr1<sub>1875</sub> and p35S promoters outperformed the wild type (WT) with enhanced drought and salt tolerance contributed by higher relative water content and membrane stability with no significant difference between pAsr1<sub>1875</sub>:<i>SaADF2</i> or p35S:<i>SaADF2</i> lines. However, pAsr1<sub>1875</sub>:<i>SaADF2</i> lines produced healthy plants with robust shoot systems under salt stress and control compared to slightly stunted growth of the p35S:<i>SaADF2</i> plants. This reestablished the evidence that transgene expression under a stress-inducible promoter is a better strategy for the genetic manipulation of crops. |
| first_indexed | 2024-03-09T17:18:12Z |
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| last_indexed | 2024-03-09T17:18:12Z |
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| spelling | doaj.art-625de496d23e49068d2aceb2e95bab882023-11-24T13:24:14ZengMDPI AGBiology2079-77372022-12-011112182810.3390/biology11121828Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L.Sonali Sengupta0Necla Pehlivan1Venkata Mangu2Kanniah Rajasekaran3Niranjan Baisakh4School of Plant, Environmental and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USASchool of Plant, Environmental and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USASchool of Plant, Environmental and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USASouthern Regional Research Center, New Orleans, LA 77054, USASchool of Plant, Environmental and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USAStress-inducible promoters are vital for the desirable expression of genes, especially transcription factors, which could otherwise compromise growth and development when constitutively overexpressed in plants. Here, we report on the characterization of the promoter region of a stress-responsive gene <i>SaAsr1</i> from monocot halophyte cordgrass (<i>Spartina alterniflora</i>). Several <i>cis</i>-acting elements, such as ABRE (ABA-responsive element), DRE-CRT (dehydration responsive-element/C-Repeat), LTRE (low temperature-responsive element), ERE (ethylene-responsive element), LRE (light-responsive element), etc. contributed at varying degrees to salt-, drought- and ABA-enhanced expression of <i>gusA</i> reporter gene in <i>Arabidopsis thaliana</i> under the full-length promoter, pAsr1<sub>1875</sub> and its deletion derivatives with an assortment of <i>cis</i>-regulatory motifs. The smallest promoter, pAsr1<sub>491</sub>, with three <i>cis</i>-acting elements (a CCAAT box-heat responsive, an LRE, and a copper responsive element) conferred drought-enhanced expression of <i>gusA</i>; pAsr1<sub>755</sub> (with an ABRE and a DRE) presented the highest expression in ABA and drought; and pAsr1<sub>994</sub> with seven ABREs and two DREs conferred optimal induction of <i>gusA</i>, especially under drought and ABA. <i>Arabidopsis</i> transgenics expressing a known abiotic stress-responsive gene, <i>SaADF2</i> (actin depolymerization factor 2), under both pAsr1<sub>1875</sub> and p35S promoters outperformed the wild type (WT) with enhanced drought and salt tolerance contributed by higher relative water content and membrane stability with no significant difference between pAsr1<sub>1875</sub>:<i>SaADF2</i> or p35S:<i>SaADF2</i> lines. However, pAsr1<sub>1875</sub>:<i>SaADF2</i> lines produced healthy plants with robust shoot systems under salt stress and control compared to slightly stunted growth of the p35S:<i>SaADF2</i> plants. This reestablished the evidence that transgene expression under a stress-inducible promoter is a better strategy for the genetic manipulation of crops.https://www.mdpi.com/2079-7737/11/12/1828abiotic stressgenetic engineeringhalophytepromoterstress-inducible |
| spellingShingle | Sonali Sengupta Necla Pehlivan Venkata Mangu Kanniah Rajasekaran Niranjan Baisakh Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L. Biology abiotic stress genetic engineering halophyte promoter stress-inducible |
| title | Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L. |
| title_full | Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L. |
| title_fullStr | Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L. |
| title_full_unstemmed | Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L. |
| title_short | Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, <i>Spartina alterniflora</i> L. |
| title_sort | characterization of a stress enhanced promoter from the grass halophyte i spartina alterniflora i l |
| topic | abiotic stress genetic engineering halophyte promoter stress-inducible |
| url | https://www.mdpi.com/2079-7737/11/12/1828 |
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