Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum
Abstract Background Oral vaccination with Mycobacterium bovis Bacille of Calmette and Guerin (BCG) has provided protection against M. bovis to badgers both experimentally and in the field. There is also evidence suggesting that the persistence of live BCG within the host is important for maintaining...
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| Language: | English |
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BMC
2019-12-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-019-2166-4 |
| _version_ | 1819173870159003648 |
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| author | Sandrine Lesellier Maria-Laura Boschiroli Jacques Barrat Christoph Wanke Francisco J. Salguero Waldo L. Garcia-Jimenez Alex Nunez Ana Godinho John Spiropoulos Simonette Palmer Dipesh Dave Paul Anderson Jean-Marc Boucher Krystel de Cruz Sylvie Henault Lorraine Michelet Sonya Gowtage Gareth A. Williams Allan K. Nadian Elodie Monchâtre-Leroy Frank Boué Mark A. Chambers Céline Richomme |
| author_facet | Sandrine Lesellier Maria-Laura Boschiroli Jacques Barrat Christoph Wanke Francisco J. Salguero Waldo L. Garcia-Jimenez Alex Nunez Ana Godinho John Spiropoulos Simonette Palmer Dipesh Dave Paul Anderson Jean-Marc Boucher Krystel de Cruz Sylvie Henault Lorraine Michelet Sonya Gowtage Gareth A. Williams Allan K. Nadian Elodie Monchâtre-Leroy Frank Boué Mark A. Chambers Céline Richomme |
| author_sort | Sandrine Lesellier |
| collection | DOAJ |
| description | Abstract Background Oral vaccination with Mycobacterium bovis Bacille of Calmette and Guerin (BCG) has provided protection against M. bovis to badgers both experimentally and in the field. There is also evidence suggesting that the persistence of live BCG within the host is important for maintaining protection against TB. Here we investigated the capacity of badger inductive mucosal sites to absorb and maintain live BCG. The targeted mucosae were the oropharyngeal cavity (tonsils and sublingual area) and the small intestine (ileum). Results We showed that significant quantities of live BCG persisted within badger in tissues of vaccinated badgers for at least 8 weeks following oral vaccination with only very mild pathological features and induced the circulation of IFNγ-producing mononuclear cells. The uptake of live BCG by tonsils and drainage to retro-pharyngeal lymph nodes was repeatable in the animal group vaccinated by oropharyngeal instillation whereas those vaccinated directly in the ileum displayed a lower frequency of BCG detection in the enteric wall or draining mesenteric lymph nodes. No faecal excretion of live BCG was observed, including when BCG was delivered directly in the ileum. Conclusions The apparent local loss of BCG viability suggests an unfavorable gastro-enteric environment for BCG in badgers, which should be taken in consideration when developing an oral vaccine for use in this species. |
| first_indexed | 2024-12-22T20:29:57Z |
| format | Article |
| id | doaj.art-6260306167ed4e8290269396a58341b3 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-22T20:29:57Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-6260306167ed4e8290269396a58341b32022-12-21T18:13:37ZengBMCBMC Veterinary Research1746-61482019-12-0115111510.1186/s12917-019-2166-4Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileumSandrine Lesellier0Maria-Laura Boschiroli1Jacques Barrat2Christoph Wanke3Francisco J. Salguero4Waldo L. Garcia-Jimenez5Alex Nunez6Ana Godinho7John Spiropoulos8Simonette Palmer9Dipesh Dave10Paul Anderson11Jean-Marc Boucher12Krystel de Cruz13Sylvie Henault14Lorraine Michelet15Sonya Gowtage16Gareth A. Williams17Allan K. Nadian18Elodie Monchâtre-Leroy19Frank Boué20Mark A. Chambers21Céline Richomme22Animal and Plant Health AgencyLaboratory for Animal Health, Tuberculosis National Reference Laboratory, University Paris-Est, AnsesAnses, Nancy laboratory for rabies and wildlifeMedimetrics Personalized Drug Delivery B.V.Animal and Plant Health AgencyUniversity of SurreyAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnses, Nancy laboratory for rabies and wildlifeLaboratory for Animal Health, Tuberculosis National Reference Laboratory, University Paris-Est, AnsesLaboratory for Animal Health, Tuberculosis National Reference Laboratory, University Paris-Est, AnsesLaboratory for Animal Health, Tuberculosis National Reference Laboratory, University Paris-Est, AnsesAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnimal and Plant Health AgencyAnses, Nancy laboratory for rabies and wildlifeAnses, Nancy laboratory for rabies and wildlifeAnimal and Plant Health AgencyAnses, Nancy laboratory for rabies and wildlifeAbstract Background Oral vaccination with Mycobacterium bovis Bacille of Calmette and Guerin (BCG) has provided protection against M. bovis to badgers both experimentally and in the field. There is also evidence suggesting that the persistence of live BCG within the host is important for maintaining protection against TB. Here we investigated the capacity of badger inductive mucosal sites to absorb and maintain live BCG. The targeted mucosae were the oropharyngeal cavity (tonsils and sublingual area) and the small intestine (ileum). Results We showed that significant quantities of live BCG persisted within badger in tissues of vaccinated badgers for at least 8 weeks following oral vaccination with only very mild pathological features and induced the circulation of IFNγ-producing mononuclear cells. The uptake of live BCG by tonsils and drainage to retro-pharyngeal lymph nodes was repeatable in the animal group vaccinated by oropharyngeal instillation whereas those vaccinated directly in the ileum displayed a lower frequency of BCG detection in the enteric wall or draining mesenteric lymph nodes. No faecal excretion of live BCG was observed, including when BCG was delivered directly in the ileum. Conclusions The apparent local loss of BCG viability suggests an unfavorable gastro-enteric environment for BCG in badgers, which should be taken in consideration when developing an oral vaccine for use in this species.https://doi.org/10.1186/s12917-019-2166-4BCGBadgerVaccineMucosaCapsuleTonsil |
| spellingShingle | Sandrine Lesellier Maria-Laura Boschiroli Jacques Barrat Christoph Wanke Francisco J. Salguero Waldo L. Garcia-Jimenez Alex Nunez Ana Godinho John Spiropoulos Simonette Palmer Dipesh Dave Paul Anderson Jean-Marc Boucher Krystel de Cruz Sylvie Henault Lorraine Michelet Sonya Gowtage Gareth A. Williams Allan K. Nadian Elodie Monchâtre-Leroy Frank Boué Mark A. Chambers Céline Richomme Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum BMC Veterinary Research BCG Badger Vaccine Mucosa Capsule Tonsil |
| title | Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum |
| title_full | Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum |
| title_fullStr | Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum |
| title_full_unstemmed | Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum |
| title_short | Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum |
| title_sort | detection of live m bovis bcg in tissues and ifn γ responses in european badgers meles meles vaccinated by oropharyngeal instillation or directly in the ileum |
| topic | BCG Badger Vaccine Mucosa Capsule Tonsil |
| url | https://doi.org/10.1186/s12917-019-2166-4 |
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