Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system
Abstract The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In thi...
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| Format: | Article |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82412-z |
| _version_ | 1818846175606865920 |
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| author | M. Ashokan Kerekoppa P. Ramesha Sweta Hallur Gayathree Karthikkeyan Ekta Rana N. Azharuddin S. Reshma Raj S. Jeyakumar A. Kumaresan Mukund A. Kataktalware D. N. Das T. S. Keshava Prasad |
| author_facet | M. Ashokan Kerekoppa P. Ramesha Sweta Hallur Gayathree Karthikkeyan Ekta Rana N. Azharuddin S. Reshma Raj S. Jeyakumar A. Kumaresan Mukund A. Kataktalware D. N. Das T. S. Keshava Prasad |
| author_sort | M. Ashokan |
| collection | DOAJ |
| description | Abstract The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In this study, we used acetonitrile precipitation and methanol:chloroform methods for extracting the milk metabolites followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) run to identify the different metabolites between the milk of grazing and non-grazing early lactating Malnad Gidda cows. Various carbohydrates, amino acids, nucleosides and vitamin derivatives were found to be differentially abundant in grazing cows. A total of 35 metabolites were differentially regulated (fold change above 1.5) between the two groups. Tyrosyl-threonine, histidinyl-cysteine, 1-methyladenine, l-cysteine and selenocysteine showed fold change above 3 in grazing cows. The lipid profile of milk showed a lesser difference between grazing and non-grazing cows as compared to polar metabolites. To the best of our knowledge, this is the largest inventory of milk metabolomics data of an Indian cattle (Bos indicus) breed. We believe that our study would help to emerge a field of Nutri-metabolomics and veterinary omics research. |
| first_indexed | 2024-12-19T05:41:23Z |
| format | Article |
| id | doaj.art-6261de6585ac46859187ecfec5a50ea8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:41:23Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6261de6585ac46859187ecfec5a50ea82022-12-21T20:34:00ZengNature PortfolioScientific Reports2045-23222021-02-0111111310.1038/s41598-021-82412-zDifferences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding systemM. Ashokan0Kerekoppa P. Ramesha1Sweta Hallur2Gayathree Karthikkeyan3Ekta Rana4N. Azharuddin5S. Reshma Raj6S. Jeyakumar7A. Kumaresan8Mukund A. Kataktalware9D. N. Das10T. S. Keshava Prasad11Southern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteCenter for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University)Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University)Southern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteSouthern Regional Station, ICAR-National Dairy Research InstituteCenter for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University)Abstract The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In this study, we used acetonitrile precipitation and methanol:chloroform methods for extracting the milk metabolites followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) run to identify the different metabolites between the milk of grazing and non-grazing early lactating Malnad Gidda cows. Various carbohydrates, amino acids, nucleosides and vitamin derivatives were found to be differentially abundant in grazing cows. A total of 35 metabolites were differentially regulated (fold change above 1.5) between the two groups. Tyrosyl-threonine, histidinyl-cysteine, 1-methyladenine, l-cysteine and selenocysteine showed fold change above 3 in grazing cows. The lipid profile of milk showed a lesser difference between grazing and non-grazing cows as compared to polar metabolites. To the best of our knowledge, this is the largest inventory of milk metabolomics data of an Indian cattle (Bos indicus) breed. We believe that our study would help to emerge a field of Nutri-metabolomics and veterinary omics research.https://doi.org/10.1038/s41598-021-82412-z |
| spellingShingle | M. Ashokan Kerekoppa P. Ramesha Sweta Hallur Gayathree Karthikkeyan Ekta Rana N. Azharuddin S. Reshma Raj S. Jeyakumar A. Kumaresan Mukund A. Kataktalware D. N. Das T. S. Keshava Prasad Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system Scientific Reports |
| title | Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_full | Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_fullStr | Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_full_unstemmed | Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_short | Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_sort | differences in milk metabolites in malnad gidda bos indicus cows reared under pasture based feeding system |
| url | https://doi.org/10.1038/s41598-021-82412-z |
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