DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans
The study evaluated the association of DNase I enzyme with antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in mice infected with fluconazole-susceptible (CaS) and -resistant (CaR) Candida albicans strains. Mice were inoculated with C. albicans, and after the infection...
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| Format: | Article |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1274201/full |
| _version_ | 1797382992606265344 |
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| author | Cláudia Carolina Jordão Marlise Inêz Klein Paula Aboud Barbugli Ewerton Garcia de Oliveira Mima Tábata Viana de Sousa Túlio Morandin Ferrisse Ana Claudia Pavarina |
| author_facet | Cláudia Carolina Jordão Marlise Inêz Klein Paula Aboud Barbugli Ewerton Garcia de Oliveira Mima Tábata Viana de Sousa Túlio Morandin Ferrisse Ana Claudia Pavarina |
| author_sort | Cláudia Carolina Jordão |
| collection | DOAJ |
| description | The study evaluated the association of DNase I enzyme with antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in mice infected with fluconazole-susceptible (CaS) and -resistant (CaR) Candida albicans strains. Mice were inoculated with C. albicans, and after the infection had been established, the tongues were exposed to DNase for 5 min, followed by photosensitizer [Photodithazine®(PDZ)] and light (LED), either singly or combined. The treatments were performed for 5 consecutive days. Treatment efficacy was evaluated by assessing the tongues via fungal viable population, clinical evaluation, histopathological and fluorescence microscopy methods immediately after finishing treatments, and 7 days of follow-up. The combination of DNase with PDZ-aPDT reduced the fungal viability in mice tongues immediately after the treatments by around 4.26 and 2.89 log10 for CaS and CaR, respectively (versus animals only inoculated). In the fluorescence microscopy, the polysaccharides produced by C. albicans and fungal cells were less labeled in animals treated with the combination of DNase with PDZ-aPDT, similar to the healthy animals. After 7 days of the treatment, DNase associated with PDZ-aPDT maintained a lower count, but not as pronounced as immediately after the intervention. For both strains, mice treated with the combination of DNase with PDZ-aPDT showed remission of oral lesions and mild inflammatory infiltrate in both periods assessed, while animals treated only with PDZ-aPDT presented partial remission of oral lesions. DNase I enzyme improved the efficacy of photodynamic treatment. |
| first_indexed | 2024-03-08T21:13:21Z |
| format | Article |
| id | doaj.art-62623b275b0b4e84973f079e3a68c991 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T21:13:21Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-62623b275b0b4e84973f079e3a68c9912023-12-22T04:20:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.12742011274201DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicansCláudia Carolina Jordão0Marlise Inêz Klein1Paula Aboud Barbugli2Ewerton Garcia de Oliveira Mima3Tábata Viana de Sousa4Túlio Morandin Ferrisse5Ana Claudia Pavarina6Laboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, BrazilDepartment of Oral Diagnosis, Piracicaba Dental School, State University of Campinas (UNICAMP), Piracicaba, BrazilLaboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, BrazilLaboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, BrazilLaboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, BrazilLaboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, BrazilLaboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, BrazilThe study evaluated the association of DNase I enzyme with antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in mice infected with fluconazole-susceptible (CaS) and -resistant (CaR) Candida albicans strains. Mice were inoculated with C. albicans, and after the infection had been established, the tongues were exposed to DNase for 5 min, followed by photosensitizer [Photodithazine®(PDZ)] and light (LED), either singly or combined. The treatments were performed for 5 consecutive days. Treatment efficacy was evaluated by assessing the tongues via fungal viable population, clinical evaluation, histopathological and fluorescence microscopy methods immediately after finishing treatments, and 7 days of follow-up. The combination of DNase with PDZ-aPDT reduced the fungal viability in mice tongues immediately after the treatments by around 4.26 and 2.89 log10 for CaS and CaR, respectively (versus animals only inoculated). In the fluorescence microscopy, the polysaccharides produced by C. albicans and fungal cells were less labeled in animals treated with the combination of DNase with PDZ-aPDT, similar to the healthy animals. After 7 days of the treatment, DNase associated with PDZ-aPDT maintained a lower count, but not as pronounced as immediately after the intervention. For both strains, mice treated with the combination of DNase with PDZ-aPDT showed remission of oral lesions and mild inflammatory infiltrate in both periods assessed, while animals treated only with PDZ-aPDT presented partial remission of oral lesions. DNase I enzyme improved the efficacy of photodynamic treatment.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1274201/fullphotochemotherapyCandida albicansantifungal drug resistancefungienzyme |
| spellingShingle | Cláudia Carolina Jordão Marlise Inêz Klein Paula Aboud Barbugli Ewerton Garcia de Oliveira Mima Tábata Viana de Sousa Túlio Morandin Ferrisse Ana Claudia Pavarina DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans Frontiers in Microbiology photochemotherapy Candida albicans antifungal drug resistance fungi enzyme |
| title | DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans |
| title_full | DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans |
| title_fullStr | DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans |
| title_full_unstemmed | DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans |
| title_short | DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans |
| title_sort | dnase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with candida albicans |
| topic | photochemotherapy Candida albicans antifungal drug resistance fungi enzyme |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1274201/full |
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