Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium
Objective: Eudistoma kaverium, an ascidian belonging to the family Polycitoridae is previously recorded from Indian waters. The work done on this species is limited only to taxonomy and ecology. As ascidians are potential source of anticancer compounds, we have purified fractions of E. kaverium usin...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721000793 |
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| author | Yasrib Qurishi Vidya Devanathadesikan Seshadri Mohammed Mustafa Poyil Jeyaseelan Benjamin Franklin Deepak Arun Apte Mohammed H. Karrar Alsharif Rajaian Pushpabai Rajesh Randa Mohammed Zaki |
| author_facet | Yasrib Qurishi Vidya Devanathadesikan Seshadri Mohammed Mustafa Poyil Jeyaseelan Benjamin Franklin Deepak Arun Apte Mohammed H. Karrar Alsharif Rajaian Pushpabai Rajesh Randa Mohammed Zaki |
| author_sort | Yasrib Qurishi |
| collection | DOAJ |
| description | Objective: Eudistoma kaverium, an ascidian belonging to the family Polycitoridae is previously recorded from Indian waters. The work done on this species is limited only to taxonomy and ecology. As ascidians are potential source of anticancer compounds, we have purified fractions of E. kaverium using HPLC and evaluated its anticancer activity. Method: Chromatography based fractionation of the crude extract was carried out and mass spectrometry was used to identify the active molecule. Cell viability was checked using MTT assay. Flow cytometry, ROS generation, Hoechst staining and DNA fragmentation was performed to confirm the anticancer activity of the compound. Results: A column fraction EK-7, which was identified as a sterol Cholesta-4,6-dien-3-ol using GC–MS analysis, exhibited potent cytotoxicity with IC50 value of 6.5 μM in MCF-7 and 10.2 μM against HeLa cells. Moreover, EK-7 triggered the cell cycle arrest in the sub G0/G1 stage and induced apoptosis twenty-four hours post-treatment. Hoechst staining of EK-7-treated HeLa cells portrayed apoptotic events such as changes in cell morphology, chromatin condensation, membrane swelling, and development of apoptotic bodies. The changes in light scattering by the EK-7-treated HeLa cells indicate the general characteristics of cell death as a result of apoptosis. The EK-7-treated HeLa cells show cell cycle arrest in G0/G1, G2/M and S phases and the increased amount of sub G0/G1 population indicates the increase in the apoptotic induced cell population. The isolated compound Cholesta-4,6-dien-3-ol (EK-7), based on the GC–MS analysis and library search from E. kaverium displayed potent anticancer activity which is mediated through apoptosis targeting the G0/G1 phase. Conclusion: Based on the experimental data, it shows that EK-7(Cholesta-4,6-dien-3-ol) a sterol derivative has potent anticancer activity mediated through apoptosis. If this research is continued further with preclinical and clinical trials, EK-7(Cholesta-4,6-dien-3-ol) could be possibly used as a chemotherapeutic agent in treating cancer. |
| first_indexed | 2024-12-17T22:57:58Z |
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| language | English |
| last_indexed | 2024-12-17T22:57:58Z |
| publishDate | 2021-06-01 |
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| series | Journal of King Saud University: Science |
| spelling | doaj.art-626393778a7f44ea845beb52576e9fde2022-12-21T21:29:29ZengElsevierJournal of King Saud University: Science1018-36472021-06-01334101418Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaveriumYasrib Qurishi0Vidya Devanathadesikan Seshadri1Mohammed Mustafa Poyil2Jeyaseelan Benjamin Franklin3Deepak Arun Apte4Mohammed H. Karrar Alsharif5Rajaian Pushpabai Rajesh6Randa Mohammed Zaki7Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, Karnataka, IndiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam bin Abdul Aziz University, Al Kharj, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdul Aziz University, Al Kharj, Saudi ArabiaBombay Natural History Society, Hornbill House, Dr. Salim Ali Chowk, Mumbai, 400001, IndiaBombay Natural History Society, Hornbill House, Dr. Salim Ali Chowk, Mumbai, 400001, IndiaDepartment of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdul Aziz University, Al Kharj, Saudi ArabiaCenter for Molecular and Nanomedical Sciences, Sathyabama Institute of Science and Technology, Chennai, India; Corresponding author.Department of Pharmaceutics, Faculty of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi ArabiaObjective: Eudistoma kaverium, an ascidian belonging to the family Polycitoridae is previously recorded from Indian waters. The work done on this species is limited only to taxonomy and ecology. As ascidians are potential source of anticancer compounds, we have purified fractions of E. kaverium using HPLC and evaluated its anticancer activity. Method: Chromatography based fractionation of the crude extract was carried out and mass spectrometry was used to identify the active molecule. Cell viability was checked using MTT assay. Flow cytometry, ROS generation, Hoechst staining and DNA fragmentation was performed to confirm the anticancer activity of the compound. Results: A column fraction EK-7, which was identified as a sterol Cholesta-4,6-dien-3-ol using GC–MS analysis, exhibited potent cytotoxicity with IC50 value of 6.5 μM in MCF-7 and 10.2 μM against HeLa cells. Moreover, EK-7 triggered the cell cycle arrest in the sub G0/G1 stage and induced apoptosis twenty-four hours post-treatment. Hoechst staining of EK-7-treated HeLa cells portrayed apoptotic events such as changes in cell morphology, chromatin condensation, membrane swelling, and development of apoptotic bodies. The changes in light scattering by the EK-7-treated HeLa cells indicate the general characteristics of cell death as a result of apoptosis. The EK-7-treated HeLa cells show cell cycle arrest in G0/G1, G2/M and S phases and the increased amount of sub G0/G1 population indicates the increase in the apoptotic induced cell population. The isolated compound Cholesta-4,6-dien-3-ol (EK-7), based on the GC–MS analysis and library search from E. kaverium displayed potent anticancer activity which is mediated through apoptosis targeting the G0/G1 phase. Conclusion: Based on the experimental data, it shows that EK-7(Cholesta-4,6-dien-3-ol) a sterol derivative has potent anticancer activity mediated through apoptosis. If this research is continued further with preclinical and clinical trials, EK-7(Cholesta-4,6-dien-3-ol) could be possibly used as a chemotherapeutic agent in treating cancer.http://www.sciencedirect.com/science/article/pii/S1018364721000793Eudistoma kaveriumAscidianHeLaMTTFlow cytometryApoptosis |
| spellingShingle | Yasrib Qurishi Vidya Devanathadesikan Seshadri Mohammed Mustafa Poyil Jeyaseelan Benjamin Franklin Deepak Arun Apte Mohammed H. Karrar Alsharif Rajaian Pushpabai Rajesh Randa Mohammed Zaki Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium Journal of King Saud University: Science Eudistoma kaverium Ascidian HeLa MTT Flow cytometry Apoptosis |
| title | Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium |
| title_full | Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium |
| title_fullStr | Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium |
| title_full_unstemmed | Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium |
| title_short | Anticancer activity in HeLa and MCF-7 cells via apoptopic cell death by a sterol molecule Cholesta-4,6-dien-3-ol (EK-7), from the marine ascidian Eudistoma kaverium |
| title_sort | anticancer activity in hela and mcf 7 cells via apoptopic cell death by a sterol molecule cholesta 4 6 dien 3 ol ek 7 from the marine ascidian eudistoma kaverium |
| topic | Eudistoma kaverium Ascidian HeLa MTT Flow cytometry Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721000793 |
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