d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae
Abstract Rapid and effective consumption of d-xylose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. Hence, heterologous d-xylose metabolic pathways have been introduced into S. cerevisiae. An effective solution is based on a xylose isomerase in combinat...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02320-4 |
| _version_ | 1797841145643925504 |
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| author | Jeroen G. Nijland Xiaohuan Zhang Arnold J. M. Driessen |
| author_facet | Jeroen G. Nijland Xiaohuan Zhang Arnold J. M. Driessen |
| author_sort | Jeroen G. Nijland |
| collection | DOAJ |
| description | Abstract Rapid and effective consumption of d-xylose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. Hence, heterologous d-xylose metabolic pathways have been introduced into S. cerevisiae. An effective solution is based on a xylose isomerase in combination with the overexpression of the xylulose kinase (Xks1) and all genes of the non-oxidative branch of the pentose phosphate pathway. Although this strain is capable of consuming d-xylose, growth inhibition occurs at higher d-xylose concentrations, even abolishing growth completely at 8% d-xylose. The decreased growth rates are accompanied by significantly decreased ATP levels. A key ATP-utilizing step in d-xylose metabolism is the phosphorylation of d-xylulose by Xks1. Replacement of the constitutive promoter of XKS1 by the galactose tunable promoter Pgal10 allowed the controlled expression of this gene over a broad range. By decreasing the expression levels of XKS1, growth at high d-xylose concentrations could be restored concomitantly with increased ATP levels and high rates of xylose metabolism. These data show that in fermentations with high d-xylose concentrations, too high levels of Xks1 cause a major drain on the cellular ATP levels thereby reducing the growth rate, ultimately causing substrate accelerated death. Hence, expression levels of XKS1 in S. cerevisiae needs to be tailored for the specific growth conditions and robust d-xylose metabolism. |
| first_indexed | 2024-04-09T16:26:20Z |
| format | Article |
| id | doaj.art-62652926ce584f378e80877316a6481c |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-09T16:26:20Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-62652926ce584f378e80877316a6481c2023-04-23T11:11:47ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-04-0116111010.1186/s13068-023-02320-4d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiaeJeroen G. Nijland0Xiaohuan Zhang1Arnold J. M. Driessen2Molecular Microbiology, Groningen Biomolecular Sciences and BiotechnologyMolecular Microbiology, Groningen Biomolecular Sciences and BiotechnologyMolecular Microbiology, Groningen Biomolecular Sciences and BiotechnologyAbstract Rapid and effective consumption of d-xylose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. Hence, heterologous d-xylose metabolic pathways have been introduced into S. cerevisiae. An effective solution is based on a xylose isomerase in combination with the overexpression of the xylulose kinase (Xks1) and all genes of the non-oxidative branch of the pentose phosphate pathway. Although this strain is capable of consuming d-xylose, growth inhibition occurs at higher d-xylose concentrations, even abolishing growth completely at 8% d-xylose. The decreased growth rates are accompanied by significantly decreased ATP levels. A key ATP-utilizing step in d-xylose metabolism is the phosphorylation of d-xylulose by Xks1. Replacement of the constitutive promoter of XKS1 by the galactose tunable promoter Pgal10 allowed the controlled expression of this gene over a broad range. By decreasing the expression levels of XKS1, growth at high d-xylose concentrations could be restored concomitantly with increased ATP levels and high rates of xylose metabolism. These data show that in fermentations with high d-xylose concentrations, too high levels of Xks1 cause a major drain on the cellular ATP levels thereby reducing the growth rate, ultimately causing substrate accelerated death. Hence, expression levels of XKS1 in S. cerevisiae needs to be tailored for the specific growth conditions and robust d-xylose metabolism.https://doi.org/10.1186/s13068-023-02320-4d-xylose consumptionATPXks1 expressionBioethanolYeast |
| spellingShingle | Jeroen G. Nijland Xiaohuan Zhang Arnold J. M. Driessen d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae Biotechnology for Biofuels and Bioproducts d-xylose consumption ATP Xks1 expression Bioethanol Yeast |
| title | d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae |
| title_full | d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae |
| title_fullStr | d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae |
| title_full_unstemmed | d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae |
| title_short | d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae |
| title_sort | d xylose accelerated death of pentose metabolizing saccharomyces cerevisiae |
| topic | d-xylose consumption ATP Xks1 expression Bioethanol Yeast |
| url | https://doi.org/10.1186/s13068-023-02320-4 |
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