Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.)
Anthocyanin is an important pigment that affects plant color and nutritional quality. MYBs play an important role in plant anthocyanin synthesis and accumulation. However, the regulatory function of MYB transcription factors in anthocyanin synthesis in flax flowers is still unclear. In this study, 4...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923001168 |
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| author | Dong-wei XIE Jing LI Xiao-yu ZHANG Zhi-gang DAI Wen-zhi ZHOU Jian-guang SU Jian SUN |
| author_facet | Dong-wei XIE Jing LI Xiao-yu ZHANG Zhi-gang DAI Wen-zhi ZHOU Jian-guang SU Jian SUN |
| author_sort | Dong-wei XIE |
| collection | DOAJ |
| description | Anthocyanin is an important pigment that affects plant color and nutritional quality. MYBs play an important role in plant anthocyanin synthesis and accumulation. However, the regulatory function of MYB transcription factors in anthocyanin synthesis in flax flowers is still unclear. In this study, 402 MYB transcription factors were identified in the flax genome. These MYB members are unevenly distributed on 15 chromosomes. The R2R3-LuMYB members were divided into 32 phylogenetic subfamilies. qRT-PCR analysis showed that seven R2R3-LuMYB genes in the adjacent subfamily of the evolutionary tree had similar expression patterns, among which LuMYB216 was highly expressed in the petals of different colors. Moreover, gene editing of LuMYB216 in flax showed that the petal color, anther color and seed coat color of mutant plants were significantly lighter than those of wild-type plants, and the anthocyanin content of lumyb216 mutant plants was significantly reduced. Correlation analysis indicated that LuMYB216 was significantly positively correlated with the upstream regulator bHLH30. This study systematically analyzed the MYB gene family in flax, laying a foundation for studying the regulation of LuMYB216 in flax flower anthocyanin synthesis. |
| first_indexed | 2024-03-12T15:04:20Z |
| format | Article |
| id | doaj.art-6269e111448c49a08cd1c2faaa4426b1 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-12T15:04:20Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-6269e111448c49a08cd1c2faaa4426b12023-08-13T04:53:34ZengElsevierJournal of Integrative Agriculture2095-31192023-08-0122823352345Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.)Dong-wei XIE0Jing LI1Xiao-yu ZHANG2Zhi-gang DAI3Wen-zhi ZHOU4Jian-guang SU5Jian SUN6School of Life Science, Nantong University, Nantong 226019, P.R.ChinaSchool of Life Science, Nantong University, Nantong 226019, P.R.ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, P.R.ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, P.R.ChinaJiangsu Sanshu Biotechnology Co., Ltd., Nantong 226001, P.R.ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, P.R.ChinaSchool of Life Science, Nantong University, Nantong 226019, P.R.China; Correspondence SUN Jian, Tel: +86-513-85012818Anthocyanin is an important pigment that affects plant color and nutritional quality. MYBs play an important role in plant anthocyanin synthesis and accumulation. However, the regulatory function of MYB transcription factors in anthocyanin synthesis in flax flowers is still unclear. In this study, 402 MYB transcription factors were identified in the flax genome. These MYB members are unevenly distributed on 15 chromosomes. The R2R3-LuMYB members were divided into 32 phylogenetic subfamilies. qRT-PCR analysis showed that seven R2R3-LuMYB genes in the adjacent subfamily of the evolutionary tree had similar expression patterns, among which LuMYB216 was highly expressed in the petals of different colors. Moreover, gene editing of LuMYB216 in flax showed that the petal color, anther color and seed coat color of mutant plants were significantly lighter than those of wild-type plants, and the anthocyanin content of lumyb216 mutant plants was significantly reduced. Correlation analysis indicated that LuMYB216 was significantly positively correlated with the upstream regulator bHLH30. This study systematically analyzed the MYB gene family in flax, laying a foundation for studying the regulation of LuMYB216 in flax flower anthocyanin synthesis.http://www.sciencedirect.com/science/article/pii/S2095311923001168flaxMYB gene familyanthocyanin biosynthesisLuMYB216functional characterization |
| spellingShingle | Dong-wei XIE Jing LI Xiao-yu ZHANG Zhi-gang DAI Wen-zhi ZHOU Jian-guang SU Jian SUN Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.) Journal of Integrative Agriculture flax MYB gene family anthocyanin biosynthesis LuMYB216 functional characterization |
| title | Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.) |
| title_full | Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.) |
| title_fullStr | Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.) |
| title_full_unstemmed | Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.) |
| title_short | Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.) |
| title_sort | systematic analysis of myb transcription factors and the role of lumyb216 in regulating anthocyanin biosynthesis in the flowers of flax linum usitatissimum l |
| topic | flax MYB gene family anthocyanin biosynthesis LuMYB216 functional characterization |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923001168 |
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