Novel insights into the transcriptome of Dirofilaria immitis.
BACKGROUND: The heartworm Dirofilaria immitis is the causal agent of cardiopulmonary dirofilariosis in dogs and cats, and also infects a wide range of wild mammals as well as humans. One bottleneck for the design of fundamentally new intervention and management strategies against D. immitis may be t...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3402454?pdf=render |
| _version_ | 1818153326142816256 |
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| author | Yan Fu Jingchao Lan Zhihe Zhang Rong Hou Xuhang Wu Deying Yang Runhui Zhang Wanpeng Zheng Huaming Nie Yue Xie Ning Yan Zhi Yang Chengdong Wang Li Luo Li Liu Xiaobin Gu Shuxian Wang Xuerong Peng Guangyou Yang |
| author_facet | Yan Fu Jingchao Lan Zhihe Zhang Rong Hou Xuhang Wu Deying Yang Runhui Zhang Wanpeng Zheng Huaming Nie Yue Xie Ning Yan Zhi Yang Chengdong Wang Li Luo Li Liu Xiaobin Gu Shuxian Wang Xuerong Peng Guangyou Yang |
| author_sort | Yan Fu |
| collection | DOAJ |
| description | BACKGROUND: The heartworm Dirofilaria immitis is the causal agent of cardiopulmonary dirofilariosis in dogs and cats, and also infects a wide range of wild mammals as well as humans. One bottleneck for the design of fundamentally new intervention and management strategies against D. immitis may be the currently limited knowledge of fundamental molecular aspects of D. immitis. METHODOLOGY/PRINCIPAL FINDINGS: A next-generation sequencing platform combining computational approaches was employed to assess a global view of the heartworm transcriptome. A total of 20,810 unigenes (mean length = 1,270 bp) were assembled from 22.3 million clean reads. From these, 15,698 coding sequences (CDS) were inferred, and about 85% of the unigenes had orthologs/homologs in public databases. Comparative transcriptomic study uncovered 4,157 filarial-specific genes as well as 3,795 genes potentially involved in filarial-Wolbachia symbiosis. In addition, the potential intestine transcriptome of D. immitis (1,101 genes) was mined for the first time, which might help to discover 'hidden antigens'. CONCLUSIONS/SIGNIFICANCE: This study provides novel insights into the transcriptome of D. immitis and sheds light on its molecular processes and survival mechanisms. Furthermore, it provides a platform to discover new vaccine candidates and potential targets for new drugs against dirofilariosis. |
| first_indexed | 2024-12-11T14:08:50Z |
| format | Article |
| id | doaj.art-626abf4478d3444885df6f6ac12b4de1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T14:08:50Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-626abf4478d3444885df6f6ac12b4de12022-12-22T01:03:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4163910.1371/journal.pone.0041639Novel insights into the transcriptome of Dirofilaria immitis.Yan FuJingchao LanZhihe ZhangRong HouXuhang WuDeying YangRunhui ZhangWanpeng ZhengHuaming NieYue XieNing YanZhi YangChengdong WangLi LuoLi LiuXiaobin GuShuxian WangXuerong PengGuangyou YangBACKGROUND: The heartworm Dirofilaria immitis is the causal agent of cardiopulmonary dirofilariosis in dogs and cats, and also infects a wide range of wild mammals as well as humans. One bottleneck for the design of fundamentally new intervention and management strategies against D. immitis may be the currently limited knowledge of fundamental molecular aspects of D. immitis. METHODOLOGY/PRINCIPAL FINDINGS: A next-generation sequencing platform combining computational approaches was employed to assess a global view of the heartworm transcriptome. A total of 20,810 unigenes (mean length = 1,270 bp) were assembled from 22.3 million clean reads. From these, 15,698 coding sequences (CDS) were inferred, and about 85% of the unigenes had orthologs/homologs in public databases. Comparative transcriptomic study uncovered 4,157 filarial-specific genes as well as 3,795 genes potentially involved in filarial-Wolbachia symbiosis. In addition, the potential intestine transcriptome of D. immitis (1,101 genes) was mined for the first time, which might help to discover 'hidden antigens'. CONCLUSIONS/SIGNIFICANCE: This study provides novel insights into the transcriptome of D. immitis and sheds light on its molecular processes and survival mechanisms. Furthermore, it provides a platform to discover new vaccine candidates and potential targets for new drugs against dirofilariosis.http://europepmc.org/articles/PMC3402454?pdf=render |
| spellingShingle | Yan Fu Jingchao Lan Zhihe Zhang Rong Hou Xuhang Wu Deying Yang Runhui Zhang Wanpeng Zheng Huaming Nie Yue Xie Ning Yan Zhi Yang Chengdong Wang Li Luo Li Liu Xiaobin Gu Shuxian Wang Xuerong Peng Guangyou Yang Novel insights into the transcriptome of Dirofilaria immitis. PLoS ONE |
| title | Novel insights into the transcriptome of Dirofilaria immitis. |
| title_full | Novel insights into the transcriptome of Dirofilaria immitis. |
| title_fullStr | Novel insights into the transcriptome of Dirofilaria immitis. |
| title_full_unstemmed | Novel insights into the transcriptome of Dirofilaria immitis. |
| title_short | Novel insights into the transcriptome of Dirofilaria immitis. |
| title_sort | novel insights into the transcriptome of dirofilaria immitis |
| url | http://europepmc.org/articles/PMC3402454?pdf=render |
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