Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings
Abstract Introduction Available human papillomavirus (HPV) vaccines could have an important primary role in cervical cancer prevention once their long-term immunogenicity and safety are evaluated at the population level. The aim of this study was to optimize an assay to be used in evaluating the lon...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Women's Health |
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| Online Access: | https://doi.org/10.1186/s12905-022-01821-y |
| _version_ | 1818228291266412544 |
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| author | Emmanuel Timmy Donkoh Edward Tieru Dassah Ellis Owusu-Dabo |
| author_facet | Emmanuel Timmy Donkoh Edward Tieru Dassah Ellis Owusu-Dabo |
| author_sort | Emmanuel Timmy Donkoh |
| collection | DOAJ |
| description | Abstract Introduction Available human papillomavirus (HPV) vaccines could have an important primary role in cervical cancer prevention once their long-term immunogenicity and safety are evaluated at the population level. The aim of this study was to optimize an assay to be used in evaluating the long-term durability of HPV vaccine response following a pilot vaccination of adolescent girls in Ghana. Methods A rapid, high-throughput, indirect enzyme-linked immunosorbent assay (ELISA) was optimized for the detection and quantitation of anti-HPV L1 (late expression protein: types 6, 11, 16 and 18) immunoglobulin G (IgG) in human serum (n = 89). The utility of the assay was demonstrated using serum collected from a cohort of pre-adolescent girls (n = 49) previously vaccinated with a quadrivalent vaccine and non-immune serum obtained from age-matched controls (n = 40). Results The assay showed good discrimination of antibody levels between cases and control sera: seroprevalence of anti-HPV IgG antibodies was significantly higher among vaccinated than unvaccinated girls for both HPV-16 (63.3% vs. 12.5%; p < 0.001) and HPV-18 (34.7% vs. 20.0%; p = 0.042), respectively. Thirty-six months after receiving the third dose of vaccine, significantly higher mean anti-HPV-16 (0.618 vs. 0.145), anti-HPV-18 (0.323 vs. 0.309), and anti-HPV-6 (1.371 vs. 0.981) antibody levels were measured, compared to unvaccinated girls (all p < 0.05). A correlation between optical density and antibody activity indicated assay sensitivity to increasing levels of antibody activity. Conclusion We have successfully optimized and implemented a robust and sensitive assay for the evaluation of antibody responses among immunized adolescent girls for monitoring future large-scale HPV vaccination studies in low-income settings. Our results demonstrated greater immunoglobulin G antibody activity within serum drawn from adolescent girls immunized 36 months prior. |
| first_indexed | 2024-12-12T10:00:22Z |
| format | Article |
| id | doaj.art-626e2518968b4bf9ae6772995eb0737a |
| institution | Directory Open Access Journal |
| issn | 1472-6874 |
| language | English |
| last_indexed | 2024-12-12T10:00:22Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Women's Health |
| spelling | doaj.art-626e2518968b4bf9ae6772995eb0737a2022-12-22T00:28:01ZengBMCBMC Women's Health1472-68742022-06-012211910.1186/s12905-022-01821-yOptimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settingsEmmanuel Timmy Donkoh0Edward Tieru Dassah1Ellis Owusu-Dabo2Center for Research in Applied Biology, University of Energy and Natural ResourcesDepartment of Population, Family and Reproductive Health, School of Public Health, Kwame Nkrumah University of Science and TechnologyDepartment of Global and International Health, School of Public Health, Kwame Nkrumah University of Science and TechnologyAbstract Introduction Available human papillomavirus (HPV) vaccines could have an important primary role in cervical cancer prevention once their long-term immunogenicity and safety are evaluated at the population level. The aim of this study was to optimize an assay to be used in evaluating the long-term durability of HPV vaccine response following a pilot vaccination of adolescent girls in Ghana. Methods A rapid, high-throughput, indirect enzyme-linked immunosorbent assay (ELISA) was optimized for the detection and quantitation of anti-HPV L1 (late expression protein: types 6, 11, 16 and 18) immunoglobulin G (IgG) in human serum (n = 89). The utility of the assay was demonstrated using serum collected from a cohort of pre-adolescent girls (n = 49) previously vaccinated with a quadrivalent vaccine and non-immune serum obtained from age-matched controls (n = 40). Results The assay showed good discrimination of antibody levels between cases and control sera: seroprevalence of anti-HPV IgG antibodies was significantly higher among vaccinated than unvaccinated girls for both HPV-16 (63.3% vs. 12.5%; p < 0.001) and HPV-18 (34.7% vs. 20.0%; p = 0.042), respectively. Thirty-six months after receiving the third dose of vaccine, significantly higher mean anti-HPV-16 (0.618 vs. 0.145), anti-HPV-18 (0.323 vs. 0.309), and anti-HPV-6 (1.371 vs. 0.981) antibody levels were measured, compared to unvaccinated girls (all p < 0.05). A correlation between optical density and antibody activity indicated assay sensitivity to increasing levels of antibody activity. Conclusion We have successfully optimized and implemented a robust and sensitive assay for the evaluation of antibody responses among immunized adolescent girls for monitoring future large-scale HPV vaccination studies in low-income settings. Our results demonstrated greater immunoglobulin G antibody activity within serum drawn from adolescent girls immunized 36 months prior.https://doi.org/10.1186/s12905-022-01821-yHPVQuadrivalent vaccineAntibodiesImmunogenicityGhana |
| spellingShingle | Emmanuel Timmy Donkoh Edward Tieru Dassah Ellis Owusu-Dabo Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings BMC Women's Health HPV Quadrivalent vaccine Antibodies Immunogenicity Ghana |
| title | Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings |
| title_full | Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings |
| title_fullStr | Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings |
| title_full_unstemmed | Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings |
| title_short | Optimization of a protocol for the evaluation of antibody responses to human papillomavirus (HPV) vaccination in low-resource settings |
| title_sort | optimization of a protocol for the evaluation of antibody responses to human papillomavirus hpv vaccination in low resource settings |
| topic | HPV Quadrivalent vaccine Antibodies Immunogenicity Ghana |
| url | https://doi.org/10.1186/s12905-022-01821-y |
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