Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines
Wolbachia, an intracellular maternally transmitted endosymbiont, has been shown to interfere with the replication of dengue virus in Aedes aegypti mosquitoes. The Wolbachia-transinfected Ae. aegypti has been currently released in many countries to test its effectiveness in preventing the transmissio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.947857/full |
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| author | Candasamy Sadanandane Kasinathan Gunasekaran Devaraju Panneer Sarala K. Subbarao Manju Rahi Balakrishnan Vijayakumar Velan Athithan Annamalai Sakthivel Sundaram Dinesh Purushothaman Jambulingam |
| author_facet | Candasamy Sadanandane Kasinathan Gunasekaran Devaraju Panneer Sarala K. Subbarao Manju Rahi Balakrishnan Vijayakumar Velan Athithan Annamalai Sakthivel Sundaram Dinesh Purushothaman Jambulingam |
| author_sort | Candasamy Sadanandane |
| collection | DOAJ |
| description | Wolbachia, an intracellular maternally transmitted endosymbiont, has been shown to interfere with the replication of dengue virus in Aedes aegypti mosquitoes. The Wolbachia-transinfected Ae. aegypti has been currently released in many countries to test its effectiveness in preventing the transmission of dengue virus. ICMR-Vector Control Research Centre in collaboration with World Mosquito Program Monash University, Australia, has generated two new Wolbachia-introgressed Ae. aegypti Puducherry (Pud) lines via backcrossing Ae. aegypti females of Australian (Aus) strains, infected with wMel and wAlbB Wolbachia with wild-type Ae. aegypti Puducherry (Pud) males. Wolbachia infections are known to induce a fitness cost and confer benefit on the host mosquito populations that will influence spread of the Wolbachia into native wild mosquito populations during the field release. Hence, the induced fitness cost or benefit/advantage in the two newly generated Ae. aegypti (Pud) lines was assessed in the laboratory in comparison with the wild-type Ae. aegypti (Pud) strain. In addition, maternal transmission (MT) efficiency, induced cytoplasmic incompatibility (CI), and insecticide resistance status of the two (Pud) lines were determined to assess the likely frequency of wMel and wAlbB infections in the native wild population after field invasion. The study shows that wMel and wAlbB infections did not induce any fitness cost on the two newly generated (Pud) lines. Rather, in terms of wing length, fecundity, egg hatch rate, and adult survival, the Wolbachia introgression conferred fitness benefits on the (Pud) lines compared to uninfected Wolbachia free wild Ae. aegypti population. wMel and wAlbB exhibited a high maternal transmission (99–100%) and induced nearly complete (98–100%) cytoplasmic incompatibility. Both the (Pud) lines were resistant to deltamethrin, malathion, DDT, and temephos, and the level of resistance was almost the same between the two lines as in the wild type. Overall, the stable association of wMel and wAlbB established with Ae. aegypti and the reproductive advantages of the (Pud) lines encourage a pilot release in the field for population replacement potential. |
| first_indexed | 2024-12-10T17:10:42Z |
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| id | doaj.art-626f3c914246454a8eb7f5aac085d56a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T17:10:42Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-626f3c914246454a8eb7f5aac085d56a2022-12-22T01:40:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.947857947857Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) linesCandasamy Sadanandane0Kasinathan Gunasekaran1Devaraju Panneer2Sarala K. Subbarao3Manju Rahi4Balakrishnan Vijayakumar5Velan Athithan6Annamalai Sakthivel7Sundaram Dinesh8Purushothaman Jambulingam9ICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaIndian Council of Medical Research, Ramalingaswami Bhawan, New Delhi, IndiaIndian Council of Medical Research, Ramalingaswami Bhawan, New Delhi, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaICMR-Vector Control Research Centre, Medical Complex, Puducherry, IndiaWolbachia, an intracellular maternally transmitted endosymbiont, has been shown to interfere with the replication of dengue virus in Aedes aegypti mosquitoes. The Wolbachia-transinfected Ae. aegypti has been currently released in many countries to test its effectiveness in preventing the transmission of dengue virus. ICMR-Vector Control Research Centre in collaboration with World Mosquito Program Monash University, Australia, has generated two new Wolbachia-introgressed Ae. aegypti Puducherry (Pud) lines via backcrossing Ae. aegypti females of Australian (Aus) strains, infected with wMel and wAlbB Wolbachia with wild-type Ae. aegypti Puducherry (Pud) males. Wolbachia infections are known to induce a fitness cost and confer benefit on the host mosquito populations that will influence spread of the Wolbachia into native wild mosquito populations during the field release. Hence, the induced fitness cost or benefit/advantage in the two newly generated Ae. aegypti (Pud) lines was assessed in the laboratory in comparison with the wild-type Ae. aegypti (Pud) strain. In addition, maternal transmission (MT) efficiency, induced cytoplasmic incompatibility (CI), and insecticide resistance status of the two (Pud) lines were determined to assess the likely frequency of wMel and wAlbB infections in the native wild population after field invasion. The study shows that wMel and wAlbB infections did not induce any fitness cost on the two newly generated (Pud) lines. Rather, in terms of wing length, fecundity, egg hatch rate, and adult survival, the Wolbachia introgression conferred fitness benefits on the (Pud) lines compared to uninfected Wolbachia free wild Ae. aegypti population. wMel and wAlbB exhibited a high maternal transmission (99–100%) and induced nearly complete (98–100%) cytoplasmic incompatibility. Both the (Pud) lines were resistant to deltamethrin, malathion, DDT, and temephos, and the level of resistance was almost the same between the two lines as in the wild type. Overall, the stable association of wMel and wAlbB established with Ae. aegypti and the reproductive advantages of the (Pud) lines encourage a pilot release in the field for population replacement potential.https://www.frontiersin.org/articles/10.3389/fmicb.2022.947857/fullWolbachiawMelwAlbBfitness costdengueAedes aegypti |
| spellingShingle | Candasamy Sadanandane Kasinathan Gunasekaran Devaraju Panneer Sarala K. Subbarao Manju Rahi Balakrishnan Vijayakumar Velan Athithan Annamalai Sakthivel Sundaram Dinesh Purushothaman Jambulingam Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines Frontiers in Microbiology Wolbachia wMel wAlbB fitness cost dengue Aedes aegypti |
| title | Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines |
| title_full | Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines |
| title_fullStr | Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines |
| title_full_unstemmed | Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines |
| title_short | Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines |
| title_sort | studies on the fitness characteristics of wmel and walbb introgressed aedes aegypti pud lines in comparison with wmel and walbb transinfected aedes aegypti aus and wild type aedes aegypti pud lines |
| topic | Wolbachia wMel wAlbB fitness cost dengue Aedes aegypti |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.947857/full |
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