Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells
Background/Aims: Berberine (BBR) is an isoquinoline alkaloid and is beneficial for the anticancer effect on a variety of human tumor cells. However, BBR's anti-angiogenesis property and its clinical potential as an inhibitor of tumor angiogenesis in breast cancer cells have not been fully eluci...
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| Format: | Article |
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Cell Physiol Biochem Press GmbH & Co KG
2013-11-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/356591 |
| _version_ | 1811335890114445312 |
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| author | Sangmin Kim Soo-Jin Oh Jeongmin Lee Jeonghun Han Myeongjin Jeon Taewoo Jung Se Kyung Lee Soo Youn Bae Jiyoung Kim Won Ho Gil Seok Won Kim Jeong Eon Lee Seok Jin Nam |
| author_facet | Sangmin Kim Soo-Jin Oh Jeongmin Lee Jeonghun Han Myeongjin Jeon Taewoo Jung Se Kyung Lee Soo Youn Bae Jiyoung Kim Won Ho Gil Seok Won Kim Jeong Eon Lee Seok Jin Nam |
| author_sort | Sangmin Kim |
| collection | DOAJ |
| description | Background/Aims: Berberine (BBR) is an isoquinoline alkaloid and is beneficial for the anticancer effect on a variety of human tumor cells. However, BBR's anti-angiogenesis property and its clinical potential as an inhibitor of tumor angiogenesis in breast cancer cells have not been fully elucidated. Here, we investigated the effect of BBR on TPA-induced VEGF and fibronectin (FN) as well as VEGF-induced FN in breast cancer cells. Methods: The secretion of VEGF protein was detected by ELISA. Fibronectin mRNA and protein expression was analyzed by Real-Time PCR and western blotting, respectively. The overexpressions of CA-MEK, and CA-Akt were examined by adenovirus system. Results: Our results showed that TPA, a tumor promoter, significantly increased the level of VEGF and FN expression in both MCF7 and T47D breast cancer cells. On the other hand, TPA-induced VEGF and FN expression was suppressed by LY294002, a PI-3K inhibitor. In contrast, the level of FN expression also significantly increased by constitutively active (CA)-AKT overexpression. We also found that TPA-induced VEGF and FN expression was decreased by BBR treatment. Finally, our results showed that VEGF augmented the expression of FN whereas VEGF-induced FN expression was decreased by BBR treatment. Conclusion: Taken together, we suggest that BBR may suppress TPA-induced VEGF and FN as well as VEGF-induced FN through the inhibition of the PI-3K/AKT pathway in breast cancer cells. Therefore, we suggest that BBR may be used as a candidate drug for the inhibition of angiogenesis of human breast cancer. |
| first_indexed | 2024-04-13T17:31:25Z |
| format | Article |
| id | doaj.art-6272598b4c684c46bd4fc72abf02c0be |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-13T17:31:25Z |
| publishDate | 2013-11-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-6272598b4c684c46bd4fc72abf02c0be2022-12-22T02:37:33ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-11-013251541155010.1159/000356591356591Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer CellsSangmin KimSoo-Jin OhJeongmin LeeJeonghun HanMyeongjin JeonTaewoo JungSe Kyung LeeSoo Youn BaeJiyoung KimWon Ho GilSeok Won KimJeong Eon LeeSeok Jin NamBackground/Aims: Berberine (BBR) is an isoquinoline alkaloid and is beneficial for the anticancer effect on a variety of human tumor cells. However, BBR's anti-angiogenesis property and its clinical potential as an inhibitor of tumor angiogenesis in breast cancer cells have not been fully elucidated. Here, we investigated the effect of BBR on TPA-induced VEGF and fibronectin (FN) as well as VEGF-induced FN in breast cancer cells. Methods: The secretion of VEGF protein was detected by ELISA. Fibronectin mRNA and protein expression was analyzed by Real-Time PCR and western blotting, respectively. The overexpressions of CA-MEK, and CA-Akt were examined by adenovirus system. Results: Our results showed that TPA, a tumor promoter, significantly increased the level of VEGF and FN expression in both MCF7 and T47D breast cancer cells. On the other hand, TPA-induced VEGF and FN expression was suppressed by LY294002, a PI-3K inhibitor. In contrast, the level of FN expression also significantly increased by constitutively active (CA)-AKT overexpression. We also found that TPA-induced VEGF and FN expression was decreased by BBR treatment. Finally, our results showed that VEGF augmented the expression of FN whereas VEGF-induced FN expression was decreased by BBR treatment. Conclusion: Taken together, we suggest that BBR may suppress TPA-induced VEGF and FN as well as VEGF-induced FN through the inhibition of the PI-3K/AKT pathway in breast cancer cells. Therefore, we suggest that BBR may be used as a candidate drug for the inhibition of angiogenesis of human breast cancer.http://www.karger.com/Article/FullText/356591BerberinePI-3KAKTVEGFFibronectin |
| spellingShingle | Sangmin Kim Soo-Jin Oh Jeongmin Lee Jeonghun Han Myeongjin Jeon Taewoo Jung Se Kyung Lee Soo Youn Bae Jiyoung Kim Won Ho Gil Seok Won Kim Jeong Eon Lee Seok Jin Nam Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells Cellular Physiology and Biochemistry Berberine PI-3K AKT VEGF Fibronectin |
| title | Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells |
| title_full | Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells |
| title_fullStr | Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells |
| title_full_unstemmed | Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells |
| title_short | Berberine Suppresses TPA-Induced Fibronectin Expression through the Inhibition of VEGF Secretion in Breast Cancer Cells |
| title_sort | berberine suppresses tpa induced fibronectin expression through the inhibition of vegf secretion in breast cancer cells |
| topic | Berberine PI-3K AKT VEGF Fibronectin |
| url | http://www.karger.com/Article/FullText/356591 |
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