Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique
The purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived from co-expressed transgenic donor cells. Cons...
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The Korean Society of Animal Reproduction and Biotechnology
2015-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=1232&vmd=Full |
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| author | Jung Seok Yang So Young Joe Bon-Chul Koo Young-Tae Heo Su Min Lee Man-Jong Kang Hyuk Song Dae Hwan Ko Sang Jun Uhm |
| author_facet | Jung Seok Yang So Young Joe Bon-Chul Koo Young-Tae Heo Su Min Lee Man-Jong Kang Hyuk Song Dae Hwan Ko Sang Jun Uhm |
| author_sort | Jung Seok Yang |
| collection | DOAJ |
| description | The purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived from co-expressed transgenic donor cells. Constructed pPiggy-mWAP-hGCSF-EF1-GFP vector was chemically transfected into bovine fetus cells and then, only GFP expressed cells were selected as donor cells for SCNT. Cleavage and blastocyst rates of parthenogenetic, SCNT embryos using non-TG cell and hGCSF-GFP dual expressed SCNT embryos were examined (cleavage rate: 78.0±2.8 vs. 73.1±3.2 vs. 70.4±4.3%, developmental rate: 27.2 ±3.2 vs. 21.9±3.1 vs. 17.0±2.9%). Result indicated that cleavage and blastocyst rates of TG embryos were significantly lower (p<0.05) than those of parthenogenetic and non-TG embryos, respectively. In this study, we successfully produced hGCSF-GFP dual expressed SCNT embryos and cryopreserved to produce transgenic cattle for bioreactor system purpose. Further process of our research will transfer of transgenic embryos to recipients and production of hGCSF secreting cattle. |
| first_indexed | 2024-12-17T00:28:57Z |
| format | Article |
| id | doaj.art-627334289b9f4875acb02c459c254f48 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-17T00:28:57Z |
| publishDate | 2015-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-627334289b9f4875acb02c459c254f482022-12-21T22:10:23ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632015-09-0130321922410.12750/JET.2015.30.3.219Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer TechniqueJung Seok Yang0So Young Joe1Bon-Chul Koo2Young-Tae Heo3Su Min Lee4Man-Jong Kang5Hyuk Song6Dae Hwan Ko7Sang Jun Uhm8Dept. of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, KoreaDept. of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, KoreaDept. of Physiology, Catholic University of Daegu School of Medicine, Daegu 38430, KoreaDept. of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, Seoul 05029, KoreaDept. of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, KoreaDept. of Animal Science, Chunnam National University, Gwangju 61186, KDept. of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, Seoul 05029, KoreaDept. of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, KoreaDept. of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, KoreaThe purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived from co-expressed transgenic donor cells. Constructed pPiggy-mWAP-hGCSF-EF1-GFP vector was chemically transfected into bovine fetus cells and then, only GFP expressed cells were selected as donor cells for SCNT. Cleavage and blastocyst rates of parthenogenetic, SCNT embryos using non-TG cell and hGCSF-GFP dual expressed SCNT embryos were examined (cleavage rate: 78.0±2.8 vs. 73.1±3.2 vs. 70.4±4.3%, developmental rate: 27.2 ±3.2 vs. 21.9±3.1 vs. 17.0±2.9%). Result indicated that cleavage and blastocyst rates of TG embryos were significantly lower (p<0.05) than those of parthenogenetic and non-TG embryos, respectively. In this study, we successfully produced hGCSF-GFP dual expressed SCNT embryos and cryopreserved to produce transgenic cattle for bioreactor system purpose. Further process of our research will transfer of transgenic embryos to recipients and production of hGCSF secreting cattle.http://www.e-jarb.org/journal/view.html?uid=1232&vmd=Fullscnthgcsfgfptransgenic embryocow |
| spellingShingle | Jung Seok Yang So Young Joe Bon-Chul Koo Young-Tae Heo Su Min Lee Man-Jong Kang Hyuk Song Dae Hwan Ko Sang Jun Uhm Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique Journal of Animal Reproduction and Biotechnology scnt hgcsf gfp transgenic embryo cow |
| title | Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique |
| title_full | Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique |
| title_fullStr | Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique |
| title_full_unstemmed | Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique |
| title_short | Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique |
| title_sort | production of hgcsf and gfp co expressed transgenic cow embryo by somatic cell nuclear transfer technique |
| topic | scnt hgcsf gfp transgenic embryo cow |
| url | http://www.e-jarb.org/journal/view.html?uid=1232&vmd=Full |
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