Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide
Abstract Background The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-021-07951-1 |
| _version_ | 1819118122839310336 |
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| author | David A. Skerrett-Byrne Brett Nixon Elizabeth G. Bromfield James Breen Natalie A. Trigg Simone J. Stanger Ilana R. Bernstein Amanda L. Anderson Tessa Lord R. John Aitken Shaun D. Roman Sarah A. Robertson John E. Schjenken |
| author_facet | David A. Skerrett-Byrne Brett Nixon Elizabeth G. Bromfield James Breen Natalie A. Trigg Simone J. Stanger Ilana R. Bernstein Amanda L. Anderson Tessa Lord R. John Aitken Shaun D. Roman Sarah A. Robertson John E. Schjenken |
| author_sort | David A. Skerrett-Byrne |
| collection | DOAJ |
| description | Abstract Background The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection. Results A total of 15,304 genes were identified in the seminal vesicles with those encoding secreted proteins amongst the most abundant. In addition to reproductive hormone pathways, functional annotation of the seminal vesicle transcriptome identified cell proliferation, protein synthesis, and cellular death and survival pathways as prominent biological processes. Administration of acrylamide elicited 70 differentially regulated (fold-change ≥1.5 or ≤ 0.67) genes, several of which were orthogonally validated using quantitative PCR. Pathways that initiate gene and protein synthesis to promote cellular survival were prominent amongst the dysregulated pathways. Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. Early growth response (Egr1), C-C motif chemokine ligand 8 (Ccl8), and Collagen, type V, alpha 1 (Col5a1) were also identified amongst the dysregulated genes. Additionally, acrylamide treatment led to subtle changes in the expression of genes that encode proteins secreted by the seminal vesicle, including the complement regulator, Complement factor b (Cfb). Conclusions These data add to emerging evidence demonstrating that the seminal vesicles, like other male reproductive tract tissues, are sensitive to environmental insults, and respond in a manner with potential to exert impact on fetal development and later offspring health. |
| first_indexed | 2024-12-22T05:43:52Z |
| format | Article |
| id | doaj.art-627867058628482e8338e8767450f0aa |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T05:43:52Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-627867058628482e8338e8767450f0aa2022-12-21T18:37:06ZengBMCBMC Genomics1471-21642021-10-0122112210.1186/s12864-021-07951-1Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamideDavid A. Skerrett-Byrne0Brett Nixon1Elizabeth G. Bromfield2James Breen3Natalie A. Trigg4Simone J. Stanger5Ilana R. Bernstein6Amanda L. Anderson7Tessa Lord8R. John Aitken9Shaun D. Roman10Sarah A. Robertson11John E. Schjenken12Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastleThe Robinson Research Institute and Adelaide Medical School, University of AdelaidePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastlePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastleThe Robinson Research Institute and Adelaide Medical School, University of AdelaidePriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of NewcastleAbstract Background The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection. Results A total of 15,304 genes were identified in the seminal vesicles with those encoding secreted proteins amongst the most abundant. In addition to reproductive hormone pathways, functional annotation of the seminal vesicle transcriptome identified cell proliferation, protein synthesis, and cellular death and survival pathways as prominent biological processes. Administration of acrylamide elicited 70 differentially regulated (fold-change ≥1.5 or ≤ 0.67) genes, several of which were orthogonally validated using quantitative PCR. Pathways that initiate gene and protein synthesis to promote cellular survival were prominent amongst the dysregulated pathways. Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. Early growth response (Egr1), C-C motif chemokine ligand 8 (Ccl8), and Collagen, type V, alpha 1 (Col5a1) were also identified amongst the dysregulated genes. Additionally, acrylamide treatment led to subtle changes in the expression of genes that encode proteins secreted by the seminal vesicle, including the complement regulator, Complement factor b (Cfb). Conclusions These data add to emerging evidence demonstrating that the seminal vesicles, like other male reproductive tract tissues, are sensitive to environmental insults, and respond in a manner with potential to exert impact on fetal development and later offspring health.https://doi.org/10.1186/s12864-021-07951-1AcrylamideReproductionReproductive toxicantSeminal vesicleTranscriptomics |
| spellingShingle | David A. Skerrett-Byrne Brett Nixon Elizabeth G. Bromfield James Breen Natalie A. Trigg Simone J. Stanger Ilana R. Bernstein Amanda L. Anderson Tessa Lord R. John Aitken Shaun D. Roman Sarah A. Robertson John E. Schjenken Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide BMC Genomics Acrylamide Reproduction Reproductive toxicant Seminal vesicle Transcriptomics |
| title | Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide |
| title_full | Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide |
| title_fullStr | Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide |
| title_full_unstemmed | Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide |
| title_short | Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide |
| title_sort | transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide |
| topic | Acrylamide Reproduction Reproductive toxicant Seminal vesicle Transcriptomics |
| url | https://doi.org/10.1186/s12864-021-07951-1 |
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