Subcellular Distribution and Genotoxicity of Silica Nanoparticles in Human Bronchial Epithelial Cells
Background and objective Silicon nanoparticles are widely used in daily life. Therefore, they attract increased attention because of their potential biotoxicity to the lungs when inhaled. The aims of this study are to explore the organism distribution and genotoxicity of silica nanoparticles in huma...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2013-03-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2013.03.01 |
| _version_ | 1818862582328459264 |
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| author | Guangqiang ZHAO Yunchao HUANG Guangjian LI Sen LI Yongchun ZHOU Yujie LEI Xiaobo CHEN Kaiyun YANG Ying CHEN Kun YANG |
| author_facet | Guangqiang ZHAO Yunchao HUANG Guangjian LI Sen LI Yongchun ZHOU Yujie LEI Xiaobo CHEN Kaiyun YANG Ying CHEN Kun YANG |
| author_sort | Guangqiang ZHAO |
| collection | DOAJ |
| description | Background and objective Silicon nanoparticles are widely used in daily life. Therefore, they attract increased attention because of their potential biotoxicity to the lungs when inhaled. The aims of this study are to explore the organism distribution and genotoxicity of silica nanoparticles in human bronchial epithelial cells (BEAS-2B). Methods The biodistribution of silica with different particle sizes in human bronchial epithelial cells was observed by transmission electron microscopy (TEM). DNA damage was detected by single-cell gel electrophoresis (comet assay). Results TEM revealed that SiO2 nanoparticles with different sizes can be uptaken by cells and be localized in the cytoplasm and the nucleus. Compared with micro-silica, nano-silica in BEAS-2B cells can inflict more severe DNA damage (P<0.05). Conclusion The particle size of silica nanoparticles can be used to determine their distribution in biological cells. Compared with micro-silica, nano-silica has higher genotoxicity. |
| first_indexed | 2024-12-19T10:02:09Z |
| format | Article |
| id | doaj.art-6278ae1882904099be3573d600e9ad5c |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-19T10:02:09Z |
| publishDate | 2013-03-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-6278ae1882904099be3573d600e9ad5c2022-12-21T20:26:36ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872013-03-0116311712410.3779/j.issn.1009-3419.2013.03.01Subcellular Distribution and Genotoxicity of Silica Nanoparticles
in Human Bronchial Epithelial CellsGuangqiang ZHAOYunchao HUANGGuangjian LISen LIYongchun ZHOUYujie LEIXiaobo CHENKaiyun YANGYing CHENKun YANGBackground and objective Silicon nanoparticles are widely used in daily life. Therefore, they attract increased attention because of their potential biotoxicity to the lungs when inhaled. The aims of this study are to explore the organism distribution and genotoxicity of silica nanoparticles in human bronchial epithelial cells (BEAS-2B). Methods The biodistribution of silica with different particle sizes in human bronchial epithelial cells was observed by transmission electron microscopy (TEM). DNA damage was detected by single-cell gel electrophoresis (comet assay). Results TEM revealed that SiO2 nanoparticles with different sizes can be uptaken by cells and be localized in the cytoplasm and the nucleus. Compared with micro-silica, nano-silica in BEAS-2B cells can inflict more severe DNA damage (P<0.05). Conclusion The particle size of silica nanoparticles can be used to determine their distribution in biological cells. Compared with micro-silica, nano-silica has higher genotoxicity.http://dx.doi.org/10.3779/j.issn.1009-3419.2013.03.01Silica nanoparticlesHuman bronchial epithelial cellsBiological distributionGenotoxicity |
| spellingShingle | Guangqiang ZHAO Yunchao HUANG Guangjian LI Sen LI Yongchun ZHOU Yujie LEI Xiaobo CHEN Kaiyun YANG Ying CHEN Kun YANG Subcellular Distribution and Genotoxicity of Silica Nanoparticles in Human Bronchial Epithelial Cells Chinese Journal of Lung Cancer Silica nanoparticles Human bronchial epithelial cells Biological distribution Genotoxicity |
| title | Subcellular Distribution and Genotoxicity of Silica Nanoparticles
in Human Bronchial Epithelial Cells |
| title_full | Subcellular Distribution and Genotoxicity of Silica Nanoparticles
in Human Bronchial Epithelial Cells |
| title_fullStr | Subcellular Distribution and Genotoxicity of Silica Nanoparticles
in Human Bronchial Epithelial Cells |
| title_full_unstemmed | Subcellular Distribution and Genotoxicity of Silica Nanoparticles
in Human Bronchial Epithelial Cells |
| title_short | Subcellular Distribution and Genotoxicity of Silica Nanoparticles
in Human Bronchial Epithelial Cells |
| title_sort | subcellular distribution and genotoxicity of silica nanoparticles
in human bronchial epithelial cells |
| topic | Silica nanoparticles Human bronchial epithelial cells Biological distribution Genotoxicity |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2013.03.01 |
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