Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens
【Objective】A disease model of fowl adenovirus serotype 2 (FAdV-2) was established to provide a refference for evaluating the immunogenicity and vaccine effectiveness of FAdV-2.【Method】The samples suspected to be infected with avian adenovirus were isolated and identified. The genetic evolution of th...
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| Format: | Article |
| Language: | English |
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Guangdong Academy of Agricultural Sciences
2023-07-01
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| Series: | Guangdong nongye kexue |
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| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202307015 |
| _version_ | 1797676680781758464 |
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| author | Xinyu HU Minhua SUN Qinghang ZENG Zimin XIE Zhaoxia YUAN Ming LIAO |
| author_facet | Xinyu HU Minhua SUN Qinghang ZENG Zimin XIE Zhaoxia YUAN Ming LIAO |
| author_sort | Xinyu HU |
| collection | DOAJ |
| description | 【Objective】A disease model of fowl adenovirus serotype 2 (FAdV-2) was established to provide a refference for evaluating the immunogenicity and vaccine effectiveness of FAdV-2.【Method】The samples suspected to be infected with avian adenovirus were isolated and identified. The genetic evolution of the Fiber and Hexon gene sequences of the isolates was analyzed. The nucleotide and amino acid sequences of the isolated strain and other FAdV representative strains were analyzed using DNAStar and MEGA7.0. After determining that the isolated virus was FAdV-2, the pathogenicity of the isolated strain was analyzed in 6-week-old SPF chickens under different infection routes and different infection doses in animal experiment. The best infection model and infection dose were selected to establish the FAdV-2 pathogenesis model.【Result】One FAdV-2 strain was successfully isolated from the infected chicken tissue samples, named KM strain. Genetic evolution analysis shows that KM strain and GX01 strain belong to the FAdV-2 of avian adenovirus species D of group Ⅰ. The homology analysis shows that the nucleotide similarity of Fiber gene and Hexon gene between KM strain and GX01 strain is 98.9% and 99.3%, and the amino acid similarity is 98.9% and 98.8%, respectively. The pathogenicity of intravenous injection was higher than that of intramuscular injection, and the chickens showed obvious hepatitis hydropericardium syndrome (HHS). The infection route of the KM strain model was intravenous injection, and the infection dose was 108.0TCID50. The chickens died after infection, with a mortality rate of 50%. Obvious pathological changes were found in the dead chickens at necropsy, and the chickens were positive for cloaca virus shedding at 1 d post infection. The established KM strain disease model provides a certain reference for vaccine development and vaccine evaluation, which will effectively prevent and reduce the transmission of FAdV-2.【Conclusion】It is verified that FAdV-2 can cause hepatitis hydropericardium syndrome, and a pathogenesis model of FAdV-2 infection in SPF chickens is established for the first time. |
| first_indexed | 2024-03-11T22:33:47Z |
| format | Article |
| id | doaj.art-627a308a540e4a73ac5c55eee4ee18db |
| institution | Directory Open Access Journal |
| issn | 1004-874X |
| language | English |
| last_indexed | 2024-03-11T22:33:47Z |
| publishDate | 2023-07-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
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| series | Guangdong nongye kexue |
| spelling | doaj.art-627a308a540e4a73ac5c55eee4ee18db2023-09-23T02:35:22ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2023-07-0150714615510.16768/j.issn.1004-874X.2023.07.015202307015Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF ChickensXinyu HU0Minhua SUN1Qinghang ZENG2Zimin XIE3Zhaoxia YUAN4Ming LIAO5College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences / Guangdong Key Laboratory of Livestock Disease Prevention / Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences / Guangdong Key Laboratory of Livestock Disease Prevention / Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences / Guangdong Key Laboratory of Livestock Disease Prevention / Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China【Objective】A disease model of fowl adenovirus serotype 2 (FAdV-2) was established to provide a refference for evaluating the immunogenicity and vaccine effectiveness of FAdV-2.【Method】The samples suspected to be infected with avian adenovirus were isolated and identified. The genetic evolution of the Fiber and Hexon gene sequences of the isolates was analyzed. The nucleotide and amino acid sequences of the isolated strain and other FAdV representative strains were analyzed using DNAStar and MEGA7.0. After determining that the isolated virus was FAdV-2, the pathogenicity of the isolated strain was analyzed in 6-week-old SPF chickens under different infection routes and different infection doses in animal experiment. The best infection model and infection dose were selected to establish the FAdV-2 pathogenesis model.【Result】One FAdV-2 strain was successfully isolated from the infected chicken tissue samples, named KM strain. Genetic evolution analysis shows that KM strain and GX01 strain belong to the FAdV-2 of avian adenovirus species D of group Ⅰ. The homology analysis shows that the nucleotide similarity of Fiber gene and Hexon gene between KM strain and GX01 strain is 98.9% and 99.3%, and the amino acid similarity is 98.9% and 98.8%, respectively. The pathogenicity of intravenous injection was higher than that of intramuscular injection, and the chickens showed obvious hepatitis hydropericardium syndrome (HHS). The infection route of the KM strain model was intravenous injection, and the infection dose was 108.0TCID50. The chickens died after infection, with a mortality rate of 50%. Obvious pathological changes were found in the dead chickens at necropsy, and the chickens were positive for cloaca virus shedding at 1 d post infection. The established KM strain disease model provides a certain reference for vaccine development and vaccine evaluation, which will effectively prevent and reduce the transmission of FAdV-2.【Conclusion】It is verified that FAdV-2 can cause hepatitis hydropericardium syndrome, and a pathogenesis model of FAdV-2 infection in SPF chickens is established for the first time.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202307015avian adenovirusanimal modelisolation and identificationfowl adenovirus serotype 2 (fadv-2)pathogenicityhydropericardium hepatitis syndrome |
| spellingShingle | Xinyu HU Minhua SUN Qinghang ZENG Zimin XIE Zhaoxia YUAN Ming LIAO Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens Guangdong nongye kexue avian adenovirus animal model isolation and identification fowl adenovirus serotype 2 (fadv-2) pathogenicity hydropericardium hepatitis syndrome |
| title | Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens |
| title_full | Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens |
| title_fullStr | Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens |
| title_full_unstemmed | Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens |
| title_short | Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens |
| title_sort | isolation and identification of fowl adenovirus serotype 2 and establishment of a disease model for spf chickens |
| topic | avian adenovirus animal model isolation and identification fowl adenovirus serotype 2 (fadv-2) pathogenicity hydropericardium hepatitis syndrome |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202307015 |
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