UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity
The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H<sub>2</sub>O<sub>2</sub>-degraded polysaccharides from <i>Sargassum fusiforme</i>. Results indicated that twofraction...
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MDPI AG
2023-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/11/561 |
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| author | Shiyuan Chang Xiaoyong Chen Yifan Chen Lijun You Kseniya Hileuskaya |
| author_facet | Shiyuan Chang Xiaoyong Chen Yifan Chen Lijun You Kseniya Hileuskaya |
| author_sort | Shiyuan Chang |
| collection | DOAJ |
| description | The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H<sub>2</sub>O<sub>2</sub>-degraded polysaccharides from <i>Sargassum fusiforme</i>. Results indicated that twofractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fuc<i>p</i>, t-Man<i>p</i>, 6-Gal<i>p</i>, t-Fuc<i>p</i>, and 3,4-GlcA<i>p</i>. These results would provide theoretical support for studying the structural properties and biological activities of UV/H<sub>2</sub>O<sub>2</sub>-degraded polysaccharides. |
| first_indexed | 2024-03-09T16:39:06Z |
| format | Article |
| id | doaj.art-627f96bf04954ee984784825fa1f84d7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T16:39:06Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj.art-627f96bf04954ee984784825fa1f84d72023-11-24T14:53:08ZengMDPI AGMarine Drugs1660-33972023-10-01211156110.3390/md21110561UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory ActivityShiyuan Chang0Xiaoyong Chen1Yifan Chen2Lijun You3Kseniya Hileuskaya4School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaInstitute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36F. Skaryna Str., 220141 Minsk, BelarusThe main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H<sub>2</sub>O<sub>2</sub>-degraded polysaccharides from <i>Sargassum fusiforme</i>. Results indicated that twofractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fuc<i>p</i>, t-Man<i>p</i>, 6-Gal<i>p</i>, t-Fuc<i>p</i>, and 3,4-GlcA<i>p</i>. These results would provide theoretical support for studying the structural properties and biological activities of UV/H<sub>2</sub>O<sub>2</sub>-degraded polysaccharides.https://www.mdpi.com/1660-3397/21/11/561polysaccharides<i>Sargassum fusiforme</i>degradationUV/H<sub>2</sub>O<sub>2</sub>anti-inflammatory activity |
| spellingShingle | Shiyuan Chang Xiaoyong Chen Yifan Chen Lijun You Kseniya Hileuskaya UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity Marine Drugs polysaccharides <i>Sargassum fusiforme</i> degradation UV/H<sub>2</sub>O<sub>2</sub> anti-inflammatory activity |
| title | UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity |
| title_full | UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity |
| title_fullStr | UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity |
| title_full_unstemmed | UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity |
| title_short | UV/H<sub>2</sub>O<sub>2</sub>-Degraded Polysaccharides from <i>Sargassum fusiforme</i>: Purification, Structural Properties, and Anti-Inflammatory Activity |
| title_sort | uv h sub 2 sub o sub 2 sub degraded polysaccharides from i sargassum fusiforme i purification structural properties and anti inflammatory activity |
| topic | polysaccharides <i>Sargassum fusiforme</i> degradation UV/H<sub>2</sub>O<sub>2</sub> anti-inflammatory activity |
| url | https://www.mdpi.com/1660-3397/21/11/561 |
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