Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA.
Curcumin attracts worldwide scientific interest due to its anti-proliferative and apoptosis inducing effects on different tumor cells at concentrations ranging from 10 to 150 µM (3.7-55 µg/ml). Unfortunately, because of a low oral bioavailability, only low and pharmacologically ineffective serum lev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3821844?pdf=render |
| _version_ | 1819053944720064512 |
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| author | Stephan Buss Jadranka Dobra Kerstin Goerg Stephanie Hoffmann Stefan Kippenberger Roland Kaufmann Matthias Hofmann August Bernd |
| author_facet | Stephan Buss Jadranka Dobra Kerstin Goerg Stephanie Hoffmann Stefan Kippenberger Roland Kaufmann Matthias Hofmann August Bernd |
| author_sort | Stephan Buss |
| collection | DOAJ |
| description | Curcumin attracts worldwide scientific interest due to its anti-proliferative and apoptosis inducing effects on different tumor cells at concentrations ranging from 10 to 150 µM (3.7-55 µg/ml). Unfortunately, because of a low oral bioavailability, only low and pharmacologically ineffective serum levels are achievable. In this study, an alternative treatment concept consisting of low concentration curcumin (0.2-5 µg/ml) and irradiation with UVA or visible light (VL) has been tested. The experimental results show clearly that this treatment decreases the proliferation and the viability of human melanoma cells while the cell membrane integrity remains intact. We identified the onset of apoptosis characterized by typical markers such as active caspases 8, 9 and 3 as well as DNA fragmentation accompanied by the loss of cell adhesion. The mitochondrial apoptosis signaling pathway is predominant due to an early activation of caspase-9. The present data indicate a higher efficacy of a combination of curcumin and VL than curcumin and UVA. Reduced effects as a result of light absorption by heavily pigmented skin are unlikely if VL is used. These results indicate that a combination of curcumin and light irradiation may be a useful additional therapy in the treatment of malignant disease. |
| first_indexed | 2024-12-21T12:43:47Z |
| format | Article |
| id | doaj.art-62842c69049f467594cc68c6f10abf57 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T12:43:47Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-62842c69049f467594cc68c6f10abf572022-12-21T19:03:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7974810.1371/journal.pone.0079748Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA.Stephan BussJadranka DobraKerstin GoergStephanie HoffmannStefan KippenbergerRoland KaufmannMatthias HofmannAugust BerndCurcumin attracts worldwide scientific interest due to its anti-proliferative and apoptosis inducing effects on different tumor cells at concentrations ranging from 10 to 150 µM (3.7-55 µg/ml). Unfortunately, because of a low oral bioavailability, only low and pharmacologically ineffective serum levels are achievable. In this study, an alternative treatment concept consisting of low concentration curcumin (0.2-5 µg/ml) and irradiation with UVA or visible light (VL) has been tested. The experimental results show clearly that this treatment decreases the proliferation and the viability of human melanoma cells while the cell membrane integrity remains intact. We identified the onset of apoptosis characterized by typical markers such as active caspases 8, 9 and 3 as well as DNA fragmentation accompanied by the loss of cell adhesion. The mitochondrial apoptosis signaling pathway is predominant due to an early activation of caspase-9. The present data indicate a higher efficacy of a combination of curcumin and VL than curcumin and UVA. Reduced effects as a result of light absorption by heavily pigmented skin are unlikely if VL is used. These results indicate that a combination of curcumin and light irradiation may be a useful additional therapy in the treatment of malignant disease.http://europepmc.org/articles/PMC3821844?pdf=render |
| spellingShingle | Stephan Buss Jadranka Dobra Kerstin Goerg Stephanie Hoffmann Stefan Kippenberger Roland Kaufmann Matthias Hofmann August Bernd Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. PLoS ONE |
| title | Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. |
| title_full | Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. |
| title_fullStr | Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. |
| title_full_unstemmed | Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. |
| title_short | Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. |
| title_sort | visible light is a better co inducer of apoptosis for curcumin treated human melanoma cells than uva |
| url | http://europepmc.org/articles/PMC3821844?pdf=render |
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