Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity
Skeletal muscles are formed in numerous shapes and sizes, and this diversity impacts function and disease susceptibility. To understand how muscle diversity is generated, we performed gene expression profiling of two muscle subsets from Drosophila embryos. By comparing the transcriptional profiles o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-08-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714005725 |
| _version_ | 1818034029856817152 |
|---|---|
| author | Krista C. Dobi Marc S. Halfon Mary K. Baylies |
| author_facet | Krista C. Dobi Marc S. Halfon Mary K. Baylies |
| author_sort | Krista C. Dobi |
| collection | DOAJ |
| description | Skeletal muscles are formed in numerous shapes and sizes, and this diversity impacts function and disease susceptibility. To understand how muscle diversity is generated, we performed gene expression profiling of two muscle subsets from Drosophila embryos. By comparing the transcriptional profiles of these subsets, we identified a core group of founder cell-enriched genes. We screened mutants for muscle defects and identified functions for Sin3A and 10 other transcription and chromatin regulators in the Drosophila embryonic somatic musculature. Sin3A is required for the morphogenesis of a muscle subset, and Sin3A mutants display muscle loss and misattachment. Additionally, misexpression of identity gene transcription factors in Sin3A heterozygous embryos leads to direct transformations of one muscle into another, whereas overexpression of Sin3A results in the reverse transformation. Our data implicate Sin3A as a key buffer controlling muscle responsiveness to transcription factors in the formation of muscle identity, thereby generating tissue diversity. |
| first_indexed | 2024-12-10T06:32:40Z |
| format | Article |
| id | doaj.art-628522a336b8480bb9d1d5e168e33a64 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-10T06:32:40Z |
| publishDate | 2014-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-628522a336b8480bb9d1d5e168e33a642022-12-22T01:59:02ZengElsevierCell Reports2211-12472014-08-018385887010.1016/j.celrep.2014.07.005Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle IdentityKrista C. Dobi0Marc S. Halfon1Mary K. Baylies2Program in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USADepartment of Biochemistry, State University of New York at Buffalo, Buffalo, NY 14203, USAProgram in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USASkeletal muscles are formed in numerous shapes and sizes, and this diversity impacts function and disease susceptibility. To understand how muscle diversity is generated, we performed gene expression profiling of two muscle subsets from Drosophila embryos. By comparing the transcriptional profiles of these subsets, we identified a core group of founder cell-enriched genes. We screened mutants for muscle defects and identified functions for Sin3A and 10 other transcription and chromatin regulators in the Drosophila embryonic somatic musculature. Sin3A is required for the morphogenesis of a muscle subset, and Sin3A mutants display muscle loss and misattachment. Additionally, misexpression of identity gene transcription factors in Sin3A heterozygous embryos leads to direct transformations of one muscle into another, whereas overexpression of Sin3A results in the reverse transformation. Our data implicate Sin3A as a key buffer controlling muscle responsiveness to transcription factors in the formation of muscle identity, thereby generating tissue diversity.http://www.sciencedirect.com/science/article/pii/S2211124714005725 |
| spellingShingle | Krista C. Dobi Marc S. Halfon Mary K. Baylies Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity Cell Reports |
| title | Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity |
| title_full | Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity |
| title_fullStr | Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity |
| title_full_unstemmed | Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity |
| title_short | Whole-Genome Analysis of Muscle Founder Cells Implicates the Chromatin Regulator Sin3A in Muscle Identity |
| title_sort | whole genome analysis of muscle founder cells implicates the chromatin regulator sin3a in muscle identity |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714005725 |
| work_keys_str_mv | AT kristacdobi wholegenomeanalysisofmusclefoundercellsimplicatesthechromatinregulatorsin3ainmuscleidentity AT marcshalfon wholegenomeanalysisofmusclefoundercellsimplicatesthechromatinregulatorsin3ainmuscleidentity AT marykbaylies wholegenomeanalysisofmusclefoundercellsimplicatesthechromatinregulatorsin3ainmuscleidentity |