Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds
The improvement of the reproductive traits of animals is of great interest for livestock production. Due to its positive impact on the sheep industry’s profitability, prolificacy is one of the most economically significant biological traits, showing variation between and within breeds of domestic sh...
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MDPI AG
2023-04-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/13/5/996 |
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| author | Alexandru Marius Deac Adriana Sebastiana Musca Stefania Dana Mesesan Marius Gavril Aipatioaie Adrian Ionascu Viorica Cosier Attila Cristian Ratiu Ileana Miclea Ioan Ladosi Marius Zahan |
| author_facet | Alexandru Marius Deac Adriana Sebastiana Musca Stefania Dana Mesesan Marius Gavril Aipatioaie Adrian Ionascu Viorica Cosier Attila Cristian Ratiu Ileana Miclea Ioan Ladosi Marius Zahan |
| author_sort | Alexandru Marius Deac |
| collection | DOAJ |
| description | The improvement of the reproductive traits of animals is of great interest for livestock production. Due to its positive impact on the sheep industry’s profitability, prolificacy is one of the most economically significant biological traits, showing variation between and within breeds of domestic sheep (<i>Ovis aries</i>). Different mutations in<i> BMPR-1B</i>, <i>BMP15</i> and <i>GDF9</i> genes coding for the transforming growth factor-β (TGFβ) superfamily have been shown to influence the ovulation rate and litter size. Numerous single-nucleotide polymorphisms (SNPs) in the <i>bone morphogenetic protein 15</i> (<i>BMP15</i>) gene have been linked to ewe fecundity. Using targeted PCR amplification and Sanger sequencing, we were able to identify heterozygous SNPs in exon 2 of <i>BMP15</i> in three sheep breeds reared in Romania: Tsigai, Cluj Merino and Tsurcana. The sequence analysis revealed three previously documented mutations, namely the missense mutation c.755T>C (L252P), which is predicted to change the tertiary structure of the <i>BMP15</i> protein, and two silent mutations, c.747T>C (P249P) and c.1047G>A (V349V). In addition, we also identified one novel silent mutation, c.825G>A (S275S). Based on our findings and publicly available data, we indicate four putative mutational hotspots within exon 2 of <i>BMP15</i> that could be considered for improving the indigenous sheep breeds through targeted gene editing and SNP genotyping strategies. |
| first_indexed | 2024-03-11T04:02:39Z |
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| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-11T04:02:39Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-6285fdf40cb74034930cdef6133a8ba22023-11-18T00:02:24ZengMDPI AGAgriculture2077-04722023-04-0113599610.3390/agriculture13050996Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep BreedsAlexandru Marius Deac0Adriana Sebastiana Musca1Stefania Dana Mesesan2Marius Gavril Aipatioaie3Adrian Ionascu4Viorica Cosier5Attila Cristian Ratiu6Ileana Miclea7Ioan Ladosi8Marius Zahan9Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaDepartment of Genetics, Faculty of Biology, University of Bucharest, 060101 Bucharest, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaDepartment of Genetics, Faculty of Biology, University of Bucharest, 060101 Bucharest, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, RomaniaThe improvement of the reproductive traits of animals is of great interest for livestock production. Due to its positive impact on the sheep industry’s profitability, prolificacy is one of the most economically significant biological traits, showing variation between and within breeds of domestic sheep (<i>Ovis aries</i>). Different mutations in<i> BMPR-1B</i>, <i>BMP15</i> and <i>GDF9</i> genes coding for the transforming growth factor-β (TGFβ) superfamily have been shown to influence the ovulation rate and litter size. Numerous single-nucleotide polymorphisms (SNPs) in the <i>bone morphogenetic protein 15</i> (<i>BMP15</i>) gene have been linked to ewe fecundity. Using targeted PCR amplification and Sanger sequencing, we were able to identify heterozygous SNPs in exon 2 of <i>BMP15</i> in three sheep breeds reared in Romania: Tsigai, Cluj Merino and Tsurcana. The sequence analysis revealed three previously documented mutations, namely the missense mutation c.755T>C (L252P), which is predicted to change the tertiary structure of the <i>BMP15</i> protein, and two silent mutations, c.747T>C (P249P) and c.1047G>A (V349V). In addition, we also identified one novel silent mutation, c.825G>A (S275S). Based on our findings and publicly available data, we indicate four putative mutational hotspots within exon 2 of <i>BMP15</i> that could be considered for improving the indigenous sheep breeds through targeted gene editing and SNP genotyping strategies.https://www.mdpi.com/2077-0472/13/5/996SNPsBMP15FecXTsigaiCluj MerinoTsurcana |
| spellingShingle | Alexandru Marius Deac Adriana Sebastiana Musca Stefania Dana Mesesan Marius Gavril Aipatioaie Adrian Ionascu Viorica Cosier Attila Cristian Ratiu Ileana Miclea Ioan Ladosi Marius Zahan Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds Agriculture SNPs BMP15 FecX Tsigai Cluj Merino Tsurcana |
| title | Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds |
| title_full | Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds |
| title_fullStr | Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds |
| title_full_unstemmed | Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds |
| title_short | Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds |
| title_sort | single nucleotide polymorphisms identified within exon 2 of fertility associated bone morphogenetic protein bmp15 gene in three romanian sheep breeds |
| topic | SNPs BMP15 FecX Tsigai Cluj Merino Tsurcana |
| url | https://www.mdpi.com/2077-0472/13/5/996 |
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