Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages
Scavenger receptor (SR)-BI mediates the selective uptake of high density lipoprotein (HDL) cholesteryl esters and the efflux of free cholesterol. In Chinese hamster ovary (CHO) cells, SR-BI is predominantly associated with caveolae which we have recently demonstrated are the initial loci for membran...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1999-09-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520334106 |
| _version_ | 1818671411247448064 |
|---|---|
| author | Sergey Matveev Deneys R. van der Westhuyzen Eric J. Smart |
| author_facet | Sergey Matveev Deneys R. van der Westhuyzen Eric J. Smart |
| author_sort | Sergey Matveev |
| collection | DOAJ |
| description | Scavenger receptor (SR)-BI mediates the selective uptake of high density lipoprotein (HDL) cholesteryl esters and the efflux of free cholesterol. In Chinese hamster ovary (CHO) cells, SR-BI is predominantly associated with caveolae which we have recently demonstrated are the initial loci for membrane transfer of HDL cholesteryl esters. Because cholesterol accumulation in macrophages is a critical event in atherogenesis, we investigated the expression of SR-BI and caveolin-1 in several macrophage cell lines. Human THP-1 monocytes were examined before and after differentiation to macrophages by treatment with 200 nm phorbol ester for 72 h. Undifferentiated THP-1 cells expressed caveolin-1 weakly whereas differentiation up-regulated caveolin-1 expression greater than 50-fold. In contrast, both undifferentiated and differentiated THP-1 cells expressed similar levels of SR-BI. Differentiation of THP-1 cells increased the percent of membrane cholesterol associated with caveolae from 12% ± 1.9% to 38% ± 3.1%. The increase in caveolin-1 expression was associated with a 2- to 3-fold increase in selective cholesterol ether uptake from HDL. Two mouse macrophage cell lines, J774 and RAW, expressed levels of SR-BI similar to differentiated THP-1 cells but did not express detectable levels of caveolin-1. In comparison to differentiated THP-1 cells, RAW and J774 cells internalized 9- to 10-fold less cholesteryl ester. We conclude that differentiated THP-1 cells express both caveolin-1 and SR-BI and that their co-expression is associated with enhanced selective cholesteryl ester uptake.— Matveev, S., D. R. van der Westhuyzen, and E. J. Smart. Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages. J. Lipid Res. 1999. 40: 1647–1654. |
| first_indexed | 2024-12-17T07:23:34Z |
| format | Article |
| id | doaj.art-6289a772cc5543579eb1e95fcbf4122f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T07:23:34Z |
| publishDate | 1999-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-6289a772cc5543579eb1e95fcbf4122f2022-12-21T21:58:41ZengElsevierJournal of Lipid Research0022-22751999-09-0140916471654Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophagesSergey Matveev0Deneys R. van der Westhuyzen1Eric J. Smart2Departments of Physiology, University of Kentucky Medical School, 800 Rose Street, Lexington, KY 40536Internal Medicine, University of Kentucky Medical School, 800 Rose Street, Lexington, KY 40536To whom correspondence should be addressed.; Departments of Physiology, University of Kentucky Medical School, 800 Rose Street, Lexington, KY 40536Scavenger receptor (SR)-BI mediates the selective uptake of high density lipoprotein (HDL) cholesteryl esters and the efflux of free cholesterol. In Chinese hamster ovary (CHO) cells, SR-BI is predominantly associated with caveolae which we have recently demonstrated are the initial loci for membrane transfer of HDL cholesteryl esters. Because cholesterol accumulation in macrophages is a critical event in atherogenesis, we investigated the expression of SR-BI and caveolin-1 in several macrophage cell lines. Human THP-1 monocytes were examined before and after differentiation to macrophages by treatment with 200 nm phorbol ester for 72 h. Undifferentiated THP-1 cells expressed caveolin-1 weakly whereas differentiation up-regulated caveolin-1 expression greater than 50-fold. In contrast, both undifferentiated and differentiated THP-1 cells expressed similar levels of SR-BI. Differentiation of THP-1 cells increased the percent of membrane cholesterol associated with caveolae from 12% ± 1.9% to 38% ± 3.1%. The increase in caveolin-1 expression was associated with a 2- to 3-fold increase in selective cholesterol ether uptake from HDL. Two mouse macrophage cell lines, J774 and RAW, expressed levels of SR-BI similar to differentiated THP-1 cells but did not express detectable levels of caveolin-1. In comparison to differentiated THP-1 cells, RAW and J774 cells internalized 9- to 10-fold less cholesteryl ester. We conclude that differentiated THP-1 cells express both caveolin-1 and SR-BI and that their co-expression is associated with enhanced selective cholesteryl ester uptake.— Matveev, S., D. R. van der Westhuyzen, and E. J. Smart. Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages. J. Lipid Res. 1999. 40: 1647–1654.http://www.sciencedirect.com/science/article/pii/S0022227520334106caveolaeSR-BICD36HDLfoam cells |
| spellingShingle | Sergey Matveev Deneys R. van der Westhuyzen Eric J. Smart Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages Journal of Lipid Research caveolae SR-BI CD36 HDL foam cells |
| title | Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages |
| title_full | Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages |
| title_fullStr | Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages |
| title_full_unstemmed | Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages |
| title_short | Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages |
| title_sort | co expression of scavenger receptor bi and caveolin 1 is associated with enhanced selective cholesteryl ester uptake in thp 1 macrophages |
| topic | caveolae SR-BI CD36 HDL foam cells |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520334106 |
| work_keys_str_mv | AT sergeymatveev coexpressionofscavengerreceptorbiandcaveolin1isassociatedwithenhancedselectivecholesterylesteruptakeinthp1macrophages AT deneysrvanderwesthuyzen coexpressionofscavengerreceptorbiandcaveolin1isassociatedwithenhancedselectivecholesterylesteruptakeinthp1macrophages AT ericjsmart coexpressionofscavengerreceptorbiandcaveolin1isassociatedwithenhancedselectivecholesterylesteruptakeinthp1macrophages |