Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus
Viruses require energy and biosynthetic precursors from host cells for replication. An understanding of the metabolic interplay between classical swine fever virus (CSFV) and host cells is important for exploring the complex pathological mechanisms of classical swine fever (CSF). In the current stud...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00691/full |
| _version_ | 1819012075334139904 |
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| author | Jinding Chen Hongchao Gou Mingqiu Zhao Jin Yuan Hailuan Xu Hongxing Ding |
| author_facet | Jinding Chen Hongchao Gou Mingqiu Zhao Jin Yuan Hailuan Xu Hongxing Ding |
| author_sort | Jinding Chen |
| collection | DOAJ |
| description | Viruses require energy and biosynthetic precursors from host cells for replication. An understanding of the metabolic interplay between classical swine fever virus (CSFV) and host cells is important for exploring the complex pathological mechanisms of classical swine fever (CSF). In the current study, and for the first time, we utilized an approach involving gas chromatography coupled with mass spectrometry (GC-MS) to examine the metabolic profiles within PK-15 and 3D4/2 cells infected with CSFV. The differential metabolites of PK-15 cells caused by CSFV infection mainly included the decreased levels of glucose 6-phosphate [fold change (FC) = −1.94)] and glyceraldehyde-3-phosphate (FC = −1.83) during glycolysis, ribulose 5-phosphate (FC = −1.51) in the pentose phosphate pathway, guanosine (FC = −1.24) and inosine (FC = −1.16) during purine biosynthesis, but the increased levels of 2-ketoisovaleric acid (FC = 0.63) during the citrate cycle, and ornithine (FC = 0.56) and proline (FC = 0.62) during arginine and proline metabolism. However, metabolite changes caused by CSFV infection in 3D4/2 cells included the reduced glyceraldehyde-3-phosphate (FC = −0.77) and pyruvic acid (FC = −1.42) during glycolysis, 2-ketoglutaric acid (FC = −1.52) in the citrate cycle, and the elevated cytosine (FC = 2.15) during pyrimidine metabolism. Our data showed that CSFV might rebuild cellular metabolic programs, thus aiding viral replication. These findings may be important in developing targets for new biomarkers for the diagnosis and identification of enzyme inhibitors or metabolites as antiviral drugs, or screening viral gene products as vaccines. |
| first_indexed | 2024-12-21T01:38:17Z |
| format | Article |
| id | doaj.art-628a8996d223478fb5a24787635ff209 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T01:38:17Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-628a8996d223478fb5a24787635ff2092022-12-21T19:20:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-04-01810.3389/fmicb.2017.00691253056Metabolic Profiles in Cell Lines Infected with Classical Swine Fever VirusJinding ChenHongchao GouMingqiu ZhaoJin YuanHailuan XuHongxing DingViruses require energy and biosynthetic precursors from host cells for replication. An understanding of the metabolic interplay between classical swine fever virus (CSFV) and host cells is important for exploring the complex pathological mechanisms of classical swine fever (CSF). In the current study, and for the first time, we utilized an approach involving gas chromatography coupled with mass spectrometry (GC-MS) to examine the metabolic profiles within PK-15 and 3D4/2 cells infected with CSFV. The differential metabolites of PK-15 cells caused by CSFV infection mainly included the decreased levels of glucose 6-phosphate [fold change (FC) = −1.94)] and glyceraldehyde-3-phosphate (FC = −1.83) during glycolysis, ribulose 5-phosphate (FC = −1.51) in the pentose phosphate pathway, guanosine (FC = −1.24) and inosine (FC = −1.16) during purine biosynthesis, but the increased levels of 2-ketoisovaleric acid (FC = 0.63) during the citrate cycle, and ornithine (FC = 0.56) and proline (FC = 0.62) during arginine and proline metabolism. However, metabolite changes caused by CSFV infection in 3D4/2 cells included the reduced glyceraldehyde-3-phosphate (FC = −0.77) and pyruvic acid (FC = −1.42) during glycolysis, 2-ketoglutaric acid (FC = −1.52) in the citrate cycle, and the elevated cytosine (FC = 2.15) during pyrimidine metabolism. Our data showed that CSFV might rebuild cellular metabolic programs, thus aiding viral replication. These findings may be important in developing targets for new biomarkers for the diagnosis and identification of enzyme inhibitors or metabolites as antiviral drugs, or screening viral gene products as vaccines.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00691/fullmetabolomicsCSFVPK-15 cells3D4/2classical swine fever virus |
| spellingShingle | Jinding Chen Hongchao Gou Mingqiu Zhao Jin Yuan Hailuan Xu Hongxing Ding Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus Frontiers in Microbiology metabolomics CSFV PK-15 cells 3D4/2 classical swine fever virus |
| title | Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus |
| title_full | Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus |
| title_fullStr | Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus |
| title_full_unstemmed | Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus |
| title_short | Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus |
| title_sort | metabolic profiles in cell lines infected with classical swine fever virus |
| topic | metabolomics CSFV PK-15 cells 3D4/2 classical swine fever virus |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00691/full |
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