Isoflavones and probiotics effect on bone calcium and bone cells in rats
Isoflavones and probiotics have shown the therapeutic potential to alter calcium absorption and bone cell metabolism. This study sought to ascertain the effect of isoflavones and probiotics on calcium status and bone health in healthy female rats. Forty-eight adult female Wistar rats were grouped an...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023040082 |
| _version_ | 1797814342354206720 |
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| author | Iskandar Azmy Harahap Maciej Kuligowski Marcin Schmidt Paweł Kurzawa Ewa Pruszyńska-Oszmałek Maciej Sassek Joanna Suliburska |
| author_facet | Iskandar Azmy Harahap Maciej Kuligowski Marcin Schmidt Paweł Kurzawa Ewa Pruszyńska-Oszmałek Maciej Sassek Joanna Suliburska |
| author_sort | Iskandar Azmy Harahap |
| collection | DOAJ |
| description | Isoflavones and probiotics have shown the therapeutic potential to alter calcium absorption and bone cell metabolism. This study sought to ascertain the effect of isoflavones and probiotics on calcium status and bone health in healthy female rats. Forty-eight adult female Wistar rats were grouped and fed: a standard diet (control); and standard diets with tempeh; soy; daidzein and genistein; Lactobacillus acidophilus; and a combination of daidzein, genistein, and L. acidophilus. The biochemical serum parameters, such as alanine transaminase, aspartate transaminase, glucose, and triacylglycerol concentrations, were measured, and calcium contents in tissues were determined. After staining the bone with hematoxylin and eosin, the number of osteoblasts, osteocytes, and the percentage of bone marrow adipocytes were counted. Compared with the control group, the soy group showed a significantly lower triacylglycerol concentration. The L. acidophilus group considerably increased the calcium content in the femoral bone. The daidzein and genistein, L. acidophilus, and a combination of daidzein, genistein, and L. acidophilus groups showed significantly lower calcium contents in the heart and kidneys. The daidzein and genistein group significantly enhanced the number of osteoblasts and osteocytes. A substantial inverse correlation was observed between calcium contents in kidneys and osteoblasts. In conclusion, the combination of daidzein, genistein, and L. acidophilus may improve bone calcium concentrations and bone cells. However, no synergistic effect between isoflavones and probiotics was detected in this study. |
| first_indexed | 2024-03-13T08:06:16Z |
| format | Article |
| id | doaj.art-628fe30b66384ca3bcfa2014c1f26d7c |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:06:16Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-628fe30b66384ca3bcfa2014c1f26d7c2023-06-01T04:36:40ZengElsevierHeliyon2405-84402023-06-0196e16801Isoflavones and probiotics effect on bone calcium and bone cells in ratsIskandar Azmy Harahap0Maciej Kuligowski1Marcin Schmidt2Paweł Kurzawa3Ewa Pruszyńska-Oszmałek4Maciej Sassek5Joanna Suliburska6Department of Human Nutrition and Dietetics, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poznań, PolandDepartment of Food Technology of Plant Origin, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poznań, PolandDepartment of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poznań, PolandDepartment of Clinical Pathology, Poznań University of Medical Sciences, Poznań, PolandDepartment of Animal Physiology, Biochemistry and Biostructure, Faculty of Veterinary Medicine and Animal Science, Poznań University of Life Sciences, Poznań, PolandDepartment of Animal Physiology, Biochemistry and Biostructure, Faculty of Veterinary Medicine and Animal Science, Poznań University of Life Sciences, Poznań, PolandDepartment of Human Nutrition and Dietetics, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poznań, Poland; Corresponding author.Isoflavones and probiotics have shown the therapeutic potential to alter calcium absorption and bone cell metabolism. This study sought to ascertain the effect of isoflavones and probiotics on calcium status and bone health in healthy female rats. Forty-eight adult female Wistar rats were grouped and fed: a standard diet (control); and standard diets with tempeh; soy; daidzein and genistein; Lactobacillus acidophilus; and a combination of daidzein, genistein, and L. acidophilus. The biochemical serum parameters, such as alanine transaminase, aspartate transaminase, glucose, and triacylglycerol concentrations, were measured, and calcium contents in tissues were determined. After staining the bone with hematoxylin and eosin, the number of osteoblasts, osteocytes, and the percentage of bone marrow adipocytes were counted. Compared with the control group, the soy group showed a significantly lower triacylglycerol concentration. The L. acidophilus group considerably increased the calcium content in the femoral bone. The daidzein and genistein, L. acidophilus, and a combination of daidzein, genistein, and L. acidophilus groups showed significantly lower calcium contents in the heart and kidneys. The daidzein and genistein group significantly enhanced the number of osteoblasts and osteocytes. A substantial inverse correlation was observed between calcium contents in kidneys and osteoblasts. In conclusion, the combination of daidzein, genistein, and L. acidophilus may improve bone calcium concentrations and bone cells. However, no synergistic effect between isoflavones and probiotics was detected in this study.http://www.sciencedirect.com/science/article/pii/S2405844023040082IsoflavonesLactobacillus acidophilusCalcium statusBone cellsRats |
| spellingShingle | Iskandar Azmy Harahap Maciej Kuligowski Marcin Schmidt Paweł Kurzawa Ewa Pruszyńska-Oszmałek Maciej Sassek Joanna Suliburska Isoflavones and probiotics effect on bone calcium and bone cells in rats Heliyon Isoflavones Lactobacillus acidophilus Calcium status Bone cells Rats |
| title | Isoflavones and probiotics effect on bone calcium and bone cells in rats |
| title_full | Isoflavones and probiotics effect on bone calcium and bone cells in rats |
| title_fullStr | Isoflavones and probiotics effect on bone calcium and bone cells in rats |
| title_full_unstemmed | Isoflavones and probiotics effect on bone calcium and bone cells in rats |
| title_short | Isoflavones and probiotics effect on bone calcium and bone cells in rats |
| title_sort | isoflavones and probiotics effect on bone calcium and bone cells in rats |
| topic | Isoflavones Lactobacillus acidophilus Calcium status Bone cells Rats |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023040082 |
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