Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma
BackgroundHepatocellular carcinoma (HCC) is one of the most common types of cancer that is associated with poor quality of life in patients and a global health burden. The mechanisms involved in the development and progression of HCC remain poorly understood.MethodsHepatocellular carcinoma human sam...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.607636/full |
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| author | Tao Ma Xujun Zhou Hailiang Wei Shuguang Yan Yi Hui Yonggang Liu Hui Guo Qian Li Jingtao Li Zhanjie Chang Xiao-Xin Mu Xiao-Xin Mu |
| author_facet | Tao Ma Xujun Zhou Hailiang Wei Shuguang Yan Yi Hui Yonggang Liu Hui Guo Qian Li Jingtao Li Zhanjie Chang Xiao-Xin Mu Xiao-Xin Mu |
| author_sort | Tao Ma |
| collection | DOAJ |
| description | BackgroundHepatocellular carcinoma (HCC) is one of the most common types of cancer that is associated with poor quality of life in patients and a global health burden. The mechanisms involved in the development and progression of HCC remain poorly understood.MethodsHepatocellular carcinoma human samples and cell lines were subjected to qRT-PCR for expression assessment. CCK-8 assay, Transwell migration and invasion assay, were applied for cell function detection. Animal experiment was used to measure the function of SNHG17 on cell growth in vivo. Western blot was conducted to evaluate the level of EMT in cells. RIP, RNA pull-down and luciferase reporter assays were performed to assess the correlation between SNHG17, miR-3180-3p and RFX1.ResultsOur study demonstrated that SNHG17 was upregulated in HCC human samples and involved cell proliferation, migration, invasion progress. SNHG17 promoted HCC cell growth and metastasis in vivo. Furthermore, we investigated the downstream factor of SNHG17, SNHG17 acted as a molecular sponge for miR-3180-3p, and SNHG17 regulated RFX1 expression via miR-3180-3p. SNHG17 promotes tumor-like behavior in HCC cells via miR-3180-3p/RFX1.ConclusionWe determined RFX1 as the target of miR-3810-3p; SNHG17 enhanced the progression of HCC via the miR-3180-3p/RFX1 axis. Taken together, our findings may provide insight into the molecular mechanism involved in the progression of HCC and develop SNHG17 as a novel therapeutic target against HCC. |
| first_indexed | 2024-12-20T11:54:07Z |
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| language | English |
| last_indexed | 2024-12-20T11:54:07Z |
| publishDate | 2021-01-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-629012d6d6f84aea8658191e9ccc5ed92022-12-21T19:41:43ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-01-011110.3389/fgene.2020.607636607636Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular CarcinomaTao Ma0Xujun Zhou1Hailiang Wei2Shuguang Yan3Yi Hui4Yonggang Liu5Hui Guo6Qian Li7Jingtao Li8Zhanjie Chang9Xiao-Xin Mu10Xiao-Xin Mu11Department of Clinical Laboratory, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Gastroenterology, Wuhan Eighth Hospital, Wuhan, ChinaDepartment of General Surgery, The Hospital Affiliated to Shaanxi University of Chinese Medicine, Xianyang, ChinaCollege of Basic Medicine, The Shaanxi University of Chinese Medicine, Xianyang, ChinaCollege of Basic Medicine, The Shaanxi University of Chinese Medicine, Xianyang, ChinaDepartment of Liver Diseases, The Hospital Affiliated to Shaanxi University of Chinese Medicine, Xianyang, ChinaDepartment of General Surgery, The Hospital Affiliated to Shaanxi University of Chinese Medicine, Xianyang, ChinaMedical Experiment Center, The Shaanxi University of Chinese Medicine, Xianyang, ChinaDepartment of Liver Diseases, The Hospital Affiliated to Shaanxi University of Chinese Medicine, Xianyang, ChinaDepartment of Liver Diseases, The Hospital Affiliated to Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, Nanjing, ChinaNational Health Council (NHC) Key Laboratory of Living Donor Liver Transplantation, Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaBackgroundHepatocellular carcinoma (HCC) is one of the most common types of cancer that is associated with poor quality of life in patients and a global health burden. The mechanisms involved in the development and progression of HCC remain poorly understood.MethodsHepatocellular carcinoma human samples and cell lines were subjected to qRT-PCR for expression assessment. CCK-8 assay, Transwell migration and invasion assay, were applied for cell function detection. Animal experiment was used to measure the function of SNHG17 on cell growth in vivo. Western blot was conducted to evaluate the level of EMT in cells. RIP, RNA pull-down and luciferase reporter assays were performed to assess the correlation between SNHG17, miR-3180-3p and RFX1.ResultsOur study demonstrated that SNHG17 was upregulated in HCC human samples and involved cell proliferation, migration, invasion progress. SNHG17 promoted HCC cell growth and metastasis in vivo. Furthermore, we investigated the downstream factor of SNHG17, SNHG17 acted as a molecular sponge for miR-3180-3p, and SNHG17 regulated RFX1 expression via miR-3180-3p. SNHG17 promotes tumor-like behavior in HCC cells via miR-3180-3p/RFX1.ConclusionWe determined RFX1 as the target of miR-3810-3p; SNHG17 enhanced the progression of HCC via the miR-3180-3p/RFX1 axis. Taken together, our findings may provide insight into the molecular mechanism involved in the progression of HCC and develop SNHG17 as a novel therapeutic target against HCC.https://www.frontiersin.org/articles/10.3389/fgene.2020.607636/fulllncRNASNHG17miR-3180-3pRFX1hepatocellular carcinoma |
| spellingShingle | Tao Ma Xujun Zhou Hailiang Wei Shuguang Yan Yi Hui Yonggang Liu Hui Guo Qian Li Jingtao Li Zhanjie Chang Xiao-Xin Mu Xiao-Xin Mu Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma Frontiers in Genetics lncRNA SNHG17 miR-3180-3p RFX1 hepatocellular carcinoma |
| title | Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma |
| title_full | Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma |
| title_fullStr | Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma |
| title_full_unstemmed | Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma |
| title_short | Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma |
| title_sort | long non coding rna snhg17 upregulates rfx1 by sponging mir 3180 3p and promotes cellular function in hepatocellular carcinoma |
| topic | lncRNA SNHG17 miR-3180-3p RFX1 hepatocellular carcinoma |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2020.607636/full |
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