Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage
Using data independent acquisition (DIA) proteomics, the composition and biological functions of differentially expressed proteins in raw milk during cold storage at 4 ℃ for up to 6 days were investigated. A total of 902 proteins were detected. Among them, 70 and 71 differentially expressed proteins...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2024-03-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-5-027.pdf |
| _version_ | 1797202812800598016 |
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| author | ZHUANG Jiao, JU Ning, ZHU Xiaoxue, LI Yafeng, DING Yuhong, GAO Yan, CHEN Yanhui, QI Jin, YANG Bingkun |
| author_facet | ZHUANG Jiao, JU Ning, ZHU Xiaoxue, LI Yafeng, DING Yuhong, GAO Yan, CHEN Yanhui, QI Jin, YANG Bingkun |
| author_sort | ZHUANG Jiao, JU Ning, ZHU Xiaoxue, LI Yafeng, DING Yuhong, GAO Yan, CHEN Yanhui, QI Jin, YANG Bingkun |
| collection | DOAJ |
| description | Using data independent acquisition (DIA) proteomics, the composition and biological functions of differentially expressed proteins in raw milk during cold storage at 4 ℃ for up to 6 days were investigated. A total of 902 proteins were detected. Among them, 70 and 71 differentially expressed proteins were selected during the early (day 0 vs day 3) and late (day 3 vs day 6) stages of refrigeration, respectively. The differentially expressed proteins played an important role in the response to nerve growth factors and the regulation of cells and tissues at the early stage of refrigeration, and were mainly involved in the biological processes of cells. At the late stage of refrigeration, the differentially expressed proteins could regulate the metabolic processes of carbohydrate and RNA, and mainly participated in the sugar metabolic pathways. Protein-protein interaction (PPI) network analysis indicated that cell division control protein 42 homolog (CDC42) was the hub node protein for both refrigeration periods, which was closely related to phagocytosis. These findings revealed the compositional changes and functional diversity of functions in raw milk proteins during refrigeration, which will provide a theoretical basis for the cold storage of raw milk and be of great significance for the quality control of raw milk. |
| first_indexed | 2024-04-24T08:09:23Z |
| format | Article |
| id | doaj.art-62913531702e4145a5db2220b923c26e |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-24T08:09:23Z |
| publishDate | 2024-03-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-62913531702e4145a5db2220b923c26e2024-04-17T07:13:21ZengChina Food Publishing CompanyShipin Kexue1002-66302024-03-0145522523210.7506/spkx1002-6630-20230608-068Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold StorageZHUANG Jiao, JU Ning, ZHU Xiaoxue, LI Yafeng, DING Yuhong, GAO Yan, CHEN Yanhui, QI Jin, YANG Bingkun0(School of Food Science and Engineering, Ningxia University, Yinchuan 750021, China)Using data independent acquisition (DIA) proteomics, the composition and biological functions of differentially expressed proteins in raw milk during cold storage at 4 ℃ for up to 6 days were investigated. A total of 902 proteins were detected. Among them, 70 and 71 differentially expressed proteins were selected during the early (day 0 vs day 3) and late (day 3 vs day 6) stages of refrigeration, respectively. The differentially expressed proteins played an important role in the response to nerve growth factors and the regulation of cells and tissues at the early stage of refrigeration, and were mainly involved in the biological processes of cells. At the late stage of refrigeration, the differentially expressed proteins could regulate the metabolic processes of carbohydrate and RNA, and mainly participated in the sugar metabolic pathways. Protein-protein interaction (PPI) network analysis indicated that cell division control protein 42 homolog (CDC42) was the hub node protein for both refrigeration periods, which was closely related to phagocytosis. These findings revealed the compositional changes and functional diversity of functions in raw milk proteins during refrigeration, which will provide a theoretical basis for the cold storage of raw milk and be of great significance for the quality control of raw milk.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-5-027.pdfraw milk; refrigeration; proteomics; differentially expressed proteins; bioinformatics |
| spellingShingle | ZHUANG Jiao, JU Ning, ZHU Xiaoxue, LI Yafeng, DING Yuhong, GAO Yan, CHEN Yanhui, QI Jin, YANG Bingkun Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage Shipin Kexue raw milk; refrigeration; proteomics; differentially expressed proteins; bioinformatics |
| title | Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage |
| title_full | Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage |
| title_fullStr | Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage |
| title_full_unstemmed | Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage |
| title_short | Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage |
| title_sort | characterization and analysis of differentially expressed proteins in raw milk during cold storage |
| topic | raw milk; refrigeration; proteomics; differentially expressed proteins; bioinformatics |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-5-027.pdf |
| work_keys_str_mv | AT zhuangjiaojuningzhuxiaoxueliyafengdingyuhonggaoyanchenyanhuiqijinyangbingkun characterizationandanalysisofdifferentiallyexpressedproteinsinrawmilkduringcoldstorage |